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Comparative Transcriptome Analysis of the Cosmopolitan Marine Fungus Corollospora maritima Under Two Physiological Conditions.

Velez P, Alejandri-Ramírez ND, González MC, Estrada KJ, Sanchez-Flores A, Dinkova TD - G3 (Bethesda) (2015)

Bottom Line: The de novo reconstruction of C. maritima transcriptome Illumina sequencing provided a total of 14,530 transcripts (16 megabases).Using fungal isolates collected from different beaches, the specific environmental regulation of particular transcript differential expression was confirmed by RT-qPCR.To our knowledge, this is the first analysis that explores the marine fungus C. maritima molecular responses to overcome freshwater stress, and these data could shed light to understand the fungal adaptation and plasticity mechanisms to the marine habitat.

View Article: PubMed Central - PubMed

Affiliation: Departamento de Botánica, Instituto de Biología, Universidad Nacional Autónoma de México, Distrito Federal, México 04510 pvelezaguilar@gmail.com cesy@unam.mx.

No MeSH data available.


Related in: MedlinePlus

Expression analysis of selected genes during the exponential growth of C. maritima in freshwater and marine environments. The expression patterns of selected genes from C. maritima are shown as fold-change in marine compared to freshwater conditions. The expression was analyzed by RT-qPCR in three biological replicates representing fungal isolates from three different geographical locations, Pico de Oro Beach (A), Paraíso Beach (B), and Boca del Río Beach (C) at the growth point highlighted by an arrow in Figure 1. 133 (comp133_c0_seq1) is an isopenicillin epimerase component-like protein; 2003 (comp2003_c0_seq1), a multipass membrane protein; 1743 (comp1743_c0_seq1), an aspartic type endopeptidase; 1764 (comp1764_c0_seq1), a choline sulfatase family protein from the endoplasmic reticulum; 1325 (comp1325_c0_seq1), a glycosyl phosphatidyl inositol-anchored membrane protein with probable glucosidase activity; 343 (comp343_c0_seq1), a pathogenesis-related protein with CFEM domain; 1470 (comp1470_c0_seq1), a stress-related protein with homology to the ferritin-like superfamily. Black bars represent increased expression and gray bars decreased expression in a marine environment vs. a freshwater environment, according to the RNA-seq analysis. Error bars are shown for three technical replicates performed on each biological sample.
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fig4: Expression analysis of selected genes during the exponential growth of C. maritima in freshwater and marine environments. The expression patterns of selected genes from C. maritima are shown as fold-change in marine compared to freshwater conditions. The expression was analyzed by RT-qPCR in three biological replicates representing fungal isolates from three different geographical locations, Pico de Oro Beach (A), Paraíso Beach (B), and Boca del Río Beach (C) at the growth point highlighted by an arrow in Figure 1. 133 (comp133_c0_seq1) is an isopenicillin epimerase component-like protein; 2003 (comp2003_c0_seq1), a multipass membrane protein; 1743 (comp1743_c0_seq1), an aspartic type endopeptidase; 1764 (comp1764_c0_seq1), a choline sulfatase family protein from the endoplasmic reticulum; 1325 (comp1325_c0_seq1), a glycosyl phosphatidyl inositol-anchored membrane protein with probable glucosidase activity; 343 (comp343_c0_seq1), a pathogenesis-related protein with CFEM domain; 1470 (comp1470_c0_seq1), a stress-related protein with homology to the ferritin-like superfamily. Black bars represent increased expression and gray bars decreased expression in a marine environment vs. a freshwater environment, according to the RNA-seq analysis. Error bars are shown for three technical replicates performed on each biological sample.

Mentions: As observed in Figure 4, for the marine-induced transcripts (black bars) there was great consistency between replicates and with the RNA-seq results, although the relative expression ratios were generally greater in the RT-qPCR analysis. The IPN-like protein transcript or comp133_c0_seq1 consistently showed the greater induction in marine growth, regardless of the fungal isolate. Comp1743_c0_seq1 (ASP-PEP) and comp1764_c0_seq1 (CHS-ER) showed variations in the induction levels, depending on the fungal isolate. The fungal isolate corresponding to Figure 4A was the same as that used for RNA-seq (Pico de Oro Beach, Tabasco), whereas results in Figure 4B and Figure 4C corresponded to isolates from Paraíso Beach, Tabasco, and Boca del Río Beach, Veracruz, respectively.


Comparative Transcriptome Analysis of the Cosmopolitan Marine Fungus Corollospora maritima Under Two Physiological Conditions.

Velez P, Alejandri-Ramírez ND, González MC, Estrada KJ, Sanchez-Flores A, Dinkova TD - G3 (Bethesda) (2015)

Expression analysis of selected genes during the exponential growth of C. maritima in freshwater and marine environments. The expression patterns of selected genes from C. maritima are shown as fold-change in marine compared to freshwater conditions. The expression was analyzed by RT-qPCR in three biological replicates representing fungal isolates from three different geographical locations, Pico de Oro Beach (A), Paraíso Beach (B), and Boca del Río Beach (C) at the growth point highlighted by an arrow in Figure 1. 133 (comp133_c0_seq1) is an isopenicillin epimerase component-like protein; 2003 (comp2003_c0_seq1), a multipass membrane protein; 1743 (comp1743_c0_seq1), an aspartic type endopeptidase; 1764 (comp1764_c0_seq1), a choline sulfatase family protein from the endoplasmic reticulum; 1325 (comp1325_c0_seq1), a glycosyl phosphatidyl inositol-anchored membrane protein with probable glucosidase activity; 343 (comp343_c0_seq1), a pathogenesis-related protein with CFEM domain; 1470 (comp1470_c0_seq1), a stress-related protein with homology to the ferritin-like superfamily. Black bars represent increased expression and gray bars decreased expression in a marine environment vs. a freshwater environment, according to the RNA-seq analysis. Error bars are shown for three technical replicates performed on each biological sample.
© Copyright Policy - open-access
Related In: Results  -  Collection

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fig4: Expression analysis of selected genes during the exponential growth of C. maritima in freshwater and marine environments. The expression patterns of selected genes from C. maritima are shown as fold-change in marine compared to freshwater conditions. The expression was analyzed by RT-qPCR in three biological replicates representing fungal isolates from three different geographical locations, Pico de Oro Beach (A), Paraíso Beach (B), and Boca del Río Beach (C) at the growth point highlighted by an arrow in Figure 1. 133 (comp133_c0_seq1) is an isopenicillin epimerase component-like protein; 2003 (comp2003_c0_seq1), a multipass membrane protein; 1743 (comp1743_c0_seq1), an aspartic type endopeptidase; 1764 (comp1764_c0_seq1), a choline sulfatase family protein from the endoplasmic reticulum; 1325 (comp1325_c0_seq1), a glycosyl phosphatidyl inositol-anchored membrane protein with probable glucosidase activity; 343 (comp343_c0_seq1), a pathogenesis-related protein with CFEM domain; 1470 (comp1470_c0_seq1), a stress-related protein with homology to the ferritin-like superfamily. Black bars represent increased expression and gray bars decreased expression in a marine environment vs. a freshwater environment, according to the RNA-seq analysis. Error bars are shown for three technical replicates performed on each biological sample.
Mentions: As observed in Figure 4, for the marine-induced transcripts (black bars) there was great consistency between replicates and with the RNA-seq results, although the relative expression ratios were generally greater in the RT-qPCR analysis. The IPN-like protein transcript or comp133_c0_seq1 consistently showed the greater induction in marine growth, regardless of the fungal isolate. Comp1743_c0_seq1 (ASP-PEP) and comp1764_c0_seq1 (CHS-ER) showed variations in the induction levels, depending on the fungal isolate. The fungal isolate corresponding to Figure 4A was the same as that used for RNA-seq (Pico de Oro Beach, Tabasco), whereas results in Figure 4B and Figure 4C corresponded to isolates from Paraíso Beach, Tabasco, and Boca del Río Beach, Veracruz, respectively.

Bottom Line: The de novo reconstruction of C. maritima transcriptome Illumina sequencing provided a total of 14,530 transcripts (16 megabases).Using fungal isolates collected from different beaches, the specific environmental regulation of particular transcript differential expression was confirmed by RT-qPCR.To our knowledge, this is the first analysis that explores the marine fungus C. maritima molecular responses to overcome freshwater stress, and these data could shed light to understand the fungal adaptation and plasticity mechanisms to the marine habitat.

View Article: PubMed Central - PubMed

Affiliation: Departamento de Botánica, Instituto de Biología, Universidad Nacional Autónoma de México, Distrito Federal, México 04510 pvelezaguilar@gmail.com cesy@unam.mx.

No MeSH data available.


Related in: MedlinePlus