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Comparative Transcriptome Analysis of the Cosmopolitan Marine Fungus Corollospora maritima Under Two Physiological Conditions.

Velez P, Alejandri-Ramírez ND, González MC, Estrada KJ, Sanchez-Flores A, Dinkova TD - G3 (Bethesda) (2015)

Bottom Line: The de novo reconstruction of C. maritima transcriptome Illumina sequencing provided a total of 14,530 transcripts (16 megabases).Using fungal isolates collected from different beaches, the specific environmental regulation of particular transcript differential expression was confirmed by RT-qPCR.To our knowledge, this is the first analysis that explores the marine fungus C. maritima molecular responses to overcome freshwater stress, and these data could shed light to understand the fungal adaptation and plasticity mechanisms to the marine habitat.

View Article: PubMed Central - PubMed

Affiliation: Departamento de Botánica, Instituto de Biología, Universidad Nacional Autónoma de México, Distrito Federal, México 04510 pvelezaguilar@gmail.com cesy@unam.mx.

No MeSH data available.


Related in: MedlinePlus

Graphical representation of C. maritima growth under two salinity conditions (bars represent SD). The growth in freshwater is represented by filled squares, whereas the marine growth is represented as the solid circles. Statistical significance between the curves was assessed using a permutation test to compare growth curves. The test was applied to the colony diameter until effects on the growth were most apparent, that is, 16–19 d after inoculation. The pair-wise comparisons between the fungi samples were statistically nonsignificant (P-value 0.0999). The arrows indicate the time of growth from where the RNA isolation was performed for either the transcriptome or the RT-qPCR analysis.
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fig1: Graphical representation of C. maritima growth under two salinity conditions (bars represent SD). The growth in freshwater is represented by filled squares, whereas the marine growth is represented as the solid circles. Statistical significance between the curves was assessed using a permutation test to compare growth curves. The test was applied to the colony diameter until effects on the growth were most apparent, that is, 16–19 d after inoculation. The pair-wise comparisons between the fungi samples were statistically nonsignificant (P-value 0.0999). The arrows indicate the time of growth from where the RNA isolation was performed for either the transcriptome or the RT-qPCR analysis.

Mentions: Total RNA was obtained from samples at the mid-log phase of vegetative growth (15 d) for both freshwater-grown and seawater-grown tissues (Figure 1). Frozen aliquots of mycelia were quickly ground in Trizol reagent (Invitrogen Corporation, California, USA) and RNA was subsequently isolated following the manufacturer’s instructions. The integrity of RNA was determined using the Agilent 2100 Bioanalyzer system prior to the library preparation. All the samples presented a minimum RNA Integrity Number (RIN) of eight.


Comparative Transcriptome Analysis of the Cosmopolitan Marine Fungus Corollospora maritima Under Two Physiological Conditions.

Velez P, Alejandri-Ramírez ND, González MC, Estrada KJ, Sanchez-Flores A, Dinkova TD - G3 (Bethesda) (2015)

Graphical representation of C. maritima growth under two salinity conditions (bars represent SD). The growth in freshwater is represented by filled squares, whereas the marine growth is represented as the solid circles. Statistical significance between the curves was assessed using a permutation test to compare growth curves. The test was applied to the colony diameter until effects on the growth were most apparent, that is, 16–19 d after inoculation. The pair-wise comparisons between the fungi samples were statistically nonsignificant (P-value 0.0999). The arrows indicate the time of growth from where the RNA isolation was performed for either the transcriptome or the RT-qPCR analysis.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4555217&req=5

fig1: Graphical representation of C. maritima growth under two salinity conditions (bars represent SD). The growth in freshwater is represented by filled squares, whereas the marine growth is represented as the solid circles. Statistical significance between the curves was assessed using a permutation test to compare growth curves. The test was applied to the colony diameter until effects on the growth were most apparent, that is, 16–19 d after inoculation. The pair-wise comparisons between the fungi samples were statistically nonsignificant (P-value 0.0999). The arrows indicate the time of growth from where the RNA isolation was performed for either the transcriptome or the RT-qPCR analysis.
Mentions: Total RNA was obtained from samples at the mid-log phase of vegetative growth (15 d) for both freshwater-grown and seawater-grown tissues (Figure 1). Frozen aliquots of mycelia were quickly ground in Trizol reagent (Invitrogen Corporation, California, USA) and RNA was subsequently isolated following the manufacturer’s instructions. The integrity of RNA was determined using the Agilent 2100 Bioanalyzer system prior to the library preparation. All the samples presented a minimum RNA Integrity Number (RIN) of eight.

Bottom Line: The de novo reconstruction of C. maritima transcriptome Illumina sequencing provided a total of 14,530 transcripts (16 megabases).Using fungal isolates collected from different beaches, the specific environmental regulation of particular transcript differential expression was confirmed by RT-qPCR.To our knowledge, this is the first analysis that explores the marine fungus C. maritima molecular responses to overcome freshwater stress, and these data could shed light to understand the fungal adaptation and plasticity mechanisms to the marine habitat.

View Article: PubMed Central - PubMed

Affiliation: Departamento de Botánica, Instituto de Biología, Universidad Nacional Autónoma de México, Distrito Federal, México 04510 pvelezaguilar@gmail.com cesy@unam.mx.

No MeSH data available.


Related in: MedlinePlus