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High-Dose Menaquinone-7 Supplementation Reduces Cardiovascular Calcification in a Murine Model of Extraosseous Calcification.

Scheiber D, Veulemans V, Horn P, Chatrou ML, Potthoff SA, Kelm M, Schurgers LJ, Westenfeld R - Nutrients (2015)

Bottom Line: Furthermore, MK-7 supplementation increased aortic MGP messenger ribonucleic acid (mRNA) expression (10-fold; p < 0.05).CKD-induced arterial hypertension with secondary myocardial hypertrophy and increased elastic fiber breaking points in the arterial tunica media did not change with MK-7 supplementation.The protective effect of MK-7 may be related to the inhibition of secondary mineralization of damaged vascular structures.

View Article: PubMed Central - PubMed

Affiliation: Division of Cardiology, Pulmonology, and Vascular Medicine, Medical Faculty, University Duesseldorf, Duesseldorf 40225, Germany. daniel.scheiber@med.uni-duesseldorf.de.

ABSTRACT
Cardiovascular calcification is prevalent in the aging population and in patients with chronic kidney disease (CKD) and diabetes mellitus, giving rise to substantial morbidity and mortality. Vitamin K-dependent matrix Gla-protein (MGP) is an important inhibitor of calcification. The aim of this study was to evaluate the impact of high-dose menaquinone-7 (MK-7) supplementation (100 µg/g diet) on the development of extraosseous calcification in a murine model. Calcification was induced by 5/6 nephrectomy combined with high phosphate diet in rats. Sham operated animals served as controls. Animals received high or low MK-7 diets for 12 weeks. We assessed vital parameters, serum chemistry, creatinine clearance, and cardiac function. CKD provoked increased aortic (1.3 fold; p < 0.05) and myocardial (2.4 fold; p < 0.05) calcification in line with increased alkaline phosphatase levels (2.2 fold; p < 0.01). MK-7 supplementation inhibited cardiovascular calcification and decreased aortic alkaline phosphatase tissue concentrations. Furthermore, MK-7 supplementation increased aortic MGP messenger ribonucleic acid (mRNA) expression (10-fold; p < 0.05). CKD-induced arterial hypertension with secondary myocardial hypertrophy and increased elastic fiber breaking points in the arterial tunica media did not change with MK-7 supplementation. Our results show that high-dose MK-7 supplementation inhibits the development of cardiovascular calcification. The protective effect of MK-7 may be related to the inhibition of secondary mineralization of damaged vascular structures.

No MeSH data available.


Related in: MedlinePlus

Systolic and diastolic blood pressure. Systolic and diastolic blood pressure after 12 weeks of treatment. CKD animals developed significantly increased systolic and diastolic blood pressure values compared to controls. Co = control group; Co-K2 = MK-7 supplemented control group; CKD = 5/6 nephrectomized group; CKD-K2 = MK-7 supplemented 5/6 nephrectomized group; mmHg = millimeters of mercury. Significant differences: *p < 0.05, **p < 0.01.
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nutrients-07-05318-f009: Systolic and diastolic blood pressure. Systolic and diastolic blood pressure after 12 weeks of treatment. CKD animals developed significantly increased systolic and diastolic blood pressure values compared to controls. Co = control group; Co-K2 = MK-7 supplemented control group; CKD = 5/6 nephrectomized group; CKD-K2 = MK-7 supplemented 5/6 nephrectomized group; mmHg = millimeters of mercury. Significant differences: *p < 0.05, **p < 0.01.

Mentions: Blood pressure: CKD and CKD-K2 animals developed arterial hypertension (Figure 9). After 12 weeks, systolic as well as diastolic blood pressure values were elevated by 15–20 mmHg in CKD rats compared to controls (CKD-K2vs. Co-K2, p < 0.01). MK-7 supplementation did not affect blood pressure (Figure 9).


High-Dose Menaquinone-7 Supplementation Reduces Cardiovascular Calcification in a Murine Model of Extraosseous Calcification.

Scheiber D, Veulemans V, Horn P, Chatrou ML, Potthoff SA, Kelm M, Schurgers LJ, Westenfeld R - Nutrients (2015)

Systolic and diastolic blood pressure. Systolic and diastolic blood pressure after 12 weeks of treatment. CKD animals developed significantly increased systolic and diastolic blood pressure values compared to controls. Co = control group; Co-K2 = MK-7 supplemented control group; CKD = 5/6 nephrectomized group; CKD-K2 = MK-7 supplemented 5/6 nephrectomized group; mmHg = millimeters of mercury. Significant differences: *p < 0.05, **p < 0.01.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4555157&req=5

nutrients-07-05318-f009: Systolic and diastolic blood pressure. Systolic and diastolic blood pressure after 12 weeks of treatment. CKD animals developed significantly increased systolic and diastolic blood pressure values compared to controls. Co = control group; Co-K2 = MK-7 supplemented control group; CKD = 5/6 nephrectomized group; CKD-K2 = MK-7 supplemented 5/6 nephrectomized group; mmHg = millimeters of mercury. Significant differences: *p < 0.05, **p < 0.01.
Mentions: Blood pressure: CKD and CKD-K2 animals developed arterial hypertension (Figure 9). After 12 weeks, systolic as well as diastolic blood pressure values were elevated by 15–20 mmHg in CKD rats compared to controls (CKD-K2vs. Co-K2, p < 0.01). MK-7 supplementation did not affect blood pressure (Figure 9).

Bottom Line: Furthermore, MK-7 supplementation increased aortic MGP messenger ribonucleic acid (mRNA) expression (10-fold; p < 0.05).CKD-induced arterial hypertension with secondary myocardial hypertrophy and increased elastic fiber breaking points in the arterial tunica media did not change with MK-7 supplementation.The protective effect of MK-7 may be related to the inhibition of secondary mineralization of damaged vascular structures.

View Article: PubMed Central - PubMed

Affiliation: Division of Cardiology, Pulmonology, and Vascular Medicine, Medical Faculty, University Duesseldorf, Duesseldorf 40225, Germany. daniel.scheiber@med.uni-duesseldorf.de.

ABSTRACT
Cardiovascular calcification is prevalent in the aging population and in patients with chronic kidney disease (CKD) and diabetes mellitus, giving rise to substantial morbidity and mortality. Vitamin K-dependent matrix Gla-protein (MGP) is an important inhibitor of calcification. The aim of this study was to evaluate the impact of high-dose menaquinone-7 (MK-7) supplementation (100 µg/g diet) on the development of extraosseous calcification in a murine model. Calcification was induced by 5/6 nephrectomy combined with high phosphate diet in rats. Sham operated animals served as controls. Animals received high or low MK-7 diets for 12 weeks. We assessed vital parameters, serum chemistry, creatinine clearance, and cardiac function. CKD provoked increased aortic (1.3 fold; p < 0.05) and myocardial (2.4 fold; p < 0.05) calcification in line with increased alkaline phosphatase levels (2.2 fold; p < 0.01). MK-7 supplementation inhibited cardiovascular calcification and decreased aortic alkaline phosphatase tissue concentrations. Furthermore, MK-7 supplementation increased aortic MGP messenger ribonucleic acid (mRNA) expression (10-fold; p < 0.05). CKD-induced arterial hypertension with secondary myocardial hypertrophy and increased elastic fiber breaking points in the arterial tunica media did not change with MK-7 supplementation. Our results show that high-dose MK-7 supplementation inhibits the development of cardiovascular calcification. The protective effect of MK-7 may be related to the inhibition of secondary mineralization of damaged vascular structures.

No MeSH data available.


Related in: MedlinePlus