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Determination of Free Radical Scavenging, Antioxidative DNA Damage Activities and Phytochemical Components of Active Fractions from Lansium domesticum Corr. Fruit.

Klungsupya P, Suthepakul N, Muangman T, Rerk-Am U, Thongdon-A J - Nutrients (2015)

Bottom Line: The comet results revealed DNA-protective activity of both LDSK50-EA and LDSK50-H2O fractions when TK6 human lymphoblast cells were pre-treated at 25, 50, 100, and 200 μg/mL for 24 h prior to H2O2 exposure.This study generates new information on the biological activity of L. domesticum.It will promote and strengthen the utilization of L. domesticum by-products.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmaceuticals and Natural Products, Thailand Institute of Scientific and Technological Research (TISTR), 35 Mu 3 Techno Polis, Khlong Luang, Pathum Thani 12120, Thailand. prapaipat@tistr.or.th.

ABSTRACT
Lansium domesticum Corr. or "long-kong" is one of the most popular fruits in Thailand. Its peel (skin, SK) and seeds (SD) become waste unless recycled or applied for use. This study was undertaken to determine the bioactivity and phytochemical components of L. domesticum (LD) skin and seed extracts. Following various extraction and fractionation procedures, 12 fractions were obtained. All fractions were tested for antioxidant capacity against O2(-•) and OH(•). It was found that the peel of L. domesticum fruits exhibited higher O2(-•) and OH(•) scavenging activity than seeds. High potential antioxidant activity was found in two fractions of 50% ethanol extract of peel followed by ethyl acetate (EA) fractionation (LDSK50-EA) and its aqueous phase (LDSK50-H2O). Therefore, these two active fractions were selected for further studies on their antioxidative activity against DNA damage by hydrogen peroxide (H2O2) in human TK6 cells using comet assay. The comet results revealed DNA-protective activity of both LDSK50-EA and LDSK50-H2O fractions when TK6 human lymphoblast cells were pre-treated at 25, 50, 100, and 200 μg/mL for 24 h prior to H2O2 exposure. The phytochemical analysis illustrated the presence of phenolic substances, mainly scopoletin, rutin, and chlorogenic acid, in these two active fractions. This study generates new information on the biological activity of L. domesticum. It will promote and strengthen the utilization of L. domesticum by-products.

No MeSH data available.


Related in: MedlinePlus

Thin Layer Chromatography (TLC) analysis of LDSK50-EA (fractions of 50% ethanol extract of the L. domesticum skin followed by ethyl acetate (EA) fractionation) fraction detected with Natural Product/polyethylene glycol (NP/PEG) spray reagent (366 nm) and against phytochemical standards including scopoletin (SCO), rutin (RU), and chlorogenic acid (CHLO). (a) toluene:ethyl acetate:formic acid (5:4:1) system; (b) and (c) ethyl acetate:formic acid:acetic acid:water (137:11:11:26) system.
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nutrients-07-05312-f006: Thin Layer Chromatography (TLC) analysis of LDSK50-EA (fractions of 50% ethanol extract of the L. domesticum skin followed by ethyl acetate (EA) fractionation) fraction detected with Natural Product/polyethylene glycol (NP/PEG) spray reagent (366 nm) and against phytochemical standards including scopoletin (SCO), rutin (RU), and chlorogenic acid (CHLO). (a) toluene:ethyl acetate:formic acid (5:4:1) system; (b) and (c) ethyl acetate:formic acid:acetic acid:water (137:11:11:26) system.

Mentions: LDSK50-EA was dissolved in absolute ethanol at a concentration of 100 mg/mL, and spotted in 10–20 µL aliquots onto silica gel F254 plates. The developing solvents were System 1: toluene:ethyl acetate:formic acid (5:4:1) and System 2: ethyl acetate: formic acid: acetic acid: water (137:11:11:26). After development, the plates were dried and sprayed with PEG reagent. Bands were visualized under ultraviolet (UV) detector at 366 nm and their Rf values were recorded and compared with three standard phytochemicals including scopoletin, rutin, and chlorogenic acid. TLC analysis of LDSK50-EA was shown in Figure 6. Under the detecting condition used in this study, the results clearly revealed a presence of scopoletin (Rf 0.44), rutin (Rf 0.34), and chlorogenic acid (Rf 0.49) in LDSK50-EA.


Determination of Free Radical Scavenging, Antioxidative DNA Damage Activities and Phytochemical Components of Active Fractions from Lansium domesticum Corr. Fruit.

Klungsupya P, Suthepakul N, Muangman T, Rerk-Am U, Thongdon-A J - Nutrients (2015)

Thin Layer Chromatography (TLC) analysis of LDSK50-EA (fractions of 50% ethanol extract of the L. domesticum skin followed by ethyl acetate (EA) fractionation) fraction detected with Natural Product/polyethylene glycol (NP/PEG) spray reagent (366 nm) and against phytochemical standards including scopoletin (SCO), rutin (RU), and chlorogenic acid (CHLO). (a) toluene:ethyl acetate:formic acid (5:4:1) system; (b) and (c) ethyl acetate:formic acid:acetic acid:water (137:11:11:26) system.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4555151&req=5

nutrients-07-05312-f006: Thin Layer Chromatography (TLC) analysis of LDSK50-EA (fractions of 50% ethanol extract of the L. domesticum skin followed by ethyl acetate (EA) fractionation) fraction detected with Natural Product/polyethylene glycol (NP/PEG) spray reagent (366 nm) and against phytochemical standards including scopoletin (SCO), rutin (RU), and chlorogenic acid (CHLO). (a) toluene:ethyl acetate:formic acid (5:4:1) system; (b) and (c) ethyl acetate:formic acid:acetic acid:water (137:11:11:26) system.
Mentions: LDSK50-EA was dissolved in absolute ethanol at a concentration of 100 mg/mL, and spotted in 10–20 µL aliquots onto silica gel F254 plates. The developing solvents were System 1: toluene:ethyl acetate:formic acid (5:4:1) and System 2: ethyl acetate: formic acid: acetic acid: water (137:11:11:26). After development, the plates were dried and sprayed with PEG reagent. Bands were visualized under ultraviolet (UV) detector at 366 nm and their Rf values were recorded and compared with three standard phytochemicals including scopoletin, rutin, and chlorogenic acid. TLC analysis of LDSK50-EA was shown in Figure 6. Under the detecting condition used in this study, the results clearly revealed a presence of scopoletin (Rf 0.44), rutin (Rf 0.34), and chlorogenic acid (Rf 0.49) in LDSK50-EA.

Bottom Line: The comet results revealed DNA-protective activity of both LDSK50-EA and LDSK50-H2O fractions when TK6 human lymphoblast cells were pre-treated at 25, 50, 100, and 200 μg/mL for 24 h prior to H2O2 exposure.This study generates new information on the biological activity of L. domesticum.It will promote and strengthen the utilization of L. domesticum by-products.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmaceuticals and Natural Products, Thailand Institute of Scientific and Technological Research (TISTR), 35 Mu 3 Techno Polis, Khlong Luang, Pathum Thani 12120, Thailand. prapaipat@tistr.or.th.

ABSTRACT
Lansium domesticum Corr. or "long-kong" is one of the most popular fruits in Thailand. Its peel (skin, SK) and seeds (SD) become waste unless recycled or applied for use. This study was undertaken to determine the bioactivity and phytochemical components of L. domesticum (LD) skin and seed extracts. Following various extraction and fractionation procedures, 12 fractions were obtained. All fractions were tested for antioxidant capacity against O2(-•) and OH(•). It was found that the peel of L. domesticum fruits exhibited higher O2(-•) and OH(•) scavenging activity than seeds. High potential antioxidant activity was found in two fractions of 50% ethanol extract of peel followed by ethyl acetate (EA) fractionation (LDSK50-EA) and its aqueous phase (LDSK50-H2O). Therefore, these two active fractions were selected for further studies on their antioxidative activity against DNA damage by hydrogen peroxide (H2O2) in human TK6 cells using comet assay. The comet results revealed DNA-protective activity of both LDSK50-EA and LDSK50-H2O fractions when TK6 human lymphoblast cells were pre-treated at 25, 50, 100, and 200 μg/mL for 24 h prior to H2O2 exposure. The phytochemical analysis illustrated the presence of phenolic substances, mainly scopoletin, rutin, and chlorogenic acid, in these two active fractions. This study generates new information on the biological activity of L. domesticum. It will promote and strengthen the utilization of L. domesticum by-products.

No MeSH data available.


Related in: MedlinePlus