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Determination of Free Radical Scavenging, Antioxidative DNA Damage Activities and Phytochemical Components of Active Fractions from Lansium domesticum Corr. Fruit.

Klungsupya P, Suthepakul N, Muangman T, Rerk-Am U, Thongdon-A J - Nutrients (2015)

Bottom Line: The comet results revealed DNA-protective activity of both LDSK50-EA and LDSK50-H2O fractions when TK6 human lymphoblast cells were pre-treated at 25, 50, 100, and 200 μg/mL for 24 h prior to H2O2 exposure.This study generates new information on the biological activity of L. domesticum.It will promote and strengthen the utilization of L. domesticum by-products.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmaceuticals and Natural Products, Thailand Institute of Scientific and Technological Research (TISTR), 35 Mu 3 Techno Polis, Khlong Luang, Pathum Thani 12120, Thailand. prapaipat@tistr.or.th.

ABSTRACT
Lansium domesticum Corr. or "long-kong" is one of the most popular fruits in Thailand. Its peel (skin, SK) and seeds (SD) become waste unless recycled or applied for use. This study was undertaken to determine the bioactivity and phytochemical components of L. domesticum (LD) skin and seed extracts. Following various extraction and fractionation procedures, 12 fractions were obtained. All fractions were tested for antioxidant capacity against O2(-•) and OH(•). It was found that the peel of L. domesticum fruits exhibited higher O2(-•) and OH(•) scavenging activity than seeds. High potential antioxidant activity was found in two fractions of 50% ethanol extract of peel followed by ethyl acetate (EA) fractionation (LDSK50-EA) and its aqueous phase (LDSK50-H2O). Therefore, these two active fractions were selected for further studies on their antioxidative activity against DNA damage by hydrogen peroxide (H2O2) in human TK6 cells using comet assay. The comet results revealed DNA-protective activity of both LDSK50-EA and LDSK50-H2O fractions when TK6 human lymphoblast cells were pre-treated at 25, 50, 100, and 200 μg/mL for 24 h prior to H2O2 exposure. The phytochemical analysis illustrated the presence of phenolic substances, mainly scopoletin, rutin, and chlorogenic acid, in these two active fractions. This study generates new information on the biological activity of L. domesticum. It will promote and strengthen the utilization of L. domesticum by-products.

No MeSH data available.


Related in: MedlinePlus

The comet images of TK6 cells (from top to bottom) of the following treatments: control or untreated (undamaged DNA), 50 µM H2O2, 100 µg/mL Trolox (positive control), 200 µg/mL LDSK50-EA, and 200 µg/mL LDSK50-H2O. Cells were stained with ethidium bromide and taken by fluorescence microscope at medium (200×) and high (400×) magnification. LDSK50-EA: fractions of 50% ethanol extract of the L. domesticum skin followed by ethyl acetate (EA) fractionation; LDSK50-H2O: the aqueous phase product when the L. domesticum skin was extracted with 50% aqueous ethanol and partitioned with EA.
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nutrients-07-05312-f005: The comet images of TK6 cells (from top to bottom) of the following treatments: control or untreated (undamaged DNA), 50 µM H2O2, 100 µg/mL Trolox (positive control), 200 µg/mL LDSK50-EA, and 200 µg/mL LDSK50-H2O. Cells were stained with ethidium bromide and taken by fluorescence microscope at medium (200×) and high (400×) magnification. LDSK50-EA: fractions of 50% ethanol extract of the L. domesticum skin followed by ethyl acetate (EA) fractionation; LDSK50-H2O: the aqueous phase product when the L. domesticum skin was extracted with 50% aqueous ethanol and partitioned with EA.

Mentions: Fluorescence images of comet TK6 cells evaluated for the different treatment groups were demonstrated in Figure 5.


Determination of Free Radical Scavenging, Antioxidative DNA Damage Activities and Phytochemical Components of Active Fractions from Lansium domesticum Corr. Fruit.

Klungsupya P, Suthepakul N, Muangman T, Rerk-Am U, Thongdon-A J - Nutrients (2015)

The comet images of TK6 cells (from top to bottom) of the following treatments: control or untreated (undamaged DNA), 50 µM H2O2, 100 µg/mL Trolox (positive control), 200 µg/mL LDSK50-EA, and 200 µg/mL LDSK50-H2O. Cells were stained with ethidium bromide and taken by fluorescence microscope at medium (200×) and high (400×) magnification. LDSK50-EA: fractions of 50% ethanol extract of the L. domesticum skin followed by ethyl acetate (EA) fractionation; LDSK50-H2O: the aqueous phase product when the L. domesticum skin was extracted with 50% aqueous ethanol and partitioned with EA.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4555151&req=5

nutrients-07-05312-f005: The comet images of TK6 cells (from top to bottom) of the following treatments: control or untreated (undamaged DNA), 50 µM H2O2, 100 µg/mL Trolox (positive control), 200 µg/mL LDSK50-EA, and 200 µg/mL LDSK50-H2O. Cells were stained with ethidium bromide and taken by fluorescence microscope at medium (200×) and high (400×) magnification. LDSK50-EA: fractions of 50% ethanol extract of the L. domesticum skin followed by ethyl acetate (EA) fractionation; LDSK50-H2O: the aqueous phase product when the L. domesticum skin was extracted with 50% aqueous ethanol and partitioned with EA.
Mentions: Fluorescence images of comet TK6 cells evaluated for the different treatment groups were demonstrated in Figure 5.

Bottom Line: The comet results revealed DNA-protective activity of both LDSK50-EA and LDSK50-H2O fractions when TK6 human lymphoblast cells were pre-treated at 25, 50, 100, and 200 μg/mL for 24 h prior to H2O2 exposure.This study generates new information on the biological activity of L. domesticum.It will promote and strengthen the utilization of L. domesticum by-products.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmaceuticals and Natural Products, Thailand Institute of Scientific and Technological Research (TISTR), 35 Mu 3 Techno Polis, Khlong Luang, Pathum Thani 12120, Thailand. prapaipat@tistr.or.th.

ABSTRACT
Lansium domesticum Corr. or "long-kong" is one of the most popular fruits in Thailand. Its peel (skin, SK) and seeds (SD) become waste unless recycled or applied for use. This study was undertaken to determine the bioactivity and phytochemical components of L. domesticum (LD) skin and seed extracts. Following various extraction and fractionation procedures, 12 fractions were obtained. All fractions were tested for antioxidant capacity against O2(-•) and OH(•). It was found that the peel of L. domesticum fruits exhibited higher O2(-•) and OH(•) scavenging activity than seeds. High potential antioxidant activity was found in two fractions of 50% ethanol extract of peel followed by ethyl acetate (EA) fractionation (LDSK50-EA) and its aqueous phase (LDSK50-H2O). Therefore, these two active fractions were selected for further studies on their antioxidative activity against DNA damage by hydrogen peroxide (H2O2) in human TK6 cells using comet assay. The comet results revealed DNA-protective activity of both LDSK50-EA and LDSK50-H2O fractions when TK6 human lymphoblast cells were pre-treated at 25, 50, 100, and 200 μg/mL for 24 h prior to H2O2 exposure. The phytochemical analysis illustrated the presence of phenolic substances, mainly scopoletin, rutin, and chlorogenic acid, in these two active fractions. This study generates new information on the biological activity of L. domesticum. It will promote and strengthen the utilization of L. domesticum by-products.

No MeSH data available.


Related in: MedlinePlus