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Determination of Free Radical Scavenging, Antioxidative DNA Damage Activities and Phytochemical Components of Active Fractions from Lansium domesticum Corr. Fruit.

Klungsupya P, Suthepakul N, Muangman T, Rerk-Am U, Thongdon-A J - Nutrients (2015)

Bottom Line: The comet results revealed DNA-protective activity of both LDSK50-EA and LDSK50-H2O fractions when TK6 human lymphoblast cells were pre-treated at 25, 50, 100, and 200 μg/mL for 24 h prior to H2O2 exposure.This study generates new information on the biological activity of L. domesticum.It will promote and strengthen the utilization of L. domesticum by-products.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmaceuticals and Natural Products, Thailand Institute of Scientific and Technological Research (TISTR), 35 Mu 3 Techno Polis, Khlong Luang, Pathum Thani 12120, Thailand. prapaipat@tistr.or.th.

ABSTRACT
Lansium domesticum Corr. or "long-kong" is one of the most popular fruits in Thailand. Its peel (skin, SK) and seeds (SD) become waste unless recycled or applied for use. This study was undertaken to determine the bioactivity and phytochemical components of L. domesticum (LD) skin and seed extracts. Following various extraction and fractionation procedures, 12 fractions were obtained. All fractions were tested for antioxidant capacity against O2(-•) and OH(•). It was found that the peel of L. domesticum fruits exhibited higher O2(-•) and OH(•) scavenging activity than seeds. High potential antioxidant activity was found in two fractions of 50% ethanol extract of peel followed by ethyl acetate (EA) fractionation (LDSK50-EA) and its aqueous phase (LDSK50-H2O). Therefore, these two active fractions were selected for further studies on their antioxidative activity against DNA damage by hydrogen peroxide (H2O2) in human TK6 cells using comet assay. The comet results revealed DNA-protective activity of both LDSK50-EA and LDSK50-H2O fractions when TK6 human lymphoblast cells were pre-treated at 25, 50, 100, and 200 μg/mL for 24 h prior to H2O2 exposure. The phytochemical analysis illustrated the presence of phenolic substances, mainly scopoletin, rutin, and chlorogenic acid, in these two active fractions. This study generates new information on the biological activity of L. domesticum. It will promote and strengthen the utilization of L. domesticum by-products.

No MeSH data available.


Related in: MedlinePlus

Tail lenght (TL, µm) values measured in pre-treated TK6 cells with LDSK50-EA (fractions of 50% ethanol extract of the L. domesticum skin followed by ethyl acetate (EA) fractionation) and LDSK50-H2O (the aqueous phase product when the L. domesticum skin was extracted with 50% aqueous ethanol and partitioned with EA) fractions followed by H2O2 damage induction by comet assay. Results were expressed as means ± standard deviation (SD) (n = 3). * Significant difference was detected from 50 µM H2O2 treatment groups at p ≤ 0.05.
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nutrients-07-05312-f002: Tail lenght (TL, µm) values measured in pre-treated TK6 cells with LDSK50-EA (fractions of 50% ethanol extract of the L. domesticum skin followed by ethyl acetate (EA) fractionation) and LDSK50-H2O (the aqueous phase product when the L. domesticum skin was extracted with 50% aqueous ethanol and partitioned with EA) fractions followed by H2O2 damage induction by comet assay. Results were expressed as means ± standard deviation (SD) (n = 3). * Significant difference was detected from 50 µM H2O2 treatment groups at p ≤ 0.05.

Mentions: Results detected by comet or SCGE assay revealed that treatment of 50µM H2O2 for 5 min produced DNA damage (% TM, Figure 3) in TK6 cells at about 10-fold greater than untreated cells. Interestingly, this DNA damage could be prevented by pre-treating the TK6 cells with LDSK50-EA at 25, 50, 100, and 200 µg/mL for 24 h. The effect was found to be in a dose-dependent manner. The highest DNA preventive effect was found at 200 µg/mL concentration. In contrast, the LDSK50-H2O fraction exhibited a slight inhibitory effect on oxidative DNA damage when tested at similar concentration ranges. The DNA protective effect against H2O2 of LDSK50-H2O was indicated by a reduction in TL (Figure 2) and TM (= distance between the centre of gravity of the head to the centre of gravity of the tail) × (tail DNA intensity/total comet DNA intensity) (Figure 3) damage parameters in comparison to cells treated with H2O2 alone.


Determination of Free Radical Scavenging, Antioxidative DNA Damage Activities and Phytochemical Components of Active Fractions from Lansium domesticum Corr. Fruit.

Klungsupya P, Suthepakul N, Muangman T, Rerk-Am U, Thongdon-A J - Nutrients (2015)

Tail lenght (TL, µm) values measured in pre-treated TK6 cells with LDSK50-EA (fractions of 50% ethanol extract of the L. domesticum skin followed by ethyl acetate (EA) fractionation) and LDSK50-H2O (the aqueous phase product when the L. domesticum skin was extracted with 50% aqueous ethanol and partitioned with EA) fractions followed by H2O2 damage induction by comet assay. Results were expressed as means ± standard deviation (SD) (n = 3). * Significant difference was detected from 50 µM H2O2 treatment groups at p ≤ 0.05.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4555151&req=5

nutrients-07-05312-f002: Tail lenght (TL, µm) values measured in pre-treated TK6 cells with LDSK50-EA (fractions of 50% ethanol extract of the L. domesticum skin followed by ethyl acetate (EA) fractionation) and LDSK50-H2O (the aqueous phase product when the L. domesticum skin was extracted with 50% aqueous ethanol and partitioned with EA) fractions followed by H2O2 damage induction by comet assay. Results were expressed as means ± standard deviation (SD) (n = 3). * Significant difference was detected from 50 µM H2O2 treatment groups at p ≤ 0.05.
Mentions: Results detected by comet or SCGE assay revealed that treatment of 50µM H2O2 for 5 min produced DNA damage (% TM, Figure 3) in TK6 cells at about 10-fold greater than untreated cells. Interestingly, this DNA damage could be prevented by pre-treating the TK6 cells with LDSK50-EA at 25, 50, 100, and 200 µg/mL for 24 h. The effect was found to be in a dose-dependent manner. The highest DNA preventive effect was found at 200 µg/mL concentration. In contrast, the LDSK50-H2O fraction exhibited a slight inhibitory effect on oxidative DNA damage when tested at similar concentration ranges. The DNA protective effect against H2O2 of LDSK50-H2O was indicated by a reduction in TL (Figure 2) and TM (= distance between the centre of gravity of the head to the centre of gravity of the tail) × (tail DNA intensity/total comet DNA intensity) (Figure 3) damage parameters in comparison to cells treated with H2O2 alone.

Bottom Line: The comet results revealed DNA-protective activity of both LDSK50-EA and LDSK50-H2O fractions when TK6 human lymphoblast cells were pre-treated at 25, 50, 100, and 200 μg/mL for 24 h prior to H2O2 exposure.This study generates new information on the biological activity of L. domesticum.It will promote and strengthen the utilization of L. domesticum by-products.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmaceuticals and Natural Products, Thailand Institute of Scientific and Technological Research (TISTR), 35 Mu 3 Techno Polis, Khlong Luang, Pathum Thani 12120, Thailand. prapaipat@tistr.or.th.

ABSTRACT
Lansium domesticum Corr. or "long-kong" is one of the most popular fruits in Thailand. Its peel (skin, SK) and seeds (SD) become waste unless recycled or applied for use. This study was undertaken to determine the bioactivity and phytochemical components of L. domesticum (LD) skin and seed extracts. Following various extraction and fractionation procedures, 12 fractions were obtained. All fractions were tested for antioxidant capacity against O2(-•) and OH(•). It was found that the peel of L. domesticum fruits exhibited higher O2(-•) and OH(•) scavenging activity than seeds. High potential antioxidant activity was found in two fractions of 50% ethanol extract of peel followed by ethyl acetate (EA) fractionation (LDSK50-EA) and its aqueous phase (LDSK50-H2O). Therefore, these two active fractions were selected for further studies on their antioxidative activity against DNA damage by hydrogen peroxide (H2O2) in human TK6 cells using comet assay. The comet results revealed DNA-protective activity of both LDSK50-EA and LDSK50-H2O fractions when TK6 human lymphoblast cells were pre-treated at 25, 50, 100, and 200 μg/mL for 24 h prior to H2O2 exposure. The phytochemical analysis illustrated the presence of phenolic substances, mainly scopoletin, rutin, and chlorogenic acid, in these two active fractions. This study generates new information on the biological activity of L. domesticum. It will promote and strengthen the utilization of L. domesticum by-products.

No MeSH data available.


Related in: MedlinePlus