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Determination of Free Radical Scavenging, Antioxidative DNA Damage Activities and Phytochemical Components of Active Fractions from Lansium domesticum Corr. Fruit.

Klungsupya P, Suthepakul N, Muangman T, Rerk-Am U, Thongdon-A J - Nutrients (2015)

Bottom Line: The comet results revealed DNA-protective activity of both LDSK50-EA and LDSK50-H2O fractions when TK6 human lymphoblast cells were pre-treated at 25, 50, 100, and 200 μg/mL for 24 h prior to H2O2 exposure.This study generates new information on the biological activity of L. domesticum.It will promote and strengthen the utilization of L. domesticum by-products.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmaceuticals and Natural Products, Thailand Institute of Scientific and Technological Research (TISTR), 35 Mu 3 Techno Polis, Khlong Luang, Pathum Thani 12120, Thailand. prapaipat@tistr.or.th.

ABSTRACT
Lansium domesticum Corr. or "long-kong" is one of the most popular fruits in Thailand. Its peel (skin, SK) and seeds (SD) become waste unless recycled or applied for use. This study was undertaken to determine the bioactivity and phytochemical components of L. domesticum (LD) skin and seed extracts. Following various extraction and fractionation procedures, 12 fractions were obtained. All fractions were tested for antioxidant capacity against O2(-•) and OH(•). It was found that the peel of L. domesticum fruits exhibited higher O2(-•) and OH(•) scavenging activity than seeds. High potential antioxidant activity was found in two fractions of 50% ethanol extract of peel followed by ethyl acetate (EA) fractionation (LDSK50-EA) and its aqueous phase (LDSK50-H2O). Therefore, these two active fractions were selected for further studies on their antioxidative activity against DNA damage by hydrogen peroxide (H2O2) in human TK6 cells using comet assay. The comet results revealed DNA-protective activity of both LDSK50-EA and LDSK50-H2O fractions when TK6 human lymphoblast cells were pre-treated at 25, 50, 100, and 200 μg/mL for 24 h prior to H2O2 exposure. The phytochemical analysis illustrated the presence of phenolic substances, mainly scopoletin, rutin, and chlorogenic acid, in these two active fractions. This study generates new information on the biological activity of L. domesticum. It will promote and strengthen the utilization of L. domesticum by-products.

No MeSH data available.


Related in: MedlinePlus

Illustration of fresh fruit, peel, and seeds of long-kong.
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nutrients-07-05312-f001: Illustration of fresh fruit, peel, and seeds of long-kong.

Mentions: Thailand has a variety of fruits; however, only some of them are widely consumed. Among these is the fruit of Lansium domesticum Corr. which is known in Thai as “long-kong”. It has been very popular in Thailand and surrounding countries in Southeast Asia. It belongs to the Meliaceae family and is known by numerous common names. In Indonesia, it is known mainly as langsat, duku, or kokosan while in Malaysia it is known as langsat, lansa, langseh, or langsep. In the Philippines, it is known as lansones and it is known as bòn-bon in Vietnam [1,2]. The well-known and economic fruit long-kong is largely cultivated in peninsular Thailand, especially in the southern region. Long-kong develops between 15 and 25 fruits per bunch with little non-sticky sap on the skin. The appearance of long-kong fruit is globular in shape with an average size of 1.2–2.4 inches in diameter (Figure 1). It has a brittle and rough skin. It is almost seedless, with five segments of white translucent flesh [3].


Determination of Free Radical Scavenging, Antioxidative DNA Damage Activities and Phytochemical Components of Active Fractions from Lansium domesticum Corr. Fruit.

Klungsupya P, Suthepakul N, Muangman T, Rerk-Am U, Thongdon-A J - Nutrients (2015)

Illustration of fresh fruit, peel, and seeds of long-kong.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4555151&req=5

nutrients-07-05312-f001: Illustration of fresh fruit, peel, and seeds of long-kong.
Mentions: Thailand has a variety of fruits; however, only some of them are widely consumed. Among these is the fruit of Lansium domesticum Corr. which is known in Thai as “long-kong”. It has been very popular in Thailand and surrounding countries in Southeast Asia. It belongs to the Meliaceae family and is known by numerous common names. In Indonesia, it is known mainly as langsat, duku, or kokosan while in Malaysia it is known as langsat, lansa, langseh, or langsep. In the Philippines, it is known as lansones and it is known as bòn-bon in Vietnam [1,2]. The well-known and economic fruit long-kong is largely cultivated in peninsular Thailand, especially in the southern region. Long-kong develops between 15 and 25 fruits per bunch with little non-sticky sap on the skin. The appearance of long-kong fruit is globular in shape with an average size of 1.2–2.4 inches in diameter (Figure 1). It has a brittle and rough skin. It is almost seedless, with five segments of white translucent flesh [3].

Bottom Line: The comet results revealed DNA-protective activity of both LDSK50-EA and LDSK50-H2O fractions when TK6 human lymphoblast cells were pre-treated at 25, 50, 100, and 200 μg/mL for 24 h prior to H2O2 exposure.This study generates new information on the biological activity of L. domesticum.It will promote and strengthen the utilization of L. domesticum by-products.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmaceuticals and Natural Products, Thailand Institute of Scientific and Technological Research (TISTR), 35 Mu 3 Techno Polis, Khlong Luang, Pathum Thani 12120, Thailand. prapaipat@tistr.or.th.

ABSTRACT
Lansium domesticum Corr. or "long-kong" is one of the most popular fruits in Thailand. Its peel (skin, SK) and seeds (SD) become waste unless recycled or applied for use. This study was undertaken to determine the bioactivity and phytochemical components of L. domesticum (LD) skin and seed extracts. Following various extraction and fractionation procedures, 12 fractions were obtained. All fractions were tested for antioxidant capacity against O2(-•) and OH(•). It was found that the peel of L. domesticum fruits exhibited higher O2(-•) and OH(•) scavenging activity than seeds. High potential antioxidant activity was found in two fractions of 50% ethanol extract of peel followed by ethyl acetate (EA) fractionation (LDSK50-EA) and its aqueous phase (LDSK50-H2O). Therefore, these two active fractions were selected for further studies on their antioxidative activity against DNA damage by hydrogen peroxide (H2O2) in human TK6 cells using comet assay. The comet results revealed DNA-protective activity of both LDSK50-EA and LDSK50-H2O fractions when TK6 human lymphoblast cells were pre-treated at 25, 50, 100, and 200 μg/mL for 24 h prior to H2O2 exposure. The phytochemical analysis illustrated the presence of phenolic substances, mainly scopoletin, rutin, and chlorogenic acid, in these two active fractions. This study generates new information on the biological activity of L. domesticum. It will promote and strengthen the utilization of L. domesticum by-products.

No MeSH data available.


Related in: MedlinePlus