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Dietary Selenium Levels Affect Selenoprotein Expression and Support the Interferon-γ and IL-6 Immune Response Pathways in Mice.

Tsuji PA, Carlson BA, Anderson CB, Seifried HE, Hatfield DL, Howard MT - Nutrients (2015)

Bottom Line: Expression levels and translation of mRNAs encoding stress-related selenoproteins were shown to be up-regulated by increased selenium status, as were genes involved in inflammation and response to interferon-γ.Finally, microarray and qPCR analysis of lung tissue demonstrated that the selenium effects on immune function are not limited to liver.These data are consistent with previous reports indicating that adequate selenium levels can support beneficial immune responses, and further identify the IL-6 and interferon-γ pathways as being responsive to dietary selenium intake.

View Article: PubMed Central - PubMed

Affiliation: Department of Biological Sciences, Towson University, Towson, MD, 21252, USA. ptsuji@towson.edu.

ABSTRACT
Selenium is an essential element that is required to support a number of cellular functions and biochemical pathways. The objective of this study was to examine the effects of reduced dietary selenium levels on gene expression to assess changes in expression of non-selenoprotein genes that may contribute to the physiological consequences of selenium deficiency. Mice were fed diets that were either deficient in selenium or supplemented with selenium in the form of sodium selenite for six weeks. Differences in liver mRNA expression and translation were measured using a combination of ribosome profiling, RNA-Seq, microarrays, and qPCR. Expression levels and translation of mRNAs encoding stress-related selenoproteins were shown to be up-regulated by increased selenium status, as were genes involved in inflammation and response to interferon-γ. Changes in serum cytokine levels were measured which confirmed that interferon-γ, as well as IL-6, were increased in selenium adequate mice. Finally, microarray and qPCR analysis of lung tissue demonstrated that the selenium effects on immune function are not limited to liver. These data are consistent with previous reports indicating that adequate selenium levels can support beneficial immune responses, and further identify the IL-6 and interferon-γ pathways as being responsive to dietary selenium intake.

No MeSH data available.


Related in: MedlinePlus

Analysis of interferon-γ-regulated pro-inflammatory mRNA expression in liver by qPCR. Liver mRNA levels for the indicated genes were measured by qPCR for mice fed 0.1 ppm and 0 ppm Se supplemented diets (n = 5). mRNA levels for each gene were normalized to GAPDH, and graphed with box (median plus 75th and 25th percentile) and whisker (minimum and maximum points) plots. (* p < 0.05 compared to 0 Se).
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nutrients-07-05297-f003: Analysis of interferon-γ-regulated pro-inflammatory mRNA expression in liver by qPCR. Liver mRNA levels for the indicated genes were measured by qPCR for mice fed 0.1 ppm and 0 ppm Se supplemented diets (n = 5). mRNA levels for each gene were normalized to GAPDH, and graphed with box (median plus 75th and 25th percentile) and whisker (minimum and maximum points) plots. (* p < 0.05 compared to 0 Se).

Mentions: Many of these cytokine responsive gene changes were subsequently validated with real-time quantitative reverse-transcriptase PCR (qPCR). Gbp1, 2, 6, 7 and 8 were increased in both liver and lung tissues of mice on the Se-adequate diet as shown in Figure 3 and Figure 4, and statistical significance was observed for most, as indicated. Other interferon-γ/Stat-1-regulated genes, such as Irgm2 (p = 0.06), Igtp (p < 0.05), and Tgtp1 (p < 0.05), were also increased in lung tissues of mice on Se-adequate diets.


Dietary Selenium Levels Affect Selenoprotein Expression and Support the Interferon-γ and IL-6 Immune Response Pathways in Mice.

Tsuji PA, Carlson BA, Anderson CB, Seifried HE, Hatfield DL, Howard MT - Nutrients (2015)

Analysis of interferon-γ-regulated pro-inflammatory mRNA expression in liver by qPCR. Liver mRNA levels for the indicated genes were measured by qPCR for mice fed 0.1 ppm and 0 ppm Se supplemented diets (n = 5). mRNA levels for each gene were normalized to GAPDH, and graphed with box (median plus 75th and 25th percentile) and whisker (minimum and maximum points) plots. (* p < 0.05 compared to 0 Se).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4555136&req=5

nutrients-07-05297-f003: Analysis of interferon-γ-regulated pro-inflammatory mRNA expression in liver by qPCR. Liver mRNA levels for the indicated genes were measured by qPCR for mice fed 0.1 ppm and 0 ppm Se supplemented diets (n = 5). mRNA levels for each gene were normalized to GAPDH, and graphed with box (median plus 75th and 25th percentile) and whisker (minimum and maximum points) plots. (* p < 0.05 compared to 0 Se).
Mentions: Many of these cytokine responsive gene changes were subsequently validated with real-time quantitative reverse-transcriptase PCR (qPCR). Gbp1, 2, 6, 7 and 8 were increased in both liver and lung tissues of mice on the Se-adequate diet as shown in Figure 3 and Figure 4, and statistical significance was observed for most, as indicated. Other interferon-γ/Stat-1-regulated genes, such as Irgm2 (p = 0.06), Igtp (p < 0.05), and Tgtp1 (p < 0.05), were also increased in lung tissues of mice on Se-adequate diets.

Bottom Line: Expression levels and translation of mRNAs encoding stress-related selenoproteins were shown to be up-regulated by increased selenium status, as were genes involved in inflammation and response to interferon-γ.Finally, microarray and qPCR analysis of lung tissue demonstrated that the selenium effects on immune function are not limited to liver.These data are consistent with previous reports indicating that adequate selenium levels can support beneficial immune responses, and further identify the IL-6 and interferon-γ pathways as being responsive to dietary selenium intake.

View Article: PubMed Central - PubMed

Affiliation: Department of Biological Sciences, Towson University, Towson, MD, 21252, USA. ptsuji@towson.edu.

ABSTRACT
Selenium is an essential element that is required to support a number of cellular functions and biochemical pathways. The objective of this study was to examine the effects of reduced dietary selenium levels on gene expression to assess changes in expression of non-selenoprotein genes that may contribute to the physiological consequences of selenium deficiency. Mice were fed diets that were either deficient in selenium or supplemented with selenium in the form of sodium selenite for six weeks. Differences in liver mRNA expression and translation were measured using a combination of ribosome profiling, RNA-Seq, microarrays, and qPCR. Expression levels and translation of mRNAs encoding stress-related selenoproteins were shown to be up-regulated by increased selenium status, as were genes involved in inflammation and response to interferon-γ. Changes in serum cytokine levels were measured which confirmed that interferon-γ, as well as IL-6, were increased in selenium adequate mice. Finally, microarray and qPCR analysis of lung tissue demonstrated that the selenium effects on immune function are not limited to liver. These data are consistent with previous reports indicating that adequate selenium levels can support beneficial immune responses, and further identify the IL-6 and interferon-γ pathways as being responsive to dietary selenium intake.

No MeSH data available.


Related in: MedlinePlus