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Rice Bran Protein Hydrolysates Improve Insulin Resistance and Decrease Pro-inflammatory Cytokine Gene Expression in Rats Fed a High Carbohydrate-High Fat Diet.

Boonloh K, Kukongviriyapan V, Kongyingyoes B, Kukongviriyapan U, Thawornchinsombut S, Pannangpetch P - Nutrients (2015)

Bottom Line: In the present study, we investigated the beneficial effects of rice bran protein hydrolysates (RBP) in HCHF-induced MS rats.Expression of Ppar-γ mRNA in adipose tissues was significantly increased whereas expression of lipogenic genes Srebf1 and Fasn was significantly decreased.Levels of mRNA of proinflammatory cytokines, Il-6, Tnf-α, Nos-2 and Mcp-1 were significantly decreased.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacology, Faculty of Medicine, Khon Kaen University, 123 Mittraparp Highway, Muang District, Khon Kaen 40002, Thailand. kampeeporn@yahoo.com.

ABSTRACT
A high carbohydrate-high fat (HCHF) diet causes insulin resistance (IR) and metabolic syndrome (MS). Rice bran has been demonstrated to have anti-dyslipidemic and anti-atherogenic properties in an obese mouse model. In the present study, we investigated the beneficial effects of rice bran protein hydrolysates (RBP) in HCHF-induced MS rats. After 12 weeks on this diet, the HCHF-fed group was divided into four subgroups, which were orally administered RBP 100 or 500 mg/kg, pioglitazone 10 mg/kg, or tap water for a further 6 weeks. Compared with normal diet control group, the MS rats had elevated levels of blood glucose, lipid, insulin, and HOMA-IR. Treatment with RBP significantly alleviated all those changes and restored insulin sensitivity. Additionally, RBP treatment increased adiponectin and suppressed leptin levels. Expression of Ppar-γ mRNA in adipose tissues was significantly increased whereas expression of lipogenic genes Srebf1 and Fasn was significantly decreased. Levels of mRNA of proinflammatory cytokines, Il-6, Tnf-α, Nos-2 and Mcp-1 were significantly decreased. In conclusion, the present findings support the consumption of RBP as a functional food to improve insulin resistance and to prevent the development of metabolic syndrome.

No MeSH data available.


Related in: MedlinePlus

Effects of RBP on expression levels of inflammatory genes Il-6 (A); Tnf-α (B); Mcp-1 (C); Nos2 (D) and Il-10 (E) in HCHF-fed rats. Oral administration of RBP 500 mg/kg or pioglitazone 10 mg/kg daily for 6 weeks significantly decreased the expression of Il-6, Tnf-α, Mcp-1 and Nos2. RBP 500 tended to increase expression of the anti-inflammatory gene Il-10 as compared to the HCHF-control group (*: p < 0.05, significant increase as compared to normal controls; #: p < 0.05, significant decrease as compared to HCHF-control group).
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nutrients-07-05292-f006: Effects of RBP on expression levels of inflammatory genes Il-6 (A); Tnf-α (B); Mcp-1 (C); Nos2 (D) and Il-10 (E) in HCHF-fed rats. Oral administration of RBP 500 mg/kg or pioglitazone 10 mg/kg daily for 6 weeks significantly decreased the expression of Il-6, Tnf-α, Mcp-1 and Nos2. RBP 500 tended to increase expression of the anti-inflammatory gene Il-10 as compared to the HCHF-control group (*: p < 0.05, significant increase as compared to normal controls; #: p < 0.05, significant decrease as compared to HCHF-control group).

Mentions: Low-grade chronic inflammation is related to insulin resistance in metabolic syndrome. Thus, we investigated the effects of RBP on the expression levels of proinflammatory and anti-inflammatory genes in intra-abdominal fat cells. Adipose tissues from the HCHF-control group showed a significant increase in the mRNA levels of Il-6, Tnf-α, Mcp-1 and Nos2 (Figure 6). RBP and pioglitazone significantly decreased the expression of all proinflammatory genes (Figure 6A–D). On the other hand, the expression of the anti-inflammatory gene Il-10 was significant decreased in the HCHF-control group. Administration of RBP or pioglitazone produced an increase in the expression of Il-10 (Figure 6E). Together, these results suggest that RBP may improve insulin resistance via, at least in part, decreasing inflammatory cytokine induced-insulin resistance in HCHF-fed rats.


Rice Bran Protein Hydrolysates Improve Insulin Resistance and Decrease Pro-inflammatory Cytokine Gene Expression in Rats Fed a High Carbohydrate-High Fat Diet.

Boonloh K, Kukongviriyapan V, Kongyingyoes B, Kukongviriyapan U, Thawornchinsombut S, Pannangpetch P - Nutrients (2015)

Effects of RBP on expression levels of inflammatory genes Il-6 (A); Tnf-α (B); Mcp-1 (C); Nos2 (D) and Il-10 (E) in HCHF-fed rats. Oral administration of RBP 500 mg/kg or pioglitazone 10 mg/kg daily for 6 weeks significantly decreased the expression of Il-6, Tnf-α, Mcp-1 and Nos2. RBP 500 tended to increase expression of the anti-inflammatory gene Il-10 as compared to the HCHF-control group (*: p < 0.05, significant increase as compared to normal controls; #: p < 0.05, significant decrease as compared to HCHF-control group).
© Copyright Policy
Related In: Results  -  Collection

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Show All Figures
getmorefigures.php?uid=PMC4555131&req=5

nutrients-07-05292-f006: Effects of RBP on expression levels of inflammatory genes Il-6 (A); Tnf-α (B); Mcp-1 (C); Nos2 (D) and Il-10 (E) in HCHF-fed rats. Oral administration of RBP 500 mg/kg or pioglitazone 10 mg/kg daily for 6 weeks significantly decreased the expression of Il-6, Tnf-α, Mcp-1 and Nos2. RBP 500 tended to increase expression of the anti-inflammatory gene Il-10 as compared to the HCHF-control group (*: p < 0.05, significant increase as compared to normal controls; #: p < 0.05, significant decrease as compared to HCHF-control group).
Mentions: Low-grade chronic inflammation is related to insulin resistance in metabolic syndrome. Thus, we investigated the effects of RBP on the expression levels of proinflammatory and anti-inflammatory genes in intra-abdominal fat cells. Adipose tissues from the HCHF-control group showed a significant increase in the mRNA levels of Il-6, Tnf-α, Mcp-1 and Nos2 (Figure 6). RBP and pioglitazone significantly decreased the expression of all proinflammatory genes (Figure 6A–D). On the other hand, the expression of the anti-inflammatory gene Il-10 was significant decreased in the HCHF-control group. Administration of RBP or pioglitazone produced an increase in the expression of Il-10 (Figure 6E). Together, these results suggest that RBP may improve insulin resistance via, at least in part, decreasing inflammatory cytokine induced-insulin resistance in HCHF-fed rats.

Bottom Line: In the present study, we investigated the beneficial effects of rice bran protein hydrolysates (RBP) in HCHF-induced MS rats.Expression of Ppar-γ mRNA in adipose tissues was significantly increased whereas expression of lipogenic genes Srebf1 and Fasn was significantly decreased.Levels of mRNA of proinflammatory cytokines, Il-6, Tnf-α, Nos-2 and Mcp-1 were significantly decreased.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacology, Faculty of Medicine, Khon Kaen University, 123 Mittraparp Highway, Muang District, Khon Kaen 40002, Thailand. kampeeporn@yahoo.com.

ABSTRACT
A high carbohydrate-high fat (HCHF) diet causes insulin resistance (IR) and metabolic syndrome (MS). Rice bran has been demonstrated to have anti-dyslipidemic and anti-atherogenic properties in an obese mouse model. In the present study, we investigated the beneficial effects of rice bran protein hydrolysates (RBP) in HCHF-induced MS rats. After 12 weeks on this diet, the HCHF-fed group was divided into four subgroups, which were orally administered RBP 100 or 500 mg/kg, pioglitazone 10 mg/kg, or tap water for a further 6 weeks. Compared with normal diet control group, the MS rats had elevated levels of blood glucose, lipid, insulin, and HOMA-IR. Treatment with RBP significantly alleviated all those changes and restored insulin sensitivity. Additionally, RBP treatment increased adiponectin and suppressed leptin levels. Expression of Ppar-γ mRNA in adipose tissues was significantly increased whereas expression of lipogenic genes Srebf1 and Fasn was significantly decreased. Levels of mRNA of proinflammatory cytokines, Il-6, Tnf-α, Nos-2 and Mcp-1 were significantly decreased. In conclusion, the present findings support the consumption of RBP as a functional food to improve insulin resistance and to prevent the development of metabolic syndrome.

No MeSH data available.


Related in: MedlinePlus