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Rice Bran Protein Hydrolysates Improve Insulin Resistance and Decrease Pro-inflammatory Cytokine Gene Expression in Rats Fed a High Carbohydrate-High Fat Diet.

Boonloh K, Kukongviriyapan V, Kongyingyoes B, Kukongviriyapan U, Thawornchinsombut S, Pannangpetch P - Nutrients (2015)

Bottom Line: In the present study, we investigated the beneficial effects of rice bran protein hydrolysates (RBP) in HCHF-induced MS rats.Expression of Ppar-γ mRNA in adipose tissues was significantly increased whereas expression of lipogenic genes Srebf1 and Fasn was significantly decreased.Levels of mRNA of proinflammatory cytokines, Il-6, Tnf-α, Nos-2 and Mcp-1 were significantly decreased.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacology, Faculty of Medicine, Khon Kaen University, 123 Mittraparp Highway, Muang District, Khon Kaen 40002, Thailand. kampeeporn@yahoo.com.

ABSTRACT
A high carbohydrate-high fat (HCHF) diet causes insulin resistance (IR) and metabolic syndrome (MS). Rice bran has been demonstrated to have anti-dyslipidemic and anti-atherogenic properties in an obese mouse model. In the present study, we investigated the beneficial effects of rice bran protein hydrolysates (RBP) in HCHF-induced MS rats. After 12 weeks on this diet, the HCHF-fed group was divided into four subgroups, which were orally administered RBP 100 or 500 mg/kg, pioglitazone 10 mg/kg, or tap water for a further 6 weeks. Compared with normal diet control group, the MS rats had elevated levels of blood glucose, lipid, insulin, and HOMA-IR. Treatment with RBP significantly alleviated all those changes and restored insulin sensitivity. Additionally, RBP treatment increased adiponectin and suppressed leptin levels. Expression of Ppar-γ mRNA in adipose tissues was significantly increased whereas expression of lipogenic genes Srebf1 and Fasn was significantly decreased. Levels of mRNA of proinflammatory cytokines, Il-6, Tnf-α, Nos-2 and Mcp-1 were significantly decreased. In conclusion, the present findings support the consumption of RBP as a functional food to improve insulin resistance and to prevent the development of metabolic syndrome.

No MeSH data available.


Related in: MedlinePlus

Effects of RBP on expression of lipogenic genes Srebf1 (A) and Fasn (B) in HCHF-fed rats. Oral administration of RBP 500 mg/kg or pioglitazone 10 mg/kg daily for 6 weeks significantly decreased the expression of Srebf1 and Fasn. (*: p < 0.05, significant increase as compared to normal controls; #: p < 0.05, significant decrease as compared to HCHF-control group).
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nutrients-07-05292-f005: Effects of RBP on expression of lipogenic genes Srebf1 (A) and Fasn (B) in HCHF-fed rats. Oral administration of RBP 500 mg/kg or pioglitazone 10 mg/kg daily for 6 weeks significantly decreased the expression of Srebf1 and Fasn. (*: p < 0.05, significant increase as compared to normal controls; #: p < 0.05, significant decrease as compared to HCHF-control group).

Mentions: To gain insight into the effect of RBP on lipid metabolism, we investigated the alteration in expression of the transcriptional factor involved in de novo lipogenesis in the liver, i.e., Srebf1 and its downstream gene, the fatty acid synthase gene (Fasn), by RT-qPCR. Consistent with high-fat feeding, the expression of Srebf1 was significantly greater in the HCHF-fed rats than in the normal control group. Interestingly, the expression of Srebf1 was significantly decreased in HCHF-fed rats receiving RBP and pioglitazone (Figure 5A). Expression of the downstream gene Fasn was also significantly increased in HCHF-fed rats and partially reversed by RBP 500 mg/kg or pioglitazone treatments (Figure 5B).


Rice Bran Protein Hydrolysates Improve Insulin Resistance and Decrease Pro-inflammatory Cytokine Gene Expression in Rats Fed a High Carbohydrate-High Fat Diet.

Boonloh K, Kukongviriyapan V, Kongyingyoes B, Kukongviriyapan U, Thawornchinsombut S, Pannangpetch P - Nutrients (2015)

Effects of RBP on expression of lipogenic genes Srebf1 (A) and Fasn (B) in HCHF-fed rats. Oral administration of RBP 500 mg/kg or pioglitazone 10 mg/kg daily for 6 weeks significantly decreased the expression of Srebf1 and Fasn. (*: p < 0.05, significant increase as compared to normal controls; #: p < 0.05, significant decrease as compared to HCHF-control group).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4555131&req=5

nutrients-07-05292-f005: Effects of RBP on expression of lipogenic genes Srebf1 (A) and Fasn (B) in HCHF-fed rats. Oral administration of RBP 500 mg/kg or pioglitazone 10 mg/kg daily for 6 weeks significantly decreased the expression of Srebf1 and Fasn. (*: p < 0.05, significant increase as compared to normal controls; #: p < 0.05, significant decrease as compared to HCHF-control group).
Mentions: To gain insight into the effect of RBP on lipid metabolism, we investigated the alteration in expression of the transcriptional factor involved in de novo lipogenesis in the liver, i.e., Srebf1 and its downstream gene, the fatty acid synthase gene (Fasn), by RT-qPCR. Consistent with high-fat feeding, the expression of Srebf1 was significantly greater in the HCHF-fed rats than in the normal control group. Interestingly, the expression of Srebf1 was significantly decreased in HCHF-fed rats receiving RBP and pioglitazone (Figure 5A). Expression of the downstream gene Fasn was also significantly increased in HCHF-fed rats and partially reversed by RBP 500 mg/kg or pioglitazone treatments (Figure 5B).

Bottom Line: In the present study, we investigated the beneficial effects of rice bran protein hydrolysates (RBP) in HCHF-induced MS rats.Expression of Ppar-γ mRNA in adipose tissues was significantly increased whereas expression of lipogenic genes Srebf1 and Fasn was significantly decreased.Levels of mRNA of proinflammatory cytokines, Il-6, Tnf-α, Nos-2 and Mcp-1 were significantly decreased.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacology, Faculty of Medicine, Khon Kaen University, 123 Mittraparp Highway, Muang District, Khon Kaen 40002, Thailand. kampeeporn@yahoo.com.

ABSTRACT
A high carbohydrate-high fat (HCHF) diet causes insulin resistance (IR) and metabolic syndrome (MS). Rice bran has been demonstrated to have anti-dyslipidemic and anti-atherogenic properties in an obese mouse model. In the present study, we investigated the beneficial effects of rice bran protein hydrolysates (RBP) in HCHF-induced MS rats. After 12 weeks on this diet, the HCHF-fed group was divided into four subgroups, which were orally administered RBP 100 or 500 mg/kg, pioglitazone 10 mg/kg, or tap water for a further 6 weeks. Compared with normal diet control group, the MS rats had elevated levels of blood glucose, lipid, insulin, and HOMA-IR. Treatment with RBP significantly alleviated all those changes and restored insulin sensitivity. Additionally, RBP treatment increased adiponectin and suppressed leptin levels. Expression of Ppar-γ mRNA in adipose tissues was significantly increased whereas expression of lipogenic genes Srebf1 and Fasn was significantly decreased. Levels of mRNA of proinflammatory cytokines, Il-6, Tnf-α, Nos-2 and Mcp-1 were significantly decreased. In conclusion, the present findings support the consumption of RBP as a functional food to improve insulin resistance and to prevent the development of metabolic syndrome.

No MeSH data available.


Related in: MedlinePlus