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Rice Bran Protein Hydrolysates Improve Insulin Resistance and Decrease Pro-inflammatory Cytokine Gene Expression in Rats Fed a High Carbohydrate-High Fat Diet.

Boonloh K, Kukongviriyapan V, Kongyingyoes B, Kukongviriyapan U, Thawornchinsombut S, Pannangpetch P - Nutrients (2015)

Bottom Line: In the present study, we investigated the beneficial effects of rice bran protein hydrolysates (RBP) in HCHF-induced MS rats.Expression of Ppar-γ mRNA in adipose tissues was significantly increased whereas expression of lipogenic genes Srebf1 and Fasn was significantly decreased.Levels of mRNA of proinflammatory cytokines, Il-6, Tnf-α, Nos-2 and Mcp-1 were significantly decreased.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacology, Faculty of Medicine, Khon Kaen University, 123 Mittraparp Highway, Muang District, Khon Kaen 40002, Thailand. kampeeporn@yahoo.com.

ABSTRACT
A high carbohydrate-high fat (HCHF) diet causes insulin resistance (IR) and metabolic syndrome (MS). Rice bran has been demonstrated to have anti-dyslipidemic and anti-atherogenic properties in an obese mouse model. In the present study, we investigated the beneficial effects of rice bran protein hydrolysates (RBP) in HCHF-induced MS rats. After 12 weeks on this diet, the HCHF-fed group was divided into four subgroups, which were orally administered RBP 100 or 500 mg/kg, pioglitazone 10 mg/kg, or tap water for a further 6 weeks. Compared with normal diet control group, the MS rats had elevated levels of blood glucose, lipid, insulin, and HOMA-IR. Treatment with RBP significantly alleviated all those changes and restored insulin sensitivity. Additionally, RBP treatment increased adiponectin and suppressed leptin levels. Expression of Ppar-γ mRNA in adipose tissues was significantly increased whereas expression of lipogenic genes Srebf1 and Fasn was significantly decreased. Levels of mRNA of proinflammatory cytokines, Il-6, Tnf-α, Nos-2 and Mcp-1 were significantly decreased. In conclusion, the present findings support the consumption of RBP as a functional food to improve insulin resistance and to prevent the development of metabolic syndrome.

No MeSH data available.


Related in: MedlinePlus

Effects of RBP on expression of adipocyte Ppar-γ gene in HCHF-fed rats. Oral administration of RBP 500 mg/kg or pioglitazone 10 mg/kg daily for 6 weeks significantly increased Ppar-γ expression (†: p < 0.05, significant increase as compared to the HCHF-control group).
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nutrients-07-05292-f004: Effects of RBP on expression of adipocyte Ppar-γ gene in HCHF-fed rats. Oral administration of RBP 500 mg/kg or pioglitazone 10 mg/kg daily for 6 weeks significantly increased Ppar-γ expression (†: p < 0.05, significant increase as compared to the HCHF-control group).

Mentions: PPAR-γ functions to trigger the secretion of adiponectin from adipose tissues. Adiponectin increases fatty acid oxidation in liver and skeletal muscle, and improves insulin sensitivity. We found that expression of Ppar-γ in intra-abdominal fat cells in the HCHF-control group was slightly decreased, but interestingly, was significantly increased in RBP 500 mg/kg or pioglitazone treated groups (Figure 4). These results reveal that RBP may attenuate insulin resistance by enhancing the expression of Ppar-γ.


Rice Bran Protein Hydrolysates Improve Insulin Resistance and Decrease Pro-inflammatory Cytokine Gene Expression in Rats Fed a High Carbohydrate-High Fat Diet.

Boonloh K, Kukongviriyapan V, Kongyingyoes B, Kukongviriyapan U, Thawornchinsombut S, Pannangpetch P - Nutrients (2015)

Effects of RBP on expression of adipocyte Ppar-γ gene in HCHF-fed rats. Oral administration of RBP 500 mg/kg or pioglitazone 10 mg/kg daily for 6 weeks significantly increased Ppar-γ expression (†: p < 0.05, significant increase as compared to the HCHF-control group).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4555131&req=5

nutrients-07-05292-f004: Effects of RBP on expression of adipocyte Ppar-γ gene in HCHF-fed rats. Oral administration of RBP 500 mg/kg or pioglitazone 10 mg/kg daily for 6 weeks significantly increased Ppar-γ expression (†: p < 0.05, significant increase as compared to the HCHF-control group).
Mentions: PPAR-γ functions to trigger the secretion of adiponectin from adipose tissues. Adiponectin increases fatty acid oxidation in liver and skeletal muscle, and improves insulin sensitivity. We found that expression of Ppar-γ in intra-abdominal fat cells in the HCHF-control group was slightly decreased, but interestingly, was significantly increased in RBP 500 mg/kg or pioglitazone treated groups (Figure 4). These results reveal that RBP may attenuate insulin resistance by enhancing the expression of Ppar-γ.

Bottom Line: In the present study, we investigated the beneficial effects of rice bran protein hydrolysates (RBP) in HCHF-induced MS rats.Expression of Ppar-γ mRNA in adipose tissues was significantly increased whereas expression of lipogenic genes Srebf1 and Fasn was significantly decreased.Levels of mRNA of proinflammatory cytokines, Il-6, Tnf-α, Nos-2 and Mcp-1 were significantly decreased.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacology, Faculty of Medicine, Khon Kaen University, 123 Mittraparp Highway, Muang District, Khon Kaen 40002, Thailand. kampeeporn@yahoo.com.

ABSTRACT
A high carbohydrate-high fat (HCHF) diet causes insulin resistance (IR) and metabolic syndrome (MS). Rice bran has been demonstrated to have anti-dyslipidemic and anti-atherogenic properties in an obese mouse model. In the present study, we investigated the beneficial effects of rice bran protein hydrolysates (RBP) in HCHF-induced MS rats. After 12 weeks on this diet, the HCHF-fed group was divided into four subgroups, which were orally administered RBP 100 or 500 mg/kg, pioglitazone 10 mg/kg, or tap water for a further 6 weeks. Compared with normal diet control group, the MS rats had elevated levels of blood glucose, lipid, insulin, and HOMA-IR. Treatment with RBP significantly alleviated all those changes and restored insulin sensitivity. Additionally, RBP treatment increased adiponectin and suppressed leptin levels. Expression of Ppar-γ mRNA in adipose tissues was significantly increased whereas expression of lipogenic genes Srebf1 and Fasn was significantly decreased. Levels of mRNA of proinflammatory cytokines, Il-6, Tnf-α, Nos-2 and Mcp-1 were significantly decreased. In conclusion, the present findings support the consumption of RBP as a functional food to improve insulin resistance and to prevent the development of metabolic syndrome.

No MeSH data available.


Related in: MedlinePlus