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Partial Enteral Nutrition Preserves Elements of Gut Barrier Function, Including Innate Immunity, Intestinal Alkaline Phosphatase (IAP) Level, and Intestinal Microbiota in Mice.

Wan X, Bi J, Gao X, Tian F, Wang X, Li N, Li J - Nutrients (2015)

Bottom Line: However, the effects of partial EN on intestina linnate immunity, intestinal alkaline phosphatase (IAP) and microbiota remain unclear.Administration of 20% EN supplemented with PN significantly increased the concentrations of lysozyme, MUC2, IAP, and the mRNA levels of lysozyme and MUC2 (p < 0.001).The percentages of Bacteroidetes and Tenericutes were significantly lower in the 20% EN group than in the TPN group (p < 0.001).

View Article: PubMed Central - PubMed

Affiliation: Department of General Surgery, Jinling Hospital, School of Medicine, Nanjing University, Nanjing 210002, China. van395412495@sina.cn.

ABSTRACT
Lack of enteral nutrition (EN) during parenteral nutrition (PN) leads to higher incidence of infection because of gut barrier dysfunction. However, the effects of partial EN on intestina linnate immunity, intestinal alkaline phosphatase (IAP) and microbiota remain unclear. The mice were randomized into six groups to receive either standard chow or isocaloric and isonitrogenous nutritional support with variable partial EN to PN ratios. Five days later, the mice were sacrificed and tissue samples were collected. Bacterial translocation, the levels of lysozyme, mucin 2 (MUC2), and IAP were analyzed. The composition of intestinal microbiota was analyzed by 16S rRNA pyrosequencing. Compared with chow, total parenteral nutrition (TPN) resulted in a dysfunctional mucosal barrier, as evidenced by increased bacterial translocation (p < 0.05), loss of lysozyme, MUC2, and IAP, and changes in the gut microbiota (p < 0.001). Administration of 20% EN supplemented with PN significantly increased the concentrations of lysozyme, MUC2, IAP, and the mRNA levels of lysozyme and MUC2 (p < 0.001). The percentages of Bacteroidetes and Tenericutes were significantly lower in the 20% EN group than in the TPN group (p < 0.001). These changes were accompanied by maintained barrier function in bacterial culture (p < 0.05). Supplementation of PN with 20% EN preserves gut barrier function, by way of maintaining innate immunity, IAP and intestinal microbiota.

No MeSH data available.


Related in: MedlinePlus

Regression analysis of bacterial translocation and some other defensive components. (A) Number of goblet cells; (B) Relative dose of lysozyme; (C) Relative dose of intestinal alkaline phosphatase (IAP); (D) Relative dose of mucin 2 (MUC2). n = 36 for each analysis.
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nutrients-07-05288-f008: Regression analysis of bacterial translocation and some other defensive components. (A) Number of goblet cells; (B) Relative dose of lysozyme; (C) Relative dose of intestinal alkaline phosphatase (IAP); (D) Relative dose of mucin 2 (MUC2). n = 36 for each analysis.

Mentions: The relationships between the bacterial culture and other variables were evaluated using regression analysis, and significant correlations are established in terms of number of goblet cell (r2 = 0.791, p < 0.001), relative lysozyme expression (r2 = 0.866, p < 0.001), relative IAP expression (r2 = 0.875, p < 0.001) and relative MUC2 expression (r2 = 0.762, p < 0.001). All the significant results are shown in Figure 8. However, no linear or quadratic effect was observed in regard to the percentage of intestinal microbiota and other components.


Partial Enteral Nutrition Preserves Elements of Gut Barrier Function, Including Innate Immunity, Intestinal Alkaline Phosphatase (IAP) Level, and Intestinal Microbiota in Mice.

Wan X, Bi J, Gao X, Tian F, Wang X, Li N, Li J - Nutrients (2015)

Regression analysis of bacterial translocation and some other defensive components. (A) Number of goblet cells; (B) Relative dose of lysozyme; (C) Relative dose of intestinal alkaline phosphatase (IAP); (D) Relative dose of mucin 2 (MUC2). n = 36 for each analysis.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4555127&req=5

nutrients-07-05288-f008: Regression analysis of bacterial translocation and some other defensive components. (A) Number of goblet cells; (B) Relative dose of lysozyme; (C) Relative dose of intestinal alkaline phosphatase (IAP); (D) Relative dose of mucin 2 (MUC2). n = 36 for each analysis.
Mentions: The relationships between the bacterial culture and other variables were evaluated using regression analysis, and significant correlations are established in terms of number of goblet cell (r2 = 0.791, p < 0.001), relative lysozyme expression (r2 = 0.866, p < 0.001), relative IAP expression (r2 = 0.875, p < 0.001) and relative MUC2 expression (r2 = 0.762, p < 0.001). All the significant results are shown in Figure 8. However, no linear or quadratic effect was observed in regard to the percentage of intestinal microbiota and other components.

Bottom Line: However, the effects of partial EN on intestina linnate immunity, intestinal alkaline phosphatase (IAP) and microbiota remain unclear.Administration of 20% EN supplemented with PN significantly increased the concentrations of lysozyme, MUC2, IAP, and the mRNA levels of lysozyme and MUC2 (p < 0.001).The percentages of Bacteroidetes and Tenericutes were significantly lower in the 20% EN group than in the TPN group (p < 0.001).

View Article: PubMed Central - PubMed

Affiliation: Department of General Surgery, Jinling Hospital, School of Medicine, Nanjing University, Nanjing 210002, China. van395412495@sina.cn.

ABSTRACT
Lack of enteral nutrition (EN) during parenteral nutrition (PN) leads to higher incidence of infection because of gut barrier dysfunction. However, the effects of partial EN on intestina linnate immunity, intestinal alkaline phosphatase (IAP) and microbiota remain unclear. The mice were randomized into six groups to receive either standard chow or isocaloric and isonitrogenous nutritional support with variable partial EN to PN ratios. Five days later, the mice were sacrificed and tissue samples were collected. Bacterial translocation, the levels of lysozyme, mucin 2 (MUC2), and IAP were analyzed. The composition of intestinal microbiota was analyzed by 16S rRNA pyrosequencing. Compared with chow, total parenteral nutrition (TPN) resulted in a dysfunctional mucosal barrier, as evidenced by increased bacterial translocation (p < 0.05), loss of lysozyme, MUC2, and IAP, and changes in the gut microbiota (p < 0.001). Administration of 20% EN supplemented with PN significantly increased the concentrations of lysozyme, MUC2, IAP, and the mRNA levels of lysozyme and MUC2 (p < 0.001). The percentages of Bacteroidetes and Tenericutes were significantly lower in the 20% EN group than in the TPN group (p < 0.001). These changes were accompanied by maintained barrier function in bacterial culture (p < 0.05). Supplementation of PN with 20% EN preserves gut barrier function, by way of maintaining innate immunity, IAP and intestinal microbiota.

No MeSH data available.


Related in: MedlinePlus