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Partial Enteral Nutrition Preserves Elements of Gut Barrier Function, Including Innate Immunity, Intestinal Alkaline Phosphatase (IAP) Level, and Intestinal Microbiota in Mice.

Wan X, Bi J, Gao X, Tian F, Wang X, Li N, Li J - Nutrients (2015)

Bottom Line: However, the effects of partial EN on intestina linnate immunity, intestinal alkaline phosphatase (IAP) and microbiota remain unclear.Administration of 20% EN supplemented with PN significantly increased the concentrations of lysozyme, MUC2, IAP, and the mRNA levels of lysozyme and MUC2 (p < 0.001).The percentages of Bacteroidetes and Tenericutes were significantly lower in the 20% EN group than in the TPN group (p < 0.001).

View Article: PubMed Central - PubMed

Affiliation: Department of General Surgery, Jinling Hospital, School of Medicine, Nanjing University, Nanjing 210002, China. van395412495@sina.cn.

ABSTRACT
Lack of enteral nutrition (EN) during parenteral nutrition (PN) leads to higher incidence of infection because of gut barrier dysfunction. However, the effects of partial EN on intestina linnate immunity, intestinal alkaline phosphatase (IAP) and microbiota remain unclear. The mice were randomized into six groups to receive either standard chow or isocaloric and isonitrogenous nutritional support with variable partial EN to PN ratios. Five days later, the mice were sacrificed and tissue samples were collected. Bacterial translocation, the levels of lysozyme, mucin 2 (MUC2), and IAP were analyzed. The composition of intestinal microbiota was analyzed by 16S rRNA pyrosequencing. Compared with chow, total parenteral nutrition (TPN) resulted in a dysfunctional mucosal barrier, as evidenced by increased bacterial translocation (p < 0.05), loss of lysozyme, MUC2, and IAP, and changes in the gut microbiota (p < 0.001). Administration of 20% EN supplemented with PN significantly increased the concentrations of lysozyme, MUC2, IAP, and the mRNA levels of lysozyme and MUC2 (p < 0.001). The percentages of Bacteroidetes and Tenericutes were significantly lower in the 20% EN group than in the TPN group (p < 0.001). These changes were accompanied by maintained barrier function in bacterial culture (p < 0.05). Supplementation of PN with 20% EN preserves gut barrier function, by way of maintaining innate immunity, IAP and intestinal microbiota.

No MeSH data available.


Related in: MedlinePlus

Reverse transcription quantitative polymerase chain reaction analysis of the expression levels of innate immunity products in the ileal tissue. (A) Lysozyme; (B) mucin 2 (MUC2) and (C) intestinal alkaline phosphatase (IAP). Data are represented as the mean ± standard error of the mean (SEM). *p < 0.05 vs. chow. **p < 0.001 vs. chow. #p < 0.05 vs. TPN. ##p < 0.001 vs. TPN. EN: enteral nutrition; TPN: total parenteral nutrition.
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nutrients-07-05288-f005: Reverse transcription quantitative polymerase chain reaction analysis of the expression levels of innate immunity products in the ileal tissue. (A) Lysozyme; (B) mucin 2 (MUC2) and (C) intestinal alkaline phosphatase (IAP). Data are represented as the mean ± standard error of the mean (SEM). *p < 0.05 vs. chow. **p < 0.001 vs. chow. #p < 0.05 vs. TPN. ##p < 0.001 vs. TPN. EN: enteral nutrition; TPN: total parenteral nutrition.

Mentions: Consistent with the results of the protein-content analysis, qPCR analysis of lysozyme revealed thatrelative expression of lysozymewas significantly lower in the TPN groupcompared with the chow group (p = 0.013). In the 10% EN group, expression of lysozyme mRNA was lower than in the chow group (p < 0.05); however, the levels of lysozyme mRNA expression observed in the 20% EN, 40% EN and 60% EN groups were similar to that in chow groups (Figure 5A and Table S3).


Partial Enteral Nutrition Preserves Elements of Gut Barrier Function, Including Innate Immunity, Intestinal Alkaline Phosphatase (IAP) Level, and Intestinal Microbiota in Mice.

Wan X, Bi J, Gao X, Tian F, Wang X, Li N, Li J - Nutrients (2015)

Reverse transcription quantitative polymerase chain reaction analysis of the expression levels of innate immunity products in the ileal tissue. (A) Lysozyme; (B) mucin 2 (MUC2) and (C) intestinal alkaline phosphatase (IAP). Data are represented as the mean ± standard error of the mean (SEM). *p < 0.05 vs. chow. **p < 0.001 vs. chow. #p < 0.05 vs. TPN. ##p < 0.001 vs. TPN. EN: enteral nutrition; TPN: total parenteral nutrition.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4555127&req=5

nutrients-07-05288-f005: Reverse transcription quantitative polymerase chain reaction analysis of the expression levels of innate immunity products in the ileal tissue. (A) Lysozyme; (B) mucin 2 (MUC2) and (C) intestinal alkaline phosphatase (IAP). Data are represented as the mean ± standard error of the mean (SEM). *p < 0.05 vs. chow. **p < 0.001 vs. chow. #p < 0.05 vs. TPN. ##p < 0.001 vs. TPN. EN: enteral nutrition; TPN: total parenteral nutrition.
Mentions: Consistent with the results of the protein-content analysis, qPCR analysis of lysozyme revealed thatrelative expression of lysozymewas significantly lower in the TPN groupcompared with the chow group (p = 0.013). In the 10% EN group, expression of lysozyme mRNA was lower than in the chow group (p < 0.05); however, the levels of lysozyme mRNA expression observed in the 20% EN, 40% EN and 60% EN groups were similar to that in chow groups (Figure 5A and Table S3).

Bottom Line: However, the effects of partial EN on intestina linnate immunity, intestinal alkaline phosphatase (IAP) and microbiota remain unclear.Administration of 20% EN supplemented with PN significantly increased the concentrations of lysozyme, MUC2, IAP, and the mRNA levels of lysozyme and MUC2 (p < 0.001).The percentages of Bacteroidetes and Tenericutes were significantly lower in the 20% EN group than in the TPN group (p < 0.001).

View Article: PubMed Central - PubMed

Affiliation: Department of General Surgery, Jinling Hospital, School of Medicine, Nanjing University, Nanjing 210002, China. van395412495@sina.cn.

ABSTRACT
Lack of enteral nutrition (EN) during parenteral nutrition (PN) leads to higher incidence of infection because of gut barrier dysfunction. However, the effects of partial EN on intestina linnate immunity, intestinal alkaline phosphatase (IAP) and microbiota remain unclear. The mice were randomized into six groups to receive either standard chow or isocaloric and isonitrogenous nutritional support with variable partial EN to PN ratios. Five days later, the mice were sacrificed and tissue samples were collected. Bacterial translocation, the levels of lysozyme, mucin 2 (MUC2), and IAP were analyzed. The composition of intestinal microbiota was analyzed by 16S rRNA pyrosequencing. Compared with chow, total parenteral nutrition (TPN) resulted in a dysfunctional mucosal barrier, as evidenced by increased bacterial translocation (p < 0.05), loss of lysozyme, MUC2, and IAP, and changes in the gut microbiota (p < 0.001). Administration of 20% EN supplemented with PN significantly increased the concentrations of lysozyme, MUC2, IAP, and the mRNA levels of lysozyme and MUC2 (p < 0.001). The percentages of Bacteroidetes and Tenericutes were significantly lower in the 20% EN group than in the TPN group (p < 0.001). These changes were accompanied by maintained barrier function in bacterial culture (p < 0.05). Supplementation of PN with 20% EN preserves gut barrier function, by way of maintaining innate immunity, IAP and intestinal microbiota.

No MeSH data available.


Related in: MedlinePlus