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Partial Enteral Nutrition Preserves Elements of Gut Barrier Function, Including Innate Immunity, Intestinal Alkaline Phosphatase (IAP) Level, and Intestinal Microbiota in Mice.

Wan X, Bi J, Gao X, Tian F, Wang X, Li N, Li J - Nutrients (2015)

Bottom Line: However, the effects of partial EN on intestina linnate immunity, intestinal alkaline phosphatase (IAP) and microbiota remain unclear.Administration of 20% EN supplemented with PN significantly increased the concentrations of lysozyme, MUC2, IAP, and the mRNA levels of lysozyme and MUC2 (p < 0.001).The percentages of Bacteroidetes and Tenericutes were significantly lower in the 20% EN group than in the TPN group (p < 0.001).

View Article: PubMed Central - PubMed

Affiliation: Department of General Surgery, Jinling Hospital, School of Medicine, Nanjing University, Nanjing 210002, China. van395412495@sina.cn.

ABSTRACT
Lack of enteral nutrition (EN) during parenteral nutrition (PN) leads to higher incidence of infection because of gut barrier dysfunction. However, the effects of partial EN on intestina linnate immunity, intestinal alkaline phosphatase (IAP) and microbiota remain unclear. The mice were randomized into six groups to receive either standard chow or isocaloric and isonitrogenous nutritional support with variable partial EN to PN ratios. Five days later, the mice were sacrificed and tissue samples were collected. Bacterial translocation, the levels of lysozyme, mucin 2 (MUC2), and IAP were analyzed. The composition of intestinal microbiota was analyzed by 16S rRNA pyrosequencing. Compared with chow, total parenteral nutrition (TPN) resulted in a dysfunctional mucosal barrier, as evidenced by increased bacterial translocation (p < 0.05), loss of lysozyme, MUC2, and IAP, and changes in the gut microbiota (p < 0.001). Administration of 20% EN supplemented with PN significantly increased the concentrations of lysozyme, MUC2, IAP, and the mRNA levels of lysozyme and MUC2 (p < 0.001). The percentages of Bacteroidetes and Tenericutes were significantly lower in the 20% EN group than in the TPN group (p < 0.001). These changes were accompanied by maintained barrier function in bacterial culture (p < 0.05). Supplementation of PN with 20% EN preserves gut barrier function, by way of maintaining innate immunity, IAP and intestinal microbiota.

No MeSH data available.


Related in: MedlinePlus

Lysozyme levels in the ileal tissue. (A) Lysozyme bands, as detected by Western blot analysis; (B) Lysozyme levels in the ileal tissue. Data are presented as the mean ± standard error of the mean (SEM). *p < 0.001 vs. chow. #p < 0.001 vs. TPN; (C) Immunohistochemical analysis of lysozyme in each group. Original magnification: ×40. EN: enteral nutrition; TPN: total parenteral nutrition; GAPDH: Glyceraldehyde-3-phosphate dehydrogenase.
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nutrients-07-05288-f002: Lysozyme levels in the ileal tissue. (A) Lysozyme bands, as detected by Western blot analysis; (B) Lysozyme levels in the ileal tissue. Data are presented as the mean ± standard error of the mean (SEM). *p < 0.001 vs. chow. #p < 0.001 vs. TPN; (C) Immunohistochemical analysis of lysozyme in each group. Original magnification: ×40. EN: enteral nutrition; TPN: total parenteral nutrition; GAPDH: Glyceraldehyde-3-phosphate dehydrogenase.

Mentions: Western blot analysis revealed that lysozyme levels in the ileal tissues of mice from the TPN group were significantly lower than those in the chow group (p < 0.05). The level of tissue lysozyme in the 10% EN group was similar to that in the TPN group; however, the tissue level of lysozyme was four-fold greater in the 20% EN group than in the TPN group (p = 0.01), with a value that was similar to that in the chow group. Lysozyme levels observed in the 40% EN and 60% EN groups were also similar to those in the chow group, but significantly higher than levels in the TPN group (Figure 2A,B and Table S2). The results of IHC analysis for lysozyme were consistent with those of Western blot analysis (Figure 2C).


Partial Enteral Nutrition Preserves Elements of Gut Barrier Function, Including Innate Immunity, Intestinal Alkaline Phosphatase (IAP) Level, and Intestinal Microbiota in Mice.

Wan X, Bi J, Gao X, Tian F, Wang X, Li N, Li J - Nutrients (2015)

Lysozyme levels in the ileal tissue. (A) Lysozyme bands, as detected by Western blot analysis; (B) Lysozyme levels in the ileal tissue. Data are presented as the mean ± standard error of the mean (SEM). *p < 0.001 vs. chow. #p < 0.001 vs. TPN; (C) Immunohistochemical analysis of lysozyme in each group. Original magnification: ×40. EN: enteral nutrition; TPN: total parenteral nutrition; GAPDH: Glyceraldehyde-3-phosphate dehydrogenase.
© Copyright Policy
Related In: Results  -  Collection

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Show All Figures
getmorefigures.php?uid=PMC4555127&req=5

nutrients-07-05288-f002: Lysozyme levels in the ileal tissue. (A) Lysozyme bands, as detected by Western blot analysis; (B) Lysozyme levels in the ileal tissue. Data are presented as the mean ± standard error of the mean (SEM). *p < 0.001 vs. chow. #p < 0.001 vs. TPN; (C) Immunohistochemical analysis of lysozyme in each group. Original magnification: ×40. EN: enteral nutrition; TPN: total parenteral nutrition; GAPDH: Glyceraldehyde-3-phosphate dehydrogenase.
Mentions: Western blot analysis revealed that lysozyme levels in the ileal tissues of mice from the TPN group were significantly lower than those in the chow group (p < 0.05). The level of tissue lysozyme in the 10% EN group was similar to that in the TPN group; however, the tissue level of lysozyme was four-fold greater in the 20% EN group than in the TPN group (p = 0.01), with a value that was similar to that in the chow group. Lysozyme levels observed in the 40% EN and 60% EN groups were also similar to those in the chow group, but significantly higher than levels in the TPN group (Figure 2A,B and Table S2). The results of IHC analysis for lysozyme were consistent with those of Western blot analysis (Figure 2C).

Bottom Line: However, the effects of partial EN on intestina linnate immunity, intestinal alkaline phosphatase (IAP) and microbiota remain unclear.Administration of 20% EN supplemented with PN significantly increased the concentrations of lysozyme, MUC2, IAP, and the mRNA levels of lysozyme and MUC2 (p < 0.001).The percentages of Bacteroidetes and Tenericutes were significantly lower in the 20% EN group than in the TPN group (p < 0.001).

View Article: PubMed Central - PubMed

Affiliation: Department of General Surgery, Jinling Hospital, School of Medicine, Nanjing University, Nanjing 210002, China. van395412495@sina.cn.

ABSTRACT
Lack of enteral nutrition (EN) during parenteral nutrition (PN) leads to higher incidence of infection because of gut barrier dysfunction. However, the effects of partial EN on intestina linnate immunity, intestinal alkaline phosphatase (IAP) and microbiota remain unclear. The mice were randomized into six groups to receive either standard chow or isocaloric and isonitrogenous nutritional support with variable partial EN to PN ratios. Five days later, the mice were sacrificed and tissue samples were collected. Bacterial translocation, the levels of lysozyme, mucin 2 (MUC2), and IAP were analyzed. The composition of intestinal microbiota was analyzed by 16S rRNA pyrosequencing. Compared with chow, total parenteral nutrition (TPN) resulted in a dysfunctional mucosal barrier, as evidenced by increased bacterial translocation (p < 0.05), loss of lysozyme, MUC2, and IAP, and changes in the gut microbiota (p < 0.001). Administration of 20% EN supplemented with PN significantly increased the concentrations of lysozyme, MUC2, IAP, and the mRNA levels of lysozyme and MUC2 (p < 0.001). The percentages of Bacteroidetes and Tenericutes were significantly lower in the 20% EN group than in the TPN group (p < 0.001). These changes were accompanied by maintained barrier function in bacterial culture (p < 0.05). Supplementation of PN with 20% EN preserves gut barrier function, by way of maintaining innate immunity, IAP and intestinal microbiota.

No MeSH data available.


Related in: MedlinePlus