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Partial Enteral Nutrition Preserves Elements of Gut Barrier Function, Including Innate Immunity, Intestinal Alkaline Phosphatase (IAP) Level, and Intestinal Microbiota in Mice.

Wan X, Bi J, Gao X, Tian F, Wang X, Li N, Li J - Nutrients (2015)

Bottom Line: However, the effects of partial EN on intestina linnate immunity, intestinal alkaline phosphatase (IAP) and microbiota remain unclear.Administration of 20% EN supplemented with PN significantly increased the concentrations of lysozyme, MUC2, IAP, and the mRNA levels of lysozyme and MUC2 (p < 0.001).The percentages of Bacteroidetes and Tenericutes were significantly lower in the 20% EN group than in the TPN group (p < 0.001).

View Article: PubMed Central - PubMed

Affiliation: Department of General Surgery, Jinling Hospital, School of Medicine, Nanjing University, Nanjing 210002, China. van395412495@sina.cn.

ABSTRACT
Lack of enteral nutrition (EN) during parenteral nutrition (PN) leads to higher incidence of infection because of gut barrier dysfunction. However, the effects of partial EN on intestina linnate immunity, intestinal alkaline phosphatase (IAP) and microbiota remain unclear. The mice were randomized into six groups to receive either standard chow or isocaloric and isonitrogenous nutritional support with variable partial EN to PN ratios. Five days later, the mice were sacrificed and tissue samples were collected. Bacterial translocation, the levels of lysozyme, mucin 2 (MUC2), and IAP were analyzed. The composition of intestinal microbiota was analyzed by 16S rRNA pyrosequencing. Compared with chow, total parenteral nutrition (TPN) resulted in a dysfunctional mucosal barrier, as evidenced by increased bacterial translocation (p < 0.05), loss of lysozyme, MUC2, and IAP, and changes in the gut microbiota (p < 0.001). Administration of 20% EN supplemented with PN significantly increased the concentrations of lysozyme, MUC2, IAP, and the mRNA levels of lysozyme and MUC2 (p < 0.001). The percentages of Bacteroidetes and Tenericutes were significantly lower in the 20% EN group than in the TPN group (p < 0.001). These changes were accompanied by maintained barrier function in bacterial culture (p < 0.05). Supplementation of PN with 20% EN preserves gut barrier function, by way of maintaining innate immunity, IAP and intestinal microbiota.

No MeSH data available.


Related in: MedlinePlus

Morphological changes in the ileal tissue in each treatment group. (A) Numbers of goblet cells per field of vision; Data are presented as the mean ± standard error of the mean (SEM). **p < 0.001 vs. chow. *p < 0.05 vs. chow. #p < 0.001 vs. TPN; (B) Representative images of periodic acid-Schiff (PAS) base-stained paraffin sections in each group. PAS-stained goblet cells are visible in the epithelial layer (original magnification: ×40). EN: enteral nutrition; TPN: total parenteral nutrition.
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nutrients-07-05288-f001: Morphological changes in the ileal tissue in each treatment group. (A) Numbers of goblet cells per field of vision; Data are presented as the mean ± standard error of the mean (SEM). **p < 0.001 vs. chow. *p < 0.05 vs. chow. #p < 0.001 vs. TPN; (B) Representative images of periodic acid-Schiff (PAS) base-stained paraffin sections in each group. PAS-stained goblet cells are visible in the epithelial layer (original magnification: ×40). EN: enteral nutrition; TPN: total parenteral nutrition.

Mentions: The number of goblet cells per field of vision was significantly lower in the TPN group compared with the chow group (p < 0.001). Supplementation with EN at a dose of 20% resulted in a greater number of goblet cells compared with TPN treatment; however, this value remained lower than the quantity of goblet cells observed in mice from the chow group (p < 0.05). Administration of both 40% and 60% EN led to complete recovery of goblet cell number to a chow level. The average number of cells per group is shown in Figure 1 and Table S1.


Partial Enteral Nutrition Preserves Elements of Gut Barrier Function, Including Innate Immunity, Intestinal Alkaline Phosphatase (IAP) Level, and Intestinal Microbiota in Mice.

Wan X, Bi J, Gao X, Tian F, Wang X, Li N, Li J - Nutrients (2015)

Morphological changes in the ileal tissue in each treatment group. (A) Numbers of goblet cells per field of vision; Data are presented as the mean ± standard error of the mean (SEM). **p < 0.001 vs. chow. *p < 0.05 vs. chow. #p < 0.001 vs. TPN; (B) Representative images of periodic acid-Schiff (PAS) base-stained paraffin sections in each group. PAS-stained goblet cells are visible in the epithelial layer (original magnification: ×40). EN: enteral nutrition; TPN: total parenteral nutrition.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4555127&req=5

nutrients-07-05288-f001: Morphological changes in the ileal tissue in each treatment group. (A) Numbers of goblet cells per field of vision; Data are presented as the mean ± standard error of the mean (SEM). **p < 0.001 vs. chow. *p < 0.05 vs. chow. #p < 0.001 vs. TPN; (B) Representative images of periodic acid-Schiff (PAS) base-stained paraffin sections in each group. PAS-stained goblet cells are visible in the epithelial layer (original magnification: ×40). EN: enteral nutrition; TPN: total parenteral nutrition.
Mentions: The number of goblet cells per field of vision was significantly lower in the TPN group compared with the chow group (p < 0.001). Supplementation with EN at a dose of 20% resulted in a greater number of goblet cells compared with TPN treatment; however, this value remained lower than the quantity of goblet cells observed in mice from the chow group (p < 0.05). Administration of both 40% and 60% EN led to complete recovery of goblet cell number to a chow level. The average number of cells per group is shown in Figure 1 and Table S1.

Bottom Line: However, the effects of partial EN on intestina linnate immunity, intestinal alkaline phosphatase (IAP) and microbiota remain unclear.Administration of 20% EN supplemented with PN significantly increased the concentrations of lysozyme, MUC2, IAP, and the mRNA levels of lysozyme and MUC2 (p < 0.001).The percentages of Bacteroidetes and Tenericutes were significantly lower in the 20% EN group than in the TPN group (p < 0.001).

View Article: PubMed Central - PubMed

Affiliation: Department of General Surgery, Jinling Hospital, School of Medicine, Nanjing University, Nanjing 210002, China. van395412495@sina.cn.

ABSTRACT
Lack of enteral nutrition (EN) during parenteral nutrition (PN) leads to higher incidence of infection because of gut barrier dysfunction. However, the effects of partial EN on intestina linnate immunity, intestinal alkaline phosphatase (IAP) and microbiota remain unclear. The mice were randomized into six groups to receive either standard chow or isocaloric and isonitrogenous nutritional support with variable partial EN to PN ratios. Five days later, the mice were sacrificed and tissue samples were collected. Bacterial translocation, the levels of lysozyme, mucin 2 (MUC2), and IAP were analyzed. The composition of intestinal microbiota was analyzed by 16S rRNA pyrosequencing. Compared with chow, total parenteral nutrition (TPN) resulted in a dysfunctional mucosal barrier, as evidenced by increased bacterial translocation (p < 0.05), loss of lysozyme, MUC2, and IAP, and changes in the gut microbiota (p < 0.001). Administration of 20% EN supplemented with PN significantly increased the concentrations of lysozyme, MUC2, IAP, and the mRNA levels of lysozyme and MUC2 (p < 0.001). The percentages of Bacteroidetes and Tenericutes were significantly lower in the 20% EN group than in the TPN group (p < 0.001). These changes were accompanied by maintained barrier function in bacterial culture (p < 0.05). Supplementation of PN with 20% EN preserves gut barrier function, by way of maintaining innate immunity, IAP and intestinal microbiota.

No MeSH data available.


Related in: MedlinePlus