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Mamao Pomace Extract Alleviates Hypertension and Oxidative Stress in Nitric Oxide Deficient Rats.

Kukongviriyapan U, Kukongviriyapan V, Pannangpetch P, Donpunha W, Sripui J, Sae-Eaw A, Boonla O - Nutrients (2015)

Bottom Line: L-NAME administration induced marked increase in blood pressure, peripheral vascular resistance, and oxidative stress.The antihypertensive effect of MP treatment was associated with suppression of superoxide production from carotid strips and also with an increase in eNOS protein expression and nitric oxide bioavailability.The present results provide evidence for the antihypertensive effect of MP and suggest that MP might be useful as a dietary supplement against hypertension.

View Article: PubMed Central - PubMed

Affiliation: Department of Physiology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand. upa_ku@kku.ac.th.

ABSTRACT
Reactive oxygen species (ROS)-induced oxidative stress plays a major role in pathogenesis of hypertension. Antidesma thwaitesianum (local name: Mamao) is a tropical plant distributed in the tropical/subtropical areas of the world, including Thailand. Mamao pomace (MP), a by-product generated from Mamao fruits, contains large amounts of antioxidant polyphenolic compounds. The aim of this study was to investigate the antihypertensive and antioxidative effects of MP using hypertensive rats. For this purpose, male Sprague-Dawley rats were given Nω-nitro-L-arginine methyl ester (L-NAME), an inhibitor of endothelial nitric oxide synthase (eNOS), in drinking water (50 mg/kg) for three weeks. MP extract was orally administered daily at doses of 100 and 300 mg/kg. L-NAME administration induced marked increase in blood pressure, peripheral vascular resistance, and oxidative stress. MP treatment significantly prevented the increase in blood pressure, hindlimb blood flow and hindlimb vascular resistance of L-NAME treated hypertensive rats (p < 0.05). The antihypertensive effect of MP treatment was associated with suppression of superoxide production from carotid strips and also with an increase in eNOS protein expression and nitric oxide bioavailability. The present results provide evidence for the antihypertensive effect of MP and suggest that MP might be useful as a dietary supplement against hypertension.

No MeSH data available.


Related in: MedlinePlus

Protective effect of mamao pomace extract (MP) on oxidative stress. Plasma malondialdehyde (MDA) (A) and protein carbonyl (B) in normal control and Nω-nitro-l-arginine methyl ester (l-NAME)-treated rats were analyzed. MP was administered at doses of 100 (MP100) and 300 (MP300) mg/kg/day. Each bar represents the mean ± standard error of the mean (SEM) (n = 8–10/group). *p < 0.05 vs. normal control group, #p < 0.05 vs. l-NAME control group, † p < 0.05 vs.l-NAME+ MP100 group.
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nutrients-07-05275-f004: Protective effect of mamao pomace extract (MP) on oxidative stress. Plasma malondialdehyde (MDA) (A) and protein carbonyl (B) in normal control and Nω-nitro-l-arginine methyl ester (l-NAME)-treated rats were analyzed. MP was administered at doses of 100 (MP100) and 300 (MP300) mg/kg/day. Each bar represents the mean ± standard error of the mean (SEM) (n = 8–10/group). *p < 0.05 vs. normal control group, #p < 0.05 vs. l-NAME control group, † p < 0.05 vs.l-NAME+ MP100 group.

Mentions: l-NAME treatment caused oxidative stress in rats, manifested as an increase in O2•− formation, lipid peroxidation and protein oxidation. The l-NAME-treated rats showed a remarkable increase in the release of O2•− from carotid arteries, and this was inhibited by MP treatment (Figure 3). Plasma MDA and plasma protein carbonyl were significantly increased in l-NAME rats and MP treatment prevented these increases (Figure 4A,B).


Mamao Pomace Extract Alleviates Hypertension and Oxidative Stress in Nitric Oxide Deficient Rats.

Kukongviriyapan U, Kukongviriyapan V, Pannangpetch P, Donpunha W, Sripui J, Sae-Eaw A, Boonla O - Nutrients (2015)

Protective effect of mamao pomace extract (MP) on oxidative stress. Plasma malondialdehyde (MDA) (A) and protein carbonyl (B) in normal control and Nω-nitro-l-arginine methyl ester (l-NAME)-treated rats were analyzed. MP was administered at doses of 100 (MP100) and 300 (MP300) mg/kg/day. Each bar represents the mean ± standard error of the mean (SEM) (n = 8–10/group). *p < 0.05 vs. normal control group, #p < 0.05 vs. l-NAME control group, † p < 0.05 vs.l-NAME+ MP100 group.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4555114&req=5

nutrients-07-05275-f004: Protective effect of mamao pomace extract (MP) on oxidative stress. Plasma malondialdehyde (MDA) (A) and protein carbonyl (B) in normal control and Nω-nitro-l-arginine methyl ester (l-NAME)-treated rats were analyzed. MP was administered at doses of 100 (MP100) and 300 (MP300) mg/kg/day. Each bar represents the mean ± standard error of the mean (SEM) (n = 8–10/group). *p < 0.05 vs. normal control group, #p < 0.05 vs. l-NAME control group, † p < 0.05 vs.l-NAME+ MP100 group.
Mentions: l-NAME treatment caused oxidative stress in rats, manifested as an increase in O2•− formation, lipid peroxidation and protein oxidation. The l-NAME-treated rats showed a remarkable increase in the release of O2•− from carotid arteries, and this was inhibited by MP treatment (Figure 3). Plasma MDA and plasma protein carbonyl were significantly increased in l-NAME rats and MP treatment prevented these increases (Figure 4A,B).

Bottom Line: L-NAME administration induced marked increase in blood pressure, peripheral vascular resistance, and oxidative stress.The antihypertensive effect of MP treatment was associated with suppression of superoxide production from carotid strips and also with an increase in eNOS protein expression and nitric oxide bioavailability.The present results provide evidence for the antihypertensive effect of MP and suggest that MP might be useful as a dietary supplement against hypertension.

View Article: PubMed Central - PubMed

Affiliation: Department of Physiology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand. upa_ku@kku.ac.th.

ABSTRACT
Reactive oxygen species (ROS)-induced oxidative stress plays a major role in pathogenesis of hypertension. Antidesma thwaitesianum (local name: Mamao) is a tropical plant distributed in the tropical/subtropical areas of the world, including Thailand. Mamao pomace (MP), a by-product generated from Mamao fruits, contains large amounts of antioxidant polyphenolic compounds. The aim of this study was to investigate the antihypertensive and antioxidative effects of MP using hypertensive rats. For this purpose, male Sprague-Dawley rats were given Nω-nitro-L-arginine methyl ester (L-NAME), an inhibitor of endothelial nitric oxide synthase (eNOS), in drinking water (50 mg/kg) for three weeks. MP extract was orally administered daily at doses of 100 and 300 mg/kg. L-NAME administration induced marked increase in blood pressure, peripheral vascular resistance, and oxidative stress. MP treatment significantly prevented the increase in blood pressure, hindlimb blood flow and hindlimb vascular resistance of L-NAME treated hypertensive rats (p < 0.05). The antihypertensive effect of MP treatment was associated with suppression of superoxide production from carotid strips and also with an increase in eNOS protein expression and nitric oxide bioavailability. The present results provide evidence for the antihypertensive effect of MP and suggest that MP might be useful as a dietary supplement against hypertension.

No MeSH data available.


Related in: MedlinePlus