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Thermosensitive hydrogel containing dexamethasone micelles for preventing postsurgical adhesion in a repeated-injury model.

Wu Q, Wang N, He T, Shang J, Li L, Song L, Yang X, Li X, Luo N, Zhang W, Gong C - Sci Rep (2015)

Bottom Line: The obtained Dex hydrogel showed a temperature-dependent sol-gel-sol phase transition behavior.Compared with normal saline (NS) and Dex micelles group, tissue adhesions in hydrogel and Dex hydrogel group were significantly alleviated.Our results suggested that Dex hydrogel may serve as a potential anti-adhesion candidate.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University, and Collaborative Innovation Center for Biotherapy, Chengdu, 610041, P. R. China.

ABSTRACT
Tissue adhesion is a common complication after surgery. In this work, a dexamethasone loaded polymeric micelles in thermosensitive hydrogel composite (Dex hydrogel) was prepared, which combined the anti-adhesion barrier with controlled release of anti-adhesion drug. Dexamethasone (Dex) was encapsulated in polymeric micelles (Dex micelles), and then the Dex micelles were loaded into biodegradable and thermosensitive hydrogel. The obtained Dex hydrogel showed a temperature-dependent sol-gel-sol phase transition behavior. The Dex hydrogel could form a non-flowing gel in situ upon subcutaneous injection and gradually degrade in about 20 days. In addition, Dex hydrogel was assigned for anti-adhesion studies in a more rigorous recurrent adhesion animal model. Compared with normal saline (NS) and Dex micelles group, tissue adhesions in hydrogel and Dex hydrogel group were significantly alleviated. In Dex hydrogel group, the media adhesion score is 0, which was dramatically lower than that in blank hydrogel group (2.50, P < 0.001). In histopathological examination and scanning electron microscopy (SEM) analysis, an integral neo-mesothelial cell layer with microvilli on their surface was observed, which revealed that the injured parietal and visceral peritoneum were fully recovered without the concerns of adhesion formation. Our results suggested that Dex hydrogel may serve as a potential anti-adhesion candidate.

No MeSH data available.


Related in: MedlinePlus

SEM image of surface of healed abdominal wall (A) and the image with higher magnification (B) in Dex hydrogel group.Microvilli on mesothelial cells were observed.
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f9: SEM image of surface of healed abdominal wall (A) and the image with higher magnification (B) in Dex hydrogel group.Microvilli on mesothelial cells were observed.

Mentions: The recovery of parietal and visceral peritoneal injury and morphology of mesothelial cells were observed using scanning electron microscopy (SEM). Two weeks after the final administration, a layer of elongated, flattened, squamous-like cells located on the surface of recovered parietal peritoneum were observed in Dex hydrogel group (Fig. 9A), and the similar mesothelial cells were also observed in recovered visceral peritoneum (data not shown). Furthermore, in Fig. 9B, a carpet of microvilli on the surface of neo-mesothelial cells in parietal peritoneum occurred in a higher magnification of Fig. 9A. These findings revealed that the injured parietal and visceral peritoneum was fully recovered without the concerns of adhesion formation in the future.


Thermosensitive hydrogel containing dexamethasone micelles for preventing postsurgical adhesion in a repeated-injury model.

Wu Q, Wang N, He T, Shang J, Li L, Song L, Yang X, Li X, Luo N, Zhang W, Gong C - Sci Rep (2015)

SEM image of surface of healed abdominal wall (A) and the image with higher magnification (B) in Dex hydrogel group.Microvilli on mesothelial cells were observed.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4555101&req=5

f9: SEM image of surface of healed abdominal wall (A) and the image with higher magnification (B) in Dex hydrogel group.Microvilli on mesothelial cells were observed.
Mentions: The recovery of parietal and visceral peritoneal injury and morphology of mesothelial cells were observed using scanning electron microscopy (SEM). Two weeks after the final administration, a layer of elongated, flattened, squamous-like cells located on the surface of recovered parietal peritoneum were observed in Dex hydrogel group (Fig. 9A), and the similar mesothelial cells were also observed in recovered visceral peritoneum (data not shown). Furthermore, in Fig. 9B, a carpet of microvilli on the surface of neo-mesothelial cells in parietal peritoneum occurred in a higher magnification of Fig. 9A. These findings revealed that the injured parietal and visceral peritoneum was fully recovered without the concerns of adhesion formation in the future.

Bottom Line: The obtained Dex hydrogel showed a temperature-dependent sol-gel-sol phase transition behavior.Compared with normal saline (NS) and Dex micelles group, tissue adhesions in hydrogel and Dex hydrogel group were significantly alleviated.Our results suggested that Dex hydrogel may serve as a potential anti-adhesion candidate.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University, and Collaborative Innovation Center for Biotherapy, Chengdu, 610041, P. R. China.

ABSTRACT
Tissue adhesion is a common complication after surgery. In this work, a dexamethasone loaded polymeric micelles in thermosensitive hydrogel composite (Dex hydrogel) was prepared, which combined the anti-adhesion barrier with controlled release of anti-adhesion drug. Dexamethasone (Dex) was encapsulated in polymeric micelles (Dex micelles), and then the Dex micelles were loaded into biodegradable and thermosensitive hydrogel. The obtained Dex hydrogel showed a temperature-dependent sol-gel-sol phase transition behavior. The Dex hydrogel could form a non-flowing gel in situ upon subcutaneous injection and gradually degrade in about 20 days. In addition, Dex hydrogel was assigned for anti-adhesion studies in a more rigorous recurrent adhesion animal model. Compared with normal saline (NS) and Dex micelles group, tissue adhesions in hydrogel and Dex hydrogel group were significantly alleviated. In Dex hydrogel group, the media adhesion score is 0, which was dramatically lower than that in blank hydrogel group (2.50, P < 0.001). In histopathological examination and scanning electron microscopy (SEM) analysis, an integral neo-mesothelial cell layer with microvilli on their surface was observed, which revealed that the injured parietal and visceral peritoneum were fully recovered without the concerns of adhesion formation. Our results suggested that Dex hydrogel may serve as a potential anti-adhesion candidate.

No MeSH data available.


Related in: MedlinePlus