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Thermosensitive hydrogel containing dexamethasone micelles for preventing postsurgical adhesion in a repeated-injury model.

Wu Q, Wang N, He T, Shang J, Li L, Song L, Yang X, Li X, Luo N, Zhang W, Gong C - Sci Rep (2015)

Bottom Line: The obtained Dex hydrogel showed a temperature-dependent sol-gel-sol phase transition behavior.Compared with normal saline (NS) and Dex micelles group, tissue adhesions in hydrogel and Dex hydrogel group were significantly alleviated.Our results suggested that Dex hydrogel may serve as a potential anti-adhesion candidate.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University, and Collaborative Innovation Center for Biotherapy, Chengdu, 610041, P. R. China.

ABSTRACT
Tissue adhesion is a common complication after surgery. In this work, a dexamethasone loaded polymeric micelles in thermosensitive hydrogel composite (Dex hydrogel) was prepared, which combined the anti-adhesion barrier with controlled release of anti-adhesion drug. Dexamethasone (Dex) was encapsulated in polymeric micelles (Dex micelles), and then the Dex micelles were loaded into biodegradable and thermosensitive hydrogel. The obtained Dex hydrogel showed a temperature-dependent sol-gel-sol phase transition behavior. The Dex hydrogel could form a non-flowing gel in situ upon subcutaneous injection and gradually degrade in about 20 days. In addition, Dex hydrogel was assigned for anti-adhesion studies in a more rigorous recurrent adhesion animal model. Compared with normal saline (NS) and Dex micelles group, tissue adhesions in hydrogel and Dex hydrogel group were significantly alleviated. In Dex hydrogel group, the media adhesion score is 0, which was dramatically lower than that in blank hydrogel group (2.50, P < 0.001). In histopathological examination and scanning electron microscopy (SEM) analysis, an integral neo-mesothelial cell layer with microvilli on their surface was observed, which revealed that the injured parietal and visceral peritoneum were fully recovered without the concerns of adhesion formation. Our results suggested that Dex hydrogel may serve as a potential anti-adhesion candidate.

No MeSH data available.


Related in: MedlinePlus

H&E and Masson’s trichrome staining of healed abdominal wall (A,B) and cecum (C,D) in Dex hydrogel group.
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f8: H&E and Masson’s trichrome staining of healed abdominal wall (A,B) and cecum (C,D) in Dex hydrogel group.

Mentions: On histological examination, tissue samples from injured abdominal wall and cecum were stained with hematoxylin-eosin (H&E) and Masson trichrome staining (Fig. 7). As shown in Fig. 7, close apposition of the abdominal wall musculature and the smooth muscle layers of cecum were connected by various fibrous tissues in the tissues from adhesion sits in NS group, and the same condition was also observed in Dex micelles group (data not shown). In contrast, an integral neo-mesothelial cell layer with various subjacent fibrosis was observed in both abdominal wall and cecum in Dex hydrogel group (Fig. 8), which meant the injured abdominal wall and cecum were fully recovered without the concern of adhesion.


Thermosensitive hydrogel containing dexamethasone micelles for preventing postsurgical adhesion in a repeated-injury model.

Wu Q, Wang N, He T, Shang J, Li L, Song L, Yang X, Li X, Luo N, Zhang W, Gong C - Sci Rep (2015)

H&E and Masson’s trichrome staining of healed abdominal wall (A,B) and cecum (C,D) in Dex hydrogel group.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4555101&req=5

f8: H&E and Masson’s trichrome staining of healed abdominal wall (A,B) and cecum (C,D) in Dex hydrogel group.
Mentions: On histological examination, tissue samples from injured abdominal wall and cecum were stained with hematoxylin-eosin (H&E) and Masson trichrome staining (Fig. 7). As shown in Fig. 7, close apposition of the abdominal wall musculature and the smooth muscle layers of cecum were connected by various fibrous tissues in the tissues from adhesion sits in NS group, and the same condition was also observed in Dex micelles group (data not shown). In contrast, an integral neo-mesothelial cell layer with various subjacent fibrosis was observed in both abdominal wall and cecum in Dex hydrogel group (Fig. 8), which meant the injured abdominal wall and cecum were fully recovered without the concern of adhesion.

Bottom Line: The obtained Dex hydrogel showed a temperature-dependent sol-gel-sol phase transition behavior.Compared with normal saline (NS) and Dex micelles group, tissue adhesions in hydrogel and Dex hydrogel group were significantly alleviated.Our results suggested that Dex hydrogel may serve as a potential anti-adhesion candidate.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University, and Collaborative Innovation Center for Biotherapy, Chengdu, 610041, P. R. China.

ABSTRACT
Tissue adhesion is a common complication after surgery. In this work, a dexamethasone loaded polymeric micelles in thermosensitive hydrogel composite (Dex hydrogel) was prepared, which combined the anti-adhesion barrier with controlled release of anti-adhesion drug. Dexamethasone (Dex) was encapsulated in polymeric micelles (Dex micelles), and then the Dex micelles were loaded into biodegradable and thermosensitive hydrogel. The obtained Dex hydrogel showed a temperature-dependent sol-gel-sol phase transition behavior. The Dex hydrogel could form a non-flowing gel in situ upon subcutaneous injection and gradually degrade in about 20 days. In addition, Dex hydrogel was assigned for anti-adhesion studies in a more rigorous recurrent adhesion animal model. Compared with normal saline (NS) and Dex micelles group, tissue adhesions in hydrogel and Dex hydrogel group were significantly alleviated. In Dex hydrogel group, the media adhesion score is 0, which was dramatically lower than that in blank hydrogel group (2.50, P < 0.001). In histopathological examination and scanning electron microscopy (SEM) analysis, an integral neo-mesothelial cell layer with microvilli on their surface was observed, which revealed that the injured parietal and visceral peritoneum were fully recovered without the concerns of adhesion formation. Our results suggested that Dex hydrogel may serve as a potential anti-adhesion candidate.

No MeSH data available.


Related in: MedlinePlus