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Thermosensitive hydrogel containing dexamethasone micelles for preventing postsurgical adhesion in a repeated-injury model.

Wu Q, Wang N, He T, Shang J, Li L, Song L, Yang X, Li X, Luo N, Zhang W, Gong C - Sci Rep (2015)

Bottom Line: The obtained Dex hydrogel showed a temperature-dependent sol-gel-sol phase transition behavior.Compared with normal saline (NS) and Dex micelles group, tissue adhesions in hydrogel and Dex hydrogel group were significantly alleviated.Our results suggested that Dex hydrogel may serve as a potential anti-adhesion candidate.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University, and Collaborative Innovation Center for Biotherapy, Chengdu, 610041, P. R. China.

ABSTRACT
Tissue adhesion is a common complication after surgery. In this work, a dexamethasone loaded polymeric micelles in thermosensitive hydrogel composite (Dex hydrogel) was prepared, which combined the anti-adhesion barrier with controlled release of anti-adhesion drug. Dexamethasone (Dex) was encapsulated in polymeric micelles (Dex micelles), and then the Dex micelles were loaded into biodegradable and thermosensitive hydrogel. The obtained Dex hydrogel showed a temperature-dependent sol-gel-sol phase transition behavior. The Dex hydrogel could form a non-flowing gel in situ upon subcutaneous injection and gradually degrade in about 20 days. In addition, Dex hydrogel was assigned for anti-adhesion studies in a more rigorous recurrent adhesion animal model. Compared with normal saline (NS) and Dex micelles group, tissue adhesions in hydrogel and Dex hydrogel group were significantly alleviated. In Dex hydrogel group, the media adhesion score is 0, which was dramatically lower than that in blank hydrogel group (2.50, P < 0.001). In histopathological examination and scanning electron microscopy (SEM) analysis, an integral neo-mesothelial cell layer with microvilli on their surface was observed, which revealed that the injured parietal and visceral peritoneum were fully recovered without the concerns of adhesion formation. Our results suggested that Dex hydrogel may serve as a potential anti-adhesion candidate.

No MeSH data available.


Related in: MedlinePlus

Evaluation of adhesion prevention in NS (A), Dex micelles (B), hydrogel (C), and Dex hydrogel (D) group.
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f6: Evaluation of adhesion prevention in NS (A), Dex micelles (B), hydrogel (C), and Dex hydrogel (D) group.

Mentions: Rat abdominal wall defect-cecum abrasion peritoneal adhesion model was established (Fig. 5A). One week later, a second laparotomy was performed, in which adhesions were cut by a blunt or sharp dissection as needed. The separated abdominal wall and cecal surface were re-abraded with a sterile brush until a bleeding surface was produced (Fig. 5B). Before complete closure, 1 mL of Dex hydrogel, Dex micelles, blank hydrogel or normal saline (NS) was applied to coat the both damaged surfaces and the un-damaged surfaces around (Fig. 5C). Then, two weeks later, the rats were sacrified to examine the anti-adhesion effects (Fig. 6 and Table 1). In NS group, 7 of 8 rats developed score 5 score adhesion, and the other one developed score 4 adhesion, which indicated that the repeated-injury adhesion model was established successfully (Fig. 6A). Dex micelles group only showed a very slight improvement in adhesion prevention, which may due to the fast absorption and metabolism of Dex micelles in abdominal cavity (Fig. 6B). In addition, compared with NS and Dex micelles group, tissue adhesions in hydrogel and Dex hydrogel group were significantly alleviated (Fig. 6C,D). In Dex hydrogel group, the media score is 0, which was dramatically lower than that in blank hydrogel group (2.50, P < 0.01, Mann-Whitney U-tests).


Thermosensitive hydrogel containing dexamethasone micelles for preventing postsurgical adhesion in a repeated-injury model.

Wu Q, Wang N, He T, Shang J, Li L, Song L, Yang X, Li X, Luo N, Zhang W, Gong C - Sci Rep (2015)

Evaluation of adhesion prevention in NS (A), Dex micelles (B), hydrogel (C), and Dex hydrogel (D) group.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4555101&req=5

f6: Evaluation of adhesion prevention in NS (A), Dex micelles (B), hydrogel (C), and Dex hydrogel (D) group.
Mentions: Rat abdominal wall defect-cecum abrasion peritoneal adhesion model was established (Fig. 5A). One week later, a second laparotomy was performed, in which adhesions were cut by a blunt or sharp dissection as needed. The separated abdominal wall and cecal surface were re-abraded with a sterile brush until a bleeding surface was produced (Fig. 5B). Before complete closure, 1 mL of Dex hydrogel, Dex micelles, blank hydrogel or normal saline (NS) was applied to coat the both damaged surfaces and the un-damaged surfaces around (Fig. 5C). Then, two weeks later, the rats were sacrified to examine the anti-adhesion effects (Fig. 6 and Table 1). In NS group, 7 of 8 rats developed score 5 score adhesion, and the other one developed score 4 adhesion, which indicated that the repeated-injury adhesion model was established successfully (Fig. 6A). Dex micelles group only showed a very slight improvement in adhesion prevention, which may due to the fast absorption and metabolism of Dex micelles in abdominal cavity (Fig. 6B). In addition, compared with NS and Dex micelles group, tissue adhesions in hydrogel and Dex hydrogel group were significantly alleviated (Fig. 6C,D). In Dex hydrogel group, the media score is 0, which was dramatically lower than that in blank hydrogel group (2.50, P < 0.01, Mann-Whitney U-tests).

Bottom Line: The obtained Dex hydrogel showed a temperature-dependent sol-gel-sol phase transition behavior.Compared with normal saline (NS) and Dex micelles group, tissue adhesions in hydrogel and Dex hydrogel group were significantly alleviated.Our results suggested that Dex hydrogel may serve as a potential anti-adhesion candidate.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University, and Collaborative Innovation Center for Biotherapy, Chengdu, 610041, P. R. China.

ABSTRACT
Tissue adhesion is a common complication after surgery. In this work, a dexamethasone loaded polymeric micelles in thermosensitive hydrogel composite (Dex hydrogel) was prepared, which combined the anti-adhesion barrier with controlled release of anti-adhesion drug. Dexamethasone (Dex) was encapsulated in polymeric micelles (Dex micelles), and then the Dex micelles were loaded into biodegradable and thermosensitive hydrogel. The obtained Dex hydrogel showed a temperature-dependent sol-gel-sol phase transition behavior. The Dex hydrogel could form a non-flowing gel in situ upon subcutaneous injection and gradually degrade in about 20 days. In addition, Dex hydrogel was assigned for anti-adhesion studies in a more rigorous recurrent adhesion animal model. Compared with normal saline (NS) and Dex micelles group, tissue adhesions in hydrogel and Dex hydrogel group were significantly alleviated. In Dex hydrogel group, the media adhesion score is 0, which was dramatically lower than that in blank hydrogel group (2.50, P < 0.001). In histopathological examination and scanning electron microscopy (SEM) analysis, an integral neo-mesothelial cell layer with microvilli on their surface was observed, which revealed that the injured parietal and visceral peritoneum were fully recovered without the concerns of adhesion formation. Our results suggested that Dex hydrogel may serve as a potential anti-adhesion candidate.

No MeSH data available.


Related in: MedlinePlus