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Inhibitory effects of magnolol and honokiol on human calcitonin aggregation.

Guo C, Ma L, Zhao Y, Peng A, Cheng B, Zhou Q, Zheng L, Huang K - Sci Rep (2015)

Bottom Line: In this study, these two compounds were tested for their effects on hCT aggregation.Further immuno-dot blot and dynamic light scattering studies suggested Mag and Hon suppressed the aggregation of hCT both at the oligomerization and the fibrillation stages, while MTT-based and dye-leakage assays demonstrated that Mag and Hon effectively reduced cytotoxicity caused by hCT aggregates.Together, our study suggested a potential anti-amyloidogenic property of these two compounds and their structure related derivatives.

View Article: PubMed Central - PubMed

Affiliation: Tongji School of Pharmacy, Huazhong University of Science and Technology, Wuhan, Hubei, P. R. China, 430030.

ABSTRACT
Amyloid formation is associated with multiple amyloidosis diseases. Human calcitonin (hCT) is a typical amyloidogenic peptide, its aggregation is associated with medullary carcinoma of the thyroid (MTC), and also limits its clinical application. Magnolia officinalis is a traditional Chinese herbal medicine; its two major polyphenol components, magnolol (Mag) and honokiol (Hon), have displayed multiple functions. Polyphenols like flavonoids and their derivatives have been extensively studied as amyloid inhibitors. However, the anti-amyloidogenic property of a biphenyl backbone containing polyphenols such as Mag and Hon has not been reported. In this study, these two compounds were tested for their effects on hCT aggregation. We found that Mag and Hon both inhibited the amyloid formation of hCT, whereas Mag showed a stronger inhibitory effect; moreover, they both dose-dependently disassembled preformed hCT aggregates. Further immuno-dot blot and dynamic light scattering studies suggested Mag and Hon suppressed the aggregation of hCT both at the oligomerization and the fibrillation stages, while MTT-based and dye-leakage assays demonstrated that Mag and Hon effectively reduced cytotoxicity caused by hCT aggregates. Furthermore, isothermal titration calorimetry indicated Mag and Hon both interact with hCT. Together, our study suggested a potential anti-amyloidogenic property of these two compounds and their structure related derivatives.

No MeSH data available.


Related in: MedlinePlus

Inhibitory effects of magnolol and honokiol on hCT oligomerization and fibrillation detected by dot blot.
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f3: Inhibitory effects of magnolol and honokiol on hCT oligomerization and fibrillation detected by dot blot.

Mentions: The effects of Mag and Hon on hCT oligomerization and fibrillation were further determined by immuno-dot blot. A11 and OC antibodies were applied to detect the formation of oligomers and fibrils, respectively5758. For hCT, A11-positive oligomers were observed after 24 h of incubation, while OC-positive fibrils emerged after 36 h incubation (Fig. 3). In the presence of equimolar amount of EGCG, neither A11-positive oligomers nor OC-positive fibrils were observed after 72 h of incubation, which agrees with the TEM results (Fig. 2D).


Inhibitory effects of magnolol and honokiol on human calcitonin aggregation.

Guo C, Ma L, Zhao Y, Peng A, Cheng B, Zhou Q, Zheng L, Huang K - Sci Rep (2015)

Inhibitory effects of magnolol and honokiol on hCT oligomerization and fibrillation detected by dot blot.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4555095&req=5

f3: Inhibitory effects of magnolol and honokiol on hCT oligomerization and fibrillation detected by dot blot.
Mentions: The effects of Mag and Hon on hCT oligomerization and fibrillation were further determined by immuno-dot blot. A11 and OC antibodies were applied to detect the formation of oligomers and fibrils, respectively5758. For hCT, A11-positive oligomers were observed after 24 h of incubation, while OC-positive fibrils emerged after 36 h incubation (Fig. 3). In the presence of equimolar amount of EGCG, neither A11-positive oligomers nor OC-positive fibrils were observed after 72 h of incubation, which agrees with the TEM results (Fig. 2D).

Bottom Line: In this study, these two compounds were tested for their effects on hCT aggregation.Further immuno-dot blot and dynamic light scattering studies suggested Mag and Hon suppressed the aggregation of hCT both at the oligomerization and the fibrillation stages, while MTT-based and dye-leakage assays demonstrated that Mag and Hon effectively reduced cytotoxicity caused by hCT aggregates.Together, our study suggested a potential anti-amyloidogenic property of these two compounds and their structure related derivatives.

View Article: PubMed Central - PubMed

Affiliation: Tongji School of Pharmacy, Huazhong University of Science and Technology, Wuhan, Hubei, P. R. China, 430030.

ABSTRACT
Amyloid formation is associated with multiple amyloidosis diseases. Human calcitonin (hCT) is a typical amyloidogenic peptide, its aggregation is associated with medullary carcinoma of the thyroid (MTC), and also limits its clinical application. Magnolia officinalis is a traditional Chinese herbal medicine; its two major polyphenol components, magnolol (Mag) and honokiol (Hon), have displayed multiple functions. Polyphenols like flavonoids and their derivatives have been extensively studied as amyloid inhibitors. However, the anti-amyloidogenic property of a biphenyl backbone containing polyphenols such as Mag and Hon has not been reported. In this study, these two compounds were tested for their effects on hCT aggregation. We found that Mag and Hon both inhibited the amyloid formation of hCT, whereas Mag showed a stronger inhibitory effect; moreover, they both dose-dependently disassembled preformed hCT aggregates. Further immuno-dot blot and dynamic light scattering studies suggested Mag and Hon suppressed the aggregation of hCT both at the oligomerization and the fibrillation stages, while MTT-based and dye-leakage assays demonstrated that Mag and Hon effectively reduced cytotoxicity caused by hCT aggregates. Furthermore, isothermal titration calorimetry indicated Mag and Hon both interact with hCT. Together, our study suggested a potential anti-amyloidogenic property of these two compounds and their structure related derivatives.

No MeSH data available.


Related in: MedlinePlus