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Identification of Recurrence-Related microRNAs from Bone Marrow in Hepatocellular Carcinoma Patients.

Sugimachi K, Sakimura S, Tomokuni A, Uchi R, Hirata H, Komatsu H, Shinden Y, Iguchi T, Eguchi H, Masuda T, Morita K, Shirabe K, Eguchi H, Maehara Y, Mori M, Mimori K - J Clin Med (2015)

Bottom Line: Differentially expressed miRNAs were significantly different between fractions from whole bone marrow, macrophages, and lymphocytes, and depended on stages in tumor progression.Differences in expression of miRNAs associated with cell proliferation also varied significantly between HCC patients with recurrence, multiple tumors, and advanced clinical stages.These results suggest that miRNA profiles in separated fractions of BM cells are associated with HCC progression.

View Article: PubMed Central - PubMed

Affiliation: Department of Surgery, Kyushu University Beppu Hospital, 4546 Tsurumihara, Beppu 874-0838, Japan. kamorita@surg2.med.kyushu-u.ac.jp.

ABSTRACT
Hepatocellular carcinoma (HCC) is a poor-prognosis cancer due to its high rate of recurrence. microRNAs (miRNAs) are a class of small non-coding RNA molecules that affect crucial processes in cancer development. The objective of this study is to identify the role of miRNAs in patient bone marrow (BM) and explore the function of these molecules during HCC progression. We purified miRNAs from bone marrow cells of seven HCC patients, and divided them into three fractions by cell surface markers as follows: CD14(+) (macrophage), CD14(-)/CD45(+) (lymphocyte), and CD14(-)/CD45(-)/EpCAM(+) (epithelial cell). We employed microarray-based profiling to analyze miRNA expression in the bone marrow of patients with HCC. Differentially expressed miRNAs were significantly different between fractions from whole bone marrow, macrophages, and lymphocytes, and depended on stages in tumor progression. Differences in expression of miRNAs associated with cell proliferation also varied significantly between HCC patients with recurrence, multiple tumors, and advanced clinical stages. These results suggest that miRNA profiles in separated fractions of BM cells are associated with HCC progression.

No MeSH data available.


Related in: MedlinePlus

Hierarchical clustering analysis of microRNAs of macrophage fraction in seven patients with hepatocellular carcinoma. Heat map of the miRNA profile in lymphocytes from hepatocellular carcinoma patients with (a) recurrence (n = 3) and no recurrence (n = 4); (b) solitary (n = 5) and multiple tumors (n = 2); (c) stage 1 (n = 3) and ≥stage 2 (n = 4). Cluster analysis showed eight, seven, and six miRNAs that were significantly differentially expressed between the two groups with a >2-fold change, respectively. Colors range from blue to red, corresponding to low to high expression, respectively. p values <0.01 or <0.05, unpaired t test. rec: recurrence.
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jcm-04-01600-f003: Hierarchical clustering analysis of microRNAs of macrophage fraction in seven patients with hepatocellular carcinoma. Heat map of the miRNA profile in lymphocytes from hepatocellular carcinoma patients with (a) recurrence (n = 3) and no recurrence (n = 4); (b) solitary (n = 5) and multiple tumors (n = 2); (c) stage 1 (n = 3) and ≥stage 2 (n = 4). Cluster analysis showed eight, seven, and six miRNAs that were significantly differentially expressed between the two groups with a >2-fold change, respectively. Colors range from blue to red, corresponding to low to high expression, respectively. p values <0.01 or <0.05, unpaired t test. rec: recurrence.

Mentions: Two miRNAs were upregulated (hsa-miR-1207-3p, 937) and six miRNAs (hsa-miR-1277, 1279, 184, 563, 96, 302b) were downregulated in the macrophage fractions from cases with post-operative recurrence compared to fractions from cases with no recurrence (Figure 3 and Table 1).


Identification of Recurrence-Related microRNAs from Bone Marrow in Hepatocellular Carcinoma Patients.

Sugimachi K, Sakimura S, Tomokuni A, Uchi R, Hirata H, Komatsu H, Shinden Y, Iguchi T, Eguchi H, Masuda T, Morita K, Shirabe K, Eguchi H, Maehara Y, Mori M, Mimori K - J Clin Med (2015)

Hierarchical clustering analysis of microRNAs of macrophage fraction in seven patients with hepatocellular carcinoma. Heat map of the miRNA profile in lymphocytes from hepatocellular carcinoma patients with (a) recurrence (n = 3) and no recurrence (n = 4); (b) solitary (n = 5) and multiple tumors (n = 2); (c) stage 1 (n = 3) and ≥stage 2 (n = 4). Cluster analysis showed eight, seven, and six miRNAs that were significantly differentially expressed between the two groups with a >2-fold change, respectively. Colors range from blue to red, corresponding to low to high expression, respectively. p values <0.01 or <0.05, unpaired t test. rec: recurrence.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4555079&req=5

jcm-04-01600-f003: Hierarchical clustering analysis of microRNAs of macrophage fraction in seven patients with hepatocellular carcinoma. Heat map of the miRNA profile in lymphocytes from hepatocellular carcinoma patients with (a) recurrence (n = 3) and no recurrence (n = 4); (b) solitary (n = 5) and multiple tumors (n = 2); (c) stage 1 (n = 3) and ≥stage 2 (n = 4). Cluster analysis showed eight, seven, and six miRNAs that were significantly differentially expressed between the two groups with a >2-fold change, respectively. Colors range from blue to red, corresponding to low to high expression, respectively. p values <0.01 or <0.05, unpaired t test. rec: recurrence.
Mentions: Two miRNAs were upregulated (hsa-miR-1207-3p, 937) and six miRNAs (hsa-miR-1277, 1279, 184, 563, 96, 302b) were downregulated in the macrophage fractions from cases with post-operative recurrence compared to fractions from cases with no recurrence (Figure 3 and Table 1).

Bottom Line: Differentially expressed miRNAs were significantly different between fractions from whole bone marrow, macrophages, and lymphocytes, and depended on stages in tumor progression.Differences in expression of miRNAs associated with cell proliferation also varied significantly between HCC patients with recurrence, multiple tumors, and advanced clinical stages.These results suggest that miRNA profiles in separated fractions of BM cells are associated with HCC progression.

View Article: PubMed Central - PubMed

Affiliation: Department of Surgery, Kyushu University Beppu Hospital, 4546 Tsurumihara, Beppu 874-0838, Japan. kamorita@surg2.med.kyushu-u.ac.jp.

ABSTRACT
Hepatocellular carcinoma (HCC) is a poor-prognosis cancer due to its high rate of recurrence. microRNAs (miRNAs) are a class of small non-coding RNA molecules that affect crucial processes in cancer development. The objective of this study is to identify the role of miRNAs in patient bone marrow (BM) and explore the function of these molecules during HCC progression. We purified miRNAs from bone marrow cells of seven HCC patients, and divided them into three fractions by cell surface markers as follows: CD14(+) (macrophage), CD14(-)/CD45(+) (lymphocyte), and CD14(-)/CD45(-)/EpCAM(+) (epithelial cell). We employed microarray-based profiling to analyze miRNA expression in the bone marrow of patients with HCC. Differentially expressed miRNAs were significantly different between fractions from whole bone marrow, macrophages, and lymphocytes, and depended on stages in tumor progression. Differences in expression of miRNAs associated with cell proliferation also varied significantly between HCC patients with recurrence, multiple tumors, and advanced clinical stages. These results suggest that miRNA profiles in separated fractions of BM cells are associated with HCC progression.

No MeSH data available.


Related in: MedlinePlus