Limits...
Effects of human menopausal gonadotropin on zona pellucida and pregnancy outcomes of ovarian stimulation protocols.

He B, Junping C, Li H, Weihong T, Lintao X, Shikai W - Iran J Reprod Med (2015)

Bottom Line: However, effects of hMG on oocyte development and clinical results remain controversial.No significant differences were existed in r-FSH +hMG, and r-FSH groups in the number of retrieved oocytes (11.99±0.75 vs. 13.9±0.73, p=0.06), maturation rate (84.76% vs. 83.32%, p=0.42), pregnancy rate (37.31% vs. 37.66%, p=0.96), and embryo implantation rate (28.97% vs. 23.26%, p=0.30).However, fertilization rate (82.95% vs. 78.75%; p=0.02) was different.

View Article: PubMed Central - PubMed

Affiliation: People's Hospital of Guangxi Autonomous Region, Nanning, 530021, China.

ABSTRACT

Background: Human menopausal gonadotropin (hMG) has contributed many improvements to human assisted reproduction. However, effects of hMG on oocyte development and clinical results remain controversial.

Objective: This study was conducted to investigate the effects of hMG on the zona pellucida of oocytes, as well as clinical results in superovulation treatment.

Materials and methods: This retrospective study was performed with 150 cycles of long-protocol treatment using recombinant follicle-stimulating hormone (r-FSH) with or without hMG. The number of retrieved oocytes, fertilization rate, implantation rate, pregnancy rate, and birefringence and thickness of the zona pellucida of oocytes were investigated.

Results: No significant differences were existed in r-FSH +hMG, and r-FSH groups in the number of retrieved oocytes (11.99±0.75 vs. 13.9±0.73, p=0.06), maturation rate (84.76% vs. 83.32%, p=0.42), pregnancy rate (37.31% vs. 37.66%, p=0.96), and embryo implantation rate (28.97% vs. 23.26%, p=0.30). However, fertilization rate (82.95% vs. 78.75%; p=0.02) was different. Zona pellucida birefringence was lower in the r-FSH +hMG group than in the r-FSH group (6.70±0.50 vs. 7.04±0.31; p=0.53). Thickness values of the metaphase-II zona pellucida of the r-FSH +hMG group on the first (19.20±0.14 vs. 18.75±0.10; p=0.01) and second (18.69±0.12 vs. 18.17±0.14; p=0.00) days of insemination were both higher than those of the r-FSH group.

Conclusion: hMG positively influenced the improvement of oocyte fertilization, as well as the birefringence and thickness of zona pellucida.

No MeSH data available.


Related in: MedlinePlus

Examination of the zona pellucida birefringence scores of MII oocytes
© Copyright Policy
Related In: Results  -  Collection


getmorefigures.php?uid=PMC4555052&req=5

Figure 1: Examination of the zona pellucida birefringence scores of MII oocytes

Mentions: ZPB (Figure 1) and ZPT (Figure 2) were detected using a previously described method (15). Denude oocytes were transferred separately into 3-5 µL pre-warmed droplets of GB medium (COOK) overlaid with equilibrated mineral oil (Sage, Trumbull, CT, USA) in glass-bottomed dishes. The ZP was imaged separately using an Olympus IX71 microscope with the OCTAX Polar-AIDE system (Octax) with an oocyte equator, in which the oolemmas were clear. Data were stored for later analysis but not used for embryo selection.


Effects of human menopausal gonadotropin on zona pellucida and pregnancy outcomes of ovarian stimulation protocols.

He B, Junping C, Li H, Weihong T, Lintao X, Shikai W - Iran J Reprod Med (2015)

Examination of the zona pellucida birefringence scores of MII oocytes
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4555052&req=5

Figure 1: Examination of the zona pellucida birefringence scores of MII oocytes
Mentions: ZPB (Figure 1) and ZPT (Figure 2) were detected using a previously described method (15). Denude oocytes were transferred separately into 3-5 µL pre-warmed droplets of GB medium (COOK) overlaid with equilibrated mineral oil (Sage, Trumbull, CT, USA) in glass-bottomed dishes. The ZP was imaged separately using an Olympus IX71 microscope with the OCTAX Polar-AIDE system (Octax) with an oocyte equator, in which the oolemmas were clear. Data were stored for later analysis but not used for embryo selection.

Bottom Line: However, effects of hMG on oocyte development and clinical results remain controversial.No significant differences were existed in r-FSH +hMG, and r-FSH groups in the number of retrieved oocytes (11.99±0.75 vs. 13.9±0.73, p=0.06), maturation rate (84.76% vs. 83.32%, p=0.42), pregnancy rate (37.31% vs. 37.66%, p=0.96), and embryo implantation rate (28.97% vs. 23.26%, p=0.30).However, fertilization rate (82.95% vs. 78.75%; p=0.02) was different.

View Article: PubMed Central - PubMed

Affiliation: People's Hospital of Guangxi Autonomous Region, Nanning, 530021, China.

ABSTRACT

Background: Human menopausal gonadotropin (hMG) has contributed many improvements to human assisted reproduction. However, effects of hMG on oocyte development and clinical results remain controversial.

Objective: This study was conducted to investigate the effects of hMG on the zona pellucida of oocytes, as well as clinical results in superovulation treatment.

Materials and methods: This retrospective study was performed with 150 cycles of long-protocol treatment using recombinant follicle-stimulating hormone (r-FSH) with or without hMG. The number of retrieved oocytes, fertilization rate, implantation rate, pregnancy rate, and birefringence and thickness of the zona pellucida of oocytes were investigated.

Results: No significant differences were existed in r-FSH +hMG, and r-FSH groups in the number of retrieved oocytes (11.99±0.75 vs. 13.9±0.73, p=0.06), maturation rate (84.76% vs. 83.32%, p=0.42), pregnancy rate (37.31% vs. 37.66%, p=0.96), and embryo implantation rate (28.97% vs. 23.26%, p=0.30). However, fertilization rate (82.95% vs. 78.75%; p=0.02) was different. Zona pellucida birefringence was lower in the r-FSH +hMG group than in the r-FSH group (6.70±0.50 vs. 7.04±0.31; p=0.53). Thickness values of the metaphase-II zona pellucida of the r-FSH +hMG group on the first (19.20±0.14 vs. 18.75±0.10; p=0.01) and second (18.69±0.12 vs. 18.17±0.14; p=0.00) days of insemination were both higher than those of the r-FSH group.

Conclusion: hMG positively influenced the improvement of oocyte fertilization, as well as the birefringence and thickness of zona pellucida.

No MeSH data available.


Related in: MedlinePlus