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Transcriptional modulation of squalene synthase genes in barley treated with PGPR.

Yousaf A, Qadir A, Anjum T, Ahmad A - Front Plant Sci (2015)

Bottom Line: Phytosterol contents and food quality of plant produce is directly associated with transcription of gene squalene synthase (SS).Plant samples were analyzed through semi-quantitative PCR to evaluate effect of rhizobacterial application on transcriptional status of SS.The study would be helpful to improve quality of plant produce by modulating transcription of SS genes.

View Article: PubMed Central - PubMed

Affiliation: College of Earth and Environmental Sciences, University of the Punjab, Lahore Pakistan.

ABSTRACT
Phytosterol contents and food quality of plant produce is directly associated with transcription of gene squalene synthase (SS). In current study, barley plants were treated with different rhizobacterial strains under semi controlled (27 ± 3°C) greenhouse conditions in order to modulate expression of SS gene. Plant samples were analyzed through semi-quantitative PCR to evaluate effect of rhizobacterial application on transcriptional status of SS. Results revealed that among four SS genes (i.e., SSA, SS1, SS2, and SS3), the most expressive gene was SSA; while, SS2 was screened out as the second best induced gene due to Acetobacter aceti. The most efficient bacterial strain which recorded maximum gene expression was A. aceti AC8. Moreover, AC7 was reported as the least efficient bacterial species for inducing SS gene expression. AC8 enhanced the share of SSA and SS2 up to 43 and 31%, respectively. The study also described ribosomal sequence of the most efficient bacterial strain AC8, which was used to determine its phylogenetic relationships with other microbial strains. The study would be helpful to improve quality of plant produce by modulating transcription of SS genes.

No MeSH data available.


Related in: MedlinePlus

PCR amplification of 16S sequence of Acetobacter aceti(A), and its dendrogram in comparison to other reported sequences of the same bacterial species based upon similarity index (B). Values mentioned against each strain show the phylogenetic distances of different bacterial strains.
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Figure 6: PCR amplification of 16S sequence of Acetobacter aceti(A), and its dendrogram in comparison to other reported sequences of the same bacterial species based upon similarity index (B). Values mentioned against each strain show the phylogenetic distances of different bacterial strains.

Mentions: Ribotyping of bacterial species generated a sequence of 600 bases which was submitted to GenBank under the accession number # KR024029 (Figure 6A). BLAST analysis of the sequence showed ≥97% similarity with previously reported sequences of A. aceti (Figure 6B). The sequence recorded the maximum similarity with strains ZIM B043, LMG-28092, and LMG-27543 of A. aceti (Accession # AJ012542.1, KJ147512.1, and HG424426.1, respectively).


Transcriptional modulation of squalene synthase genes in barley treated with PGPR.

Yousaf A, Qadir A, Anjum T, Ahmad A - Front Plant Sci (2015)

PCR amplification of 16S sequence of Acetobacter aceti(A), and its dendrogram in comparison to other reported sequences of the same bacterial species based upon similarity index (B). Values mentioned against each strain show the phylogenetic distances of different bacterial strains.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4555044&req=5

Figure 6: PCR amplification of 16S sequence of Acetobacter aceti(A), and its dendrogram in comparison to other reported sequences of the same bacterial species based upon similarity index (B). Values mentioned against each strain show the phylogenetic distances of different bacterial strains.
Mentions: Ribotyping of bacterial species generated a sequence of 600 bases which was submitted to GenBank under the accession number # KR024029 (Figure 6A). BLAST analysis of the sequence showed ≥97% similarity with previously reported sequences of A. aceti (Figure 6B). The sequence recorded the maximum similarity with strains ZIM B043, LMG-28092, and LMG-27543 of A. aceti (Accession # AJ012542.1, KJ147512.1, and HG424426.1, respectively).

Bottom Line: Phytosterol contents and food quality of plant produce is directly associated with transcription of gene squalene synthase (SS).Plant samples were analyzed through semi-quantitative PCR to evaluate effect of rhizobacterial application on transcriptional status of SS.The study would be helpful to improve quality of plant produce by modulating transcription of SS genes.

View Article: PubMed Central - PubMed

Affiliation: College of Earth and Environmental Sciences, University of the Punjab, Lahore Pakistan.

ABSTRACT
Phytosterol contents and food quality of plant produce is directly associated with transcription of gene squalene synthase (SS). In current study, barley plants were treated with different rhizobacterial strains under semi controlled (27 ± 3°C) greenhouse conditions in order to modulate expression of SS gene. Plant samples were analyzed through semi-quantitative PCR to evaluate effect of rhizobacterial application on transcriptional status of SS. Results revealed that among four SS genes (i.e., SSA, SS1, SS2, and SS3), the most expressive gene was SSA; while, SS2 was screened out as the second best induced gene due to Acetobacter aceti. The most efficient bacterial strain which recorded maximum gene expression was A. aceti AC8. Moreover, AC7 was reported as the least efficient bacterial species for inducing SS gene expression. AC8 enhanced the share of SSA and SS2 up to 43 and 31%, respectively. The study also described ribosomal sequence of the most efficient bacterial strain AC8, which was used to determine its phylogenetic relationships with other microbial strains. The study would be helpful to improve quality of plant produce by modulating transcription of SS genes.

No MeSH data available.


Related in: MedlinePlus