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Transcriptional modulation of squalene synthase genes in barley treated with PGPR.

Yousaf A, Qadir A, Anjum T, Ahmad A - Front Plant Sci (2015)

Bottom Line: Phytosterol contents and food quality of plant produce is directly associated with transcription of gene squalene synthase (SS).Plant samples were analyzed through semi-quantitative PCR to evaluate effect of rhizobacterial application on transcriptional status of SS.The study would be helpful to improve quality of plant produce by modulating transcription of SS genes.

View Article: PubMed Central - PubMed

Affiliation: College of Earth and Environmental Sciences, University of the Punjab, Lahore Pakistan.

ABSTRACT
Phytosterol contents and food quality of plant produce is directly associated with transcription of gene squalene synthase (SS). In current study, barley plants were treated with different rhizobacterial strains under semi controlled (27 ± 3°C) greenhouse conditions in order to modulate expression of SS gene. Plant samples were analyzed through semi-quantitative PCR to evaluate effect of rhizobacterial application on transcriptional status of SS. Results revealed that among four SS genes (i.e., SSA, SS1, SS2, and SS3), the most expressive gene was SSA; while, SS2 was screened out as the second best induced gene due to Acetobacter aceti. The most efficient bacterial strain which recorded maximum gene expression was A. aceti AC8. Moreover, AC7 was reported as the least efficient bacterial species for inducing SS gene expression. AC8 enhanced the share of SSA and SS2 up to 43 and 31%, respectively. The study also described ribosomal sequence of the most efficient bacterial strain AC8, which was used to determine its phylogenetic relationships with other microbial strains. The study would be helpful to improve quality of plant produce by modulating transcription of SS genes.

No MeSH data available.


Related in: MedlinePlus

Percentage share of SS genes (i.e., SSA, SS1, SS2, and SS3) in synthesis of total Squalene in Barley. Squalene share was estimated in control treatment of Barley (A) and in Barley plants treated with bacterial strain AC8 (B). Statistical package DSAASTAT (Onofri, Italy) was used to analyze data.
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Figure 5: Percentage share of SS genes (i.e., SSA, SS1, SS2, and SS3) in synthesis of total Squalene in Barley. Squalene share was estimated in control treatment of Barley (A) and in Barley plants treated with bacterial strain AC8 (B). Statistical package DSAASTAT (Onofri, Italy) was used to analyze data.

Mentions: In control treatment of barley, plants showed varied expression of SS genes. The most expressed gene was SS1 (35%) followed by equally expressed genes of SS2 and SSA, each having a share of 24%. Gene SS3 recorded the least percentage transcriptional share of 17%. Plants treated with strain AC8 exhibited gene SSA occupying maximum percentage share of SS (43%) in its transcriptional profile. Second most expressed gene was SS2 possessing 31% share of total SS transcription. Gene SS3 occupied the third position among transcriptional index of all the tested SS genes with its share of 20%. However, gene SS1 was the least expressed gene (6%) among all SS genes. Therefore, gene SS1 possessed the maximum expression (35%) and gene SS3 showed the least (17%) in control treatment. Whereas, gene SSA recorded the highest transcriptional share of 43% and gene SS1 recorded the least share 6% among treated plants (Figure 5).


Transcriptional modulation of squalene synthase genes in barley treated with PGPR.

Yousaf A, Qadir A, Anjum T, Ahmad A - Front Plant Sci (2015)

Percentage share of SS genes (i.e., SSA, SS1, SS2, and SS3) in synthesis of total Squalene in Barley. Squalene share was estimated in control treatment of Barley (A) and in Barley plants treated with bacterial strain AC8 (B). Statistical package DSAASTAT (Onofri, Italy) was used to analyze data.
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Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4555044&req=5

Figure 5: Percentage share of SS genes (i.e., SSA, SS1, SS2, and SS3) in synthesis of total Squalene in Barley. Squalene share was estimated in control treatment of Barley (A) and in Barley plants treated with bacterial strain AC8 (B). Statistical package DSAASTAT (Onofri, Italy) was used to analyze data.
Mentions: In control treatment of barley, plants showed varied expression of SS genes. The most expressed gene was SS1 (35%) followed by equally expressed genes of SS2 and SSA, each having a share of 24%. Gene SS3 recorded the least percentage transcriptional share of 17%. Plants treated with strain AC8 exhibited gene SSA occupying maximum percentage share of SS (43%) in its transcriptional profile. Second most expressed gene was SS2 possessing 31% share of total SS transcription. Gene SS3 occupied the third position among transcriptional index of all the tested SS genes with its share of 20%. However, gene SS1 was the least expressed gene (6%) among all SS genes. Therefore, gene SS1 possessed the maximum expression (35%) and gene SS3 showed the least (17%) in control treatment. Whereas, gene SSA recorded the highest transcriptional share of 43% and gene SS1 recorded the least share 6% among treated plants (Figure 5).

Bottom Line: Phytosterol contents and food quality of plant produce is directly associated with transcription of gene squalene synthase (SS).Plant samples were analyzed through semi-quantitative PCR to evaluate effect of rhizobacterial application on transcriptional status of SS.The study would be helpful to improve quality of plant produce by modulating transcription of SS genes.

View Article: PubMed Central - PubMed

Affiliation: College of Earth and Environmental Sciences, University of the Punjab, Lahore Pakistan.

ABSTRACT
Phytosterol contents and food quality of plant produce is directly associated with transcription of gene squalene synthase (SS). In current study, barley plants were treated with different rhizobacterial strains under semi controlled (27 ± 3°C) greenhouse conditions in order to modulate expression of SS gene. Plant samples were analyzed through semi-quantitative PCR to evaluate effect of rhizobacterial application on transcriptional status of SS. Results revealed that among four SS genes (i.e., SSA, SS1, SS2, and SS3), the most expressive gene was SSA; while, SS2 was screened out as the second best induced gene due to Acetobacter aceti. The most efficient bacterial strain which recorded maximum gene expression was A. aceti AC8. Moreover, AC7 was reported as the least efficient bacterial species for inducing SS gene expression. AC8 enhanced the share of SSA and SS2 up to 43 and 31%, respectively. The study also described ribosomal sequence of the most efficient bacterial strain AC8, which was used to determine its phylogenetic relationships with other microbial strains. The study would be helpful to improve quality of plant produce by modulating transcription of SS genes.

No MeSH data available.


Related in: MedlinePlus