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Transcriptional modulation of squalene synthase genes in barley treated with PGPR.

Yousaf A, Qadir A, Anjum T, Ahmad A - Front Plant Sci (2015)

Bottom Line: Phytosterol contents and food quality of plant produce is directly associated with transcription of gene squalene synthase (SS).Plant samples were analyzed through semi-quantitative PCR to evaluate effect of rhizobacterial application on transcriptional status of SS.The study would be helpful to improve quality of plant produce by modulating transcription of SS genes.

View Article: PubMed Central - PubMed

Affiliation: College of Earth and Environmental Sciences, University of the Punjab, Lahore Pakistan.

ABSTRACT
Phytosterol contents and food quality of plant produce is directly associated with transcription of gene squalene synthase (SS). In current study, barley plants were treated with different rhizobacterial strains under semi controlled (27 ± 3°C) greenhouse conditions in order to modulate expression of SS gene. Plant samples were analyzed through semi-quantitative PCR to evaluate effect of rhizobacterial application on transcriptional status of SS. Results revealed that among four SS genes (i.e., SSA, SS1, SS2, and SS3), the most expressive gene was SSA; while, SS2 was screened out as the second best induced gene due to Acetobacter aceti. The most efficient bacterial strain which recorded maximum gene expression was A. aceti AC8. Moreover, AC7 was reported as the least efficient bacterial species for inducing SS gene expression. AC8 enhanced the share of SSA and SS2 up to 43 and 31%, respectively. The study also described ribosomal sequence of the most efficient bacterial strain AC8, which was used to determine its phylogenetic relationships with other microbial strains. The study would be helpful to improve quality of plant produce by modulating transcription of SS genes.

No MeSH data available.


Related in: MedlinePlus

Efficiency of eight bacterial strains (i.e., AC1, AC2, AC3…. AC8) to induce transcription of SS in Barley plants. Data were statistically analysed through ANOVA and DMRT at p ≤ 0.05. Statistical analyses were performed at p ≤ 0.05 using Add-on package of MS-Excel, DSAASTAT (Onofri, Italy).
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Figure 4: Efficiency of eight bacterial strains (i.e., AC1, AC2, AC3…. AC8) to induce transcription of SS in Barley plants. Data were statistically analysed through ANOVA and DMRT at p ≤ 0.05. Statistical analyses were performed at p ≤ 0.05 using Add-on package of MS-Excel, DSAASTAT (Onofri, Italy).

Mentions: Maximum expression enhancement of SS genes (156.56%) was recorded due to application of treatment AC8. Strain AC4 triggered insignificantly lower expression (142.89%) of SS genes than strain AC8. Whereas, strains AC1 and AC3 were third among Squalene inducers’ index due to insignificantly different upregulation rates of 130.05 and 118.72%, respectively. Strains AC2, AC6, and AC7 showed significantly lower induction of SS genes product than strain AC3 with insignificant difference among them (i.e., 108.75, 106.80, and 102.24%, respectively). The least elevation in quantity of SS genes product was recorded due to application of treatment AC5 with numeric value of 82.67% (Figure 4).


Transcriptional modulation of squalene synthase genes in barley treated with PGPR.

Yousaf A, Qadir A, Anjum T, Ahmad A - Front Plant Sci (2015)

Efficiency of eight bacterial strains (i.e., AC1, AC2, AC3…. AC8) to induce transcription of SS in Barley plants. Data were statistically analysed through ANOVA and DMRT at p ≤ 0.05. Statistical analyses were performed at p ≤ 0.05 using Add-on package of MS-Excel, DSAASTAT (Onofri, Italy).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4555044&req=5

Figure 4: Efficiency of eight bacterial strains (i.e., AC1, AC2, AC3…. AC8) to induce transcription of SS in Barley plants. Data were statistically analysed through ANOVA and DMRT at p ≤ 0.05. Statistical analyses were performed at p ≤ 0.05 using Add-on package of MS-Excel, DSAASTAT (Onofri, Italy).
Mentions: Maximum expression enhancement of SS genes (156.56%) was recorded due to application of treatment AC8. Strain AC4 triggered insignificantly lower expression (142.89%) of SS genes than strain AC8. Whereas, strains AC1 and AC3 were third among Squalene inducers’ index due to insignificantly different upregulation rates of 130.05 and 118.72%, respectively. Strains AC2, AC6, and AC7 showed significantly lower induction of SS genes product than strain AC3 with insignificant difference among them (i.e., 108.75, 106.80, and 102.24%, respectively). The least elevation in quantity of SS genes product was recorded due to application of treatment AC5 with numeric value of 82.67% (Figure 4).

Bottom Line: Phytosterol contents and food quality of plant produce is directly associated with transcription of gene squalene synthase (SS).Plant samples were analyzed through semi-quantitative PCR to evaluate effect of rhizobacterial application on transcriptional status of SS.The study would be helpful to improve quality of plant produce by modulating transcription of SS genes.

View Article: PubMed Central - PubMed

Affiliation: College of Earth and Environmental Sciences, University of the Punjab, Lahore Pakistan.

ABSTRACT
Phytosterol contents and food quality of plant produce is directly associated with transcription of gene squalene synthase (SS). In current study, barley plants were treated with different rhizobacterial strains under semi controlled (27 ± 3°C) greenhouse conditions in order to modulate expression of SS gene. Plant samples were analyzed through semi-quantitative PCR to evaluate effect of rhizobacterial application on transcriptional status of SS. Results revealed that among four SS genes (i.e., SSA, SS1, SS2, and SS3), the most expressive gene was SSA; while, SS2 was screened out as the second best induced gene due to Acetobacter aceti. The most efficient bacterial strain which recorded maximum gene expression was A. aceti AC8. Moreover, AC7 was reported as the least efficient bacterial species for inducing SS gene expression. AC8 enhanced the share of SSA and SS2 up to 43 and 31%, respectively. The study also described ribosomal sequence of the most efficient bacterial strain AC8, which was used to determine its phylogenetic relationships with other microbial strains. The study would be helpful to improve quality of plant produce by modulating transcription of SS genes.

No MeSH data available.


Related in: MedlinePlus