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Transcriptional modulation of squalene synthase genes in barley treated with PGPR.

Yousaf A, Qadir A, Anjum T, Ahmad A - Front Plant Sci (2015)

Bottom Line: Phytosterol contents and food quality of plant produce is directly associated with transcription of gene squalene synthase (SS).Plant samples were analyzed through semi-quantitative PCR to evaluate effect of rhizobacterial application on transcriptional status of SS.The study would be helpful to improve quality of plant produce by modulating transcription of SS genes.

View Article: PubMed Central - PubMed

Affiliation: College of Earth and Environmental Sciences, University of the Punjab, Lahore Pakistan.

ABSTRACT
Phytosterol contents and food quality of plant produce is directly associated with transcription of gene squalene synthase (SS). In current study, barley plants were treated with different rhizobacterial strains under semi controlled (27 ± 3°C) greenhouse conditions in order to modulate expression of SS gene. Plant samples were analyzed through semi-quantitative PCR to evaluate effect of rhizobacterial application on transcriptional status of SS. Results revealed that among four SS genes (i.e., SSA, SS1, SS2, and SS3), the most expressive gene was SSA; while, SS2 was screened out as the second best induced gene due to Acetobacter aceti. The most efficient bacterial strain which recorded maximum gene expression was A. aceti AC8. Moreover, AC7 was reported as the least efficient bacterial species for inducing SS gene expression. AC8 enhanced the share of SSA and SS2 up to 43 and 31%, respectively. The study also described ribosomal sequence of the most efficient bacterial strain AC8, which was used to determine its phylogenetic relationships with other microbial strains. The study would be helpful to improve quality of plant produce by modulating transcription of SS genes.

No MeSH data available.


Related in: MedlinePlus

Percentage change in expression of four SS genes (i.e., SSA, SS1, SS2, and SS3) due to application of eight bacterial strains (AC1, AC2, AC3…… AC8). Data were statistically analysed through ANOVA and DMRT at p ≤ 0.05 using DSAASTAT (Onofri, Italy). Error bars show the SE calculated against each bar and significance of data has been mentioned through alphabets (e.g., a, b, c……) calculated at p ≤ 0.05.
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Figure 3: Percentage change in expression of four SS genes (i.e., SSA, SS1, SS2, and SS3) due to application of eight bacterial strains (AC1, AC2, AC3…… AC8). Data were statistically analysed through ANOVA and DMRT at p ≤ 0.05 using DSAASTAT (Onofri, Italy). Error bars show the SE calculated against each bar and significance of data has been mentioned through alphabets (e.g., a, b, c……) calculated at p ≤ 0.05.

Mentions: Transcriptional rate of gene SS1 did not elevated by any of the eight bacterial strains. Gene SS1 had not shown percentage elevation of its expression in any of the eight bacterial strains. Thus, it was recoded that percentage change of expression of gene SSA was maximum under influence of strain AC8 (Figure 3).


Transcriptional modulation of squalene synthase genes in barley treated with PGPR.

Yousaf A, Qadir A, Anjum T, Ahmad A - Front Plant Sci (2015)

Percentage change in expression of four SS genes (i.e., SSA, SS1, SS2, and SS3) due to application of eight bacterial strains (AC1, AC2, AC3…… AC8). Data were statistically analysed through ANOVA and DMRT at p ≤ 0.05 using DSAASTAT (Onofri, Italy). Error bars show the SE calculated against each bar and significance of data has been mentioned through alphabets (e.g., a, b, c……) calculated at p ≤ 0.05.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4555044&req=5

Figure 3: Percentage change in expression of four SS genes (i.e., SSA, SS1, SS2, and SS3) due to application of eight bacterial strains (AC1, AC2, AC3…… AC8). Data were statistically analysed through ANOVA and DMRT at p ≤ 0.05 using DSAASTAT (Onofri, Italy). Error bars show the SE calculated against each bar and significance of data has been mentioned through alphabets (e.g., a, b, c……) calculated at p ≤ 0.05.
Mentions: Transcriptional rate of gene SS1 did not elevated by any of the eight bacterial strains. Gene SS1 had not shown percentage elevation of its expression in any of the eight bacterial strains. Thus, it was recoded that percentage change of expression of gene SSA was maximum under influence of strain AC8 (Figure 3).

Bottom Line: Phytosterol contents and food quality of plant produce is directly associated with transcription of gene squalene synthase (SS).Plant samples were analyzed through semi-quantitative PCR to evaluate effect of rhizobacterial application on transcriptional status of SS.The study would be helpful to improve quality of plant produce by modulating transcription of SS genes.

View Article: PubMed Central - PubMed

Affiliation: College of Earth and Environmental Sciences, University of the Punjab, Lahore Pakistan.

ABSTRACT
Phytosterol contents and food quality of plant produce is directly associated with transcription of gene squalene synthase (SS). In current study, barley plants were treated with different rhizobacterial strains under semi controlled (27 ± 3°C) greenhouse conditions in order to modulate expression of SS gene. Plant samples were analyzed through semi-quantitative PCR to evaluate effect of rhizobacterial application on transcriptional status of SS. Results revealed that among four SS genes (i.e., SSA, SS1, SS2, and SS3), the most expressive gene was SSA; while, SS2 was screened out as the second best induced gene due to Acetobacter aceti. The most efficient bacterial strain which recorded maximum gene expression was A. aceti AC8. Moreover, AC7 was reported as the least efficient bacterial species for inducing SS gene expression. AC8 enhanced the share of SSA and SS2 up to 43 and 31%, respectively. The study also described ribosomal sequence of the most efficient bacterial strain AC8, which was used to determine its phylogenetic relationships with other microbial strains. The study would be helpful to improve quality of plant produce by modulating transcription of SS genes.

No MeSH data available.


Related in: MedlinePlus