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Transcriptional modulation of squalene synthase genes in barley treated with PGPR.

Yousaf A, Qadir A, Anjum T, Ahmad A - Front Plant Sci (2015)

Bottom Line: Phytosterol contents and food quality of plant produce is directly associated with transcription of gene squalene synthase (SS).Plant samples were analyzed through semi-quantitative PCR to evaluate effect of rhizobacterial application on transcriptional status of SS.The study would be helpful to improve quality of plant produce by modulating transcription of SS genes.

View Article: PubMed Central - PubMed

Affiliation: College of Earth and Environmental Sciences, University of the Punjab, Lahore Pakistan.

ABSTRACT
Phytosterol contents and food quality of plant produce is directly associated with transcription of gene squalene synthase (SS). In current study, barley plants were treated with different rhizobacterial strains under semi controlled (27 ± 3°C) greenhouse conditions in order to modulate expression of SS gene. Plant samples were analyzed through semi-quantitative PCR to evaluate effect of rhizobacterial application on transcriptional status of SS. Results revealed that among four SS genes (i.e., SSA, SS1, SS2, and SS3), the most expressive gene was SSA; while, SS2 was screened out as the second best induced gene due to Acetobacter aceti. The most efficient bacterial strain which recorded maximum gene expression was A. aceti AC8. Moreover, AC7 was reported as the least efficient bacterial species for inducing SS gene expression. AC8 enhanced the share of SSA and SS2 up to 43 and 31%, respectively. The study also described ribosomal sequence of the most efficient bacterial strain AC8, which was used to determine its phylogenetic relationships with other microbial strains. The study would be helpful to improve quality of plant produce by modulating transcription of SS genes.

No MeSH data available.


Related in: MedlinePlus

Agarose gel image of RT-PCR product of four squalene synthase (SS) genes (i.e., SSA, SS1, SS2, and SS3) detected in Barley plants. Barley was treated with eight bacterial strains (i.e., AC1, AC2, AC3…. AC8) to evaluate their effect on expression of SS genes. DNA Marker (DNA Ladder) was loaded in a well to compare it with other bands as a standard reference.
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Figure 1: Agarose gel image of RT-PCR product of four squalene synthase (SS) genes (i.e., SSA, SS1, SS2, and SS3) detected in Barley plants. Barley was treated with eight bacterial strains (i.e., AC1, AC2, AC3…. AC8) to evaluate their effect on expression of SS genes. DNA Marker (DNA Ladder) was loaded in a well to compare it with other bands as a standard reference.

Mentions: RT-PCR results revealed that total four types of SS genes were expressed in barley (i.e., SSA, SS1, SS2, and SS3). All these four genes exhibited different expression levels. AC8 was screened out as the best bacterial inducer, which showed maximum activity for elevation of gene expression and phytosterol contents. The most expressive gene recorded in treatment AC8 was SSA, which showed its maximum role in production of SS, and concomitantly in phytosterols production. Furthermore, SS2 was screened out as a second highly induced gene by applying treatment AC8. SS3 was screened out as the best induced gene at third level. SS1 was reported as the least expressive gene. The most favorable bacterial strain which exhibited the best relationship with barley and reported maximum gene expression was AC8. AC7 was reported as the least significant bacterial strain in expression of gene SS1 (Figure 1).


Transcriptional modulation of squalene synthase genes in barley treated with PGPR.

Yousaf A, Qadir A, Anjum T, Ahmad A - Front Plant Sci (2015)

Agarose gel image of RT-PCR product of four squalene synthase (SS) genes (i.e., SSA, SS1, SS2, and SS3) detected in Barley plants. Barley was treated with eight bacterial strains (i.e., AC1, AC2, AC3…. AC8) to evaluate their effect on expression of SS genes. DNA Marker (DNA Ladder) was loaded in a well to compare it with other bands as a standard reference.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4555044&req=5

Figure 1: Agarose gel image of RT-PCR product of four squalene synthase (SS) genes (i.e., SSA, SS1, SS2, and SS3) detected in Barley plants. Barley was treated with eight bacterial strains (i.e., AC1, AC2, AC3…. AC8) to evaluate their effect on expression of SS genes. DNA Marker (DNA Ladder) was loaded in a well to compare it with other bands as a standard reference.
Mentions: RT-PCR results revealed that total four types of SS genes were expressed in barley (i.e., SSA, SS1, SS2, and SS3). All these four genes exhibited different expression levels. AC8 was screened out as the best bacterial inducer, which showed maximum activity for elevation of gene expression and phytosterol contents. The most expressive gene recorded in treatment AC8 was SSA, which showed its maximum role in production of SS, and concomitantly in phytosterols production. Furthermore, SS2 was screened out as a second highly induced gene by applying treatment AC8. SS3 was screened out as the best induced gene at third level. SS1 was reported as the least expressive gene. The most favorable bacterial strain which exhibited the best relationship with barley and reported maximum gene expression was AC8. AC7 was reported as the least significant bacterial strain in expression of gene SS1 (Figure 1).

Bottom Line: Phytosterol contents and food quality of plant produce is directly associated with transcription of gene squalene synthase (SS).Plant samples were analyzed through semi-quantitative PCR to evaluate effect of rhizobacterial application on transcriptional status of SS.The study would be helpful to improve quality of plant produce by modulating transcription of SS genes.

View Article: PubMed Central - PubMed

Affiliation: College of Earth and Environmental Sciences, University of the Punjab, Lahore Pakistan.

ABSTRACT
Phytosterol contents and food quality of plant produce is directly associated with transcription of gene squalene synthase (SS). In current study, barley plants were treated with different rhizobacterial strains under semi controlled (27 ± 3°C) greenhouse conditions in order to modulate expression of SS gene. Plant samples were analyzed through semi-quantitative PCR to evaluate effect of rhizobacterial application on transcriptional status of SS. Results revealed that among four SS genes (i.e., SSA, SS1, SS2, and SS3), the most expressive gene was SSA; while, SS2 was screened out as the second best induced gene due to Acetobacter aceti. The most efficient bacterial strain which recorded maximum gene expression was A. aceti AC8. Moreover, AC7 was reported as the least efficient bacterial species for inducing SS gene expression. AC8 enhanced the share of SSA and SS2 up to 43 and 31%, respectively. The study also described ribosomal sequence of the most efficient bacterial strain AC8, which was used to determine its phylogenetic relationships with other microbial strains. The study would be helpful to improve quality of plant produce by modulating transcription of SS genes.

No MeSH data available.


Related in: MedlinePlus