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2B4 Is Dispensable for T-Dependent B Cell Immune Responses, but Its Deficiency Leads to Enhanced T-Independent Responses Due to an Increase in Peritoneal Cavity B1b Cells.

Ray A, Yuan CY, Miller NM, Mei H, Dittel BN - PLoS ONE (2015)

Bottom Line: We found that the splenic cellularity in Cd244-/- mice was significantly reduced due to a reduction in both CD4 T cells and follicular (Fo) B cells; whereas, the number of peritoneal cavity B cells was increased.When we evaluated T-independent immune responses, we found that antigen-specific IgM and IgG3 were elevated in the serum following immunization.These data indicate that 2B4 dampens T-independent B cell responses due to a reduction in peritoneal cavity B cells, but has minimal impact on T-dependent B cell responses.

View Article: PubMed Central - PubMed

Affiliation: Blood Research Institute, BloodCenter of Wisconsin, Milwaukee, Wisconsin, United States of America.

ABSTRACT
The signaling lymphocyte activation molecule (SLAM) family plays important roles in adaptive immune responses. Herein, we evaluated whether the SLAM family member 2B4 (CD244) plays a role in immune cell development, homeostasis and antibody responses. We found that the splenic cellularity in Cd244-/- mice was significantly reduced due to a reduction in both CD4 T cells and follicular (Fo) B cells; whereas, the number of peritoneal cavity B cells was increased. These findings led us to examine whether 2B4 modulates B cell immune responses. When we examined T-dependent B cell responses, while there was no difference in the kinetics or magnitude of the antigen-specific IgM and IgG1 antibody response there was a reduction in bone marrow (BM) memory, but not plasma cells in Cd244-/- mice. When we evaluated T-independent immune responses, we found that antigen-specific IgM and IgG3 were elevated in the serum following immunization. These data indicate that 2B4 dampens T-independent B cell responses due to a reduction in peritoneal cavity B cells, but has minimal impact on T-dependent B cell responses.

No MeSH data available.


Splenic B cells express low levels of 2B4.Expression of 2B4 was evaluated on splenic NK cells (NK1.1+), B cells (B220+), Fo B cells and MZ B cells from WT and Cd244-/- mice. Histograms display overlaid staining profiles from the biotin negative control (shaded), WT mice (thin line) and Cd244-/- mice (thick line). Data shown are from one representative mouse of two.
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pone.0137314.g003: Splenic B cells express low levels of 2B4.Expression of 2B4 was evaluated on splenic NK cells (NK1.1+), B cells (B220+), Fo B cells and MZ B cells from WT and Cd244-/- mice. Histograms display overlaid staining profiles from the biotin negative control (shaded), WT mice (thin line) and Cd244-/- mice (thick line). Data shown are from one representative mouse of two.

Mentions: We next determined whether splenic B cell subsets express 2B4 in the steady state by flow cytomtery. Because the lack of reported 2B4 expression by B cells could be due to a low expression level, we used a three step staining procedure to amplify the 2B4 signal. In addition, we performed the same staining in Cd244-/- mice to confirm specificity. As a positive control, we analyzed 2B4 expression on NK cells, which expressed 2B4 at a high level (Fig 3). 2B4 cell surface expression by flow cytometry was not evident on either the total B220+ or Fo subset (Fig 3). However, we found that MZ B cells express 2B4 at a very low level (Fig 3).


2B4 Is Dispensable for T-Dependent B Cell Immune Responses, but Its Deficiency Leads to Enhanced T-Independent Responses Due to an Increase in Peritoneal Cavity B1b Cells.

Ray A, Yuan CY, Miller NM, Mei H, Dittel BN - PLoS ONE (2015)

Splenic B cells express low levels of 2B4.Expression of 2B4 was evaluated on splenic NK cells (NK1.1+), B cells (B220+), Fo B cells and MZ B cells from WT and Cd244-/- mice. Histograms display overlaid staining profiles from the biotin negative control (shaded), WT mice (thin line) and Cd244-/- mice (thick line). Data shown are from one representative mouse of two.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4554987&req=5

pone.0137314.g003: Splenic B cells express low levels of 2B4.Expression of 2B4 was evaluated on splenic NK cells (NK1.1+), B cells (B220+), Fo B cells and MZ B cells from WT and Cd244-/- mice. Histograms display overlaid staining profiles from the biotin negative control (shaded), WT mice (thin line) and Cd244-/- mice (thick line). Data shown are from one representative mouse of two.
Mentions: We next determined whether splenic B cell subsets express 2B4 in the steady state by flow cytomtery. Because the lack of reported 2B4 expression by B cells could be due to a low expression level, we used a three step staining procedure to amplify the 2B4 signal. In addition, we performed the same staining in Cd244-/- mice to confirm specificity. As a positive control, we analyzed 2B4 expression on NK cells, which expressed 2B4 at a high level (Fig 3). 2B4 cell surface expression by flow cytometry was not evident on either the total B220+ or Fo subset (Fig 3). However, we found that MZ B cells express 2B4 at a very low level (Fig 3).

Bottom Line: We found that the splenic cellularity in Cd244-/- mice was significantly reduced due to a reduction in both CD4 T cells and follicular (Fo) B cells; whereas, the number of peritoneal cavity B cells was increased.When we evaluated T-independent immune responses, we found that antigen-specific IgM and IgG3 were elevated in the serum following immunization.These data indicate that 2B4 dampens T-independent B cell responses due to a reduction in peritoneal cavity B cells, but has minimal impact on T-dependent B cell responses.

View Article: PubMed Central - PubMed

Affiliation: Blood Research Institute, BloodCenter of Wisconsin, Milwaukee, Wisconsin, United States of America.

ABSTRACT
The signaling lymphocyte activation molecule (SLAM) family plays important roles in adaptive immune responses. Herein, we evaluated whether the SLAM family member 2B4 (CD244) plays a role in immune cell development, homeostasis and antibody responses. We found that the splenic cellularity in Cd244-/- mice was significantly reduced due to a reduction in both CD4 T cells and follicular (Fo) B cells; whereas, the number of peritoneal cavity B cells was increased. These findings led us to examine whether 2B4 modulates B cell immune responses. When we examined T-dependent B cell responses, while there was no difference in the kinetics or magnitude of the antigen-specific IgM and IgG1 antibody response there was a reduction in bone marrow (BM) memory, but not plasma cells in Cd244-/- mice. When we evaluated T-independent immune responses, we found that antigen-specific IgM and IgG3 were elevated in the serum following immunization. These data indicate that 2B4 dampens T-independent B cell responses due to a reduction in peritoneal cavity B cells, but has minimal impact on T-dependent B cell responses.

No MeSH data available.