Limits...
Electrochemotherapy of tumors as in situ vaccination boosted by immunogene electrotransfer.

Sersa G, Teissie J, Cemazar M, Signori E, Kamensek U, Marshall G, Miklavcic D - Cancer Immunol. Immunother. (2015)

Bottom Line: The therapeutic effectiveness of delivered chemotherapeutics or nucleic acids depends greatly on their successful and efficient delivery to the target tissue.In an attempt to increase systemic antitumor effectiveness of electrochemotherapy, electrotransfer of genes with immunomodulatory effect (immunogene electrotransfer) could be used as adjuvant treatment.Since electrochemotherapy can induce immunogenic cell death, adjuvant immunogene electrotransfer to peritumoral tissue could lead to locoregional effect as well as the abscopal effect on distant untreated metastases.

View Article: PubMed Central - PubMed

Affiliation: Department of Experimental Oncology, Institute of Oncology Ljubljana, Zaloska 2, 1000, Ljubljana, Slovenia, gsersa@onko-i.si.

ABSTRACT
Electroporation is a platform technology for drug and gene delivery. When applied to cell in vitro or tissues in vivo, it leads to an increase in membrane permeability for molecules which otherwise cannot enter the cell (e.g., siRNA, plasmid DNA, and some chemotherapeutic drugs). The therapeutic effectiveness of delivered chemotherapeutics or nucleic acids depends greatly on their successful and efficient delivery to the target tissue. Therefore, the understanding of different principles of drug and gene delivery is necessary and needs to be taken into account according to the specificity of their delivery to tumors and/or normal tissues. Based on the current knowledge, electrochemotherapy (a combination of drug and electric pulses) is used for tumor treatment and has shown great potential. Its local effectiveness is up to 80 % of local tumor control, however, without noticeable effect on metastases. In an attempt to increase systemic antitumor effectiveness of electrochemotherapy, electrotransfer of genes with immunomodulatory effect (immunogene electrotransfer) could be used as adjuvant treatment. Since electrochemotherapy can induce immunogenic cell death, adjuvant immunogene electrotransfer to peritumoral tissue could lead to locoregional effect as well as the abscopal effect on distant untreated metastases. Therefore, we propose a combination of electrochemotherapy with peritumoral IL-12 electrotransfer, as a proof of principle, using electrochemotherapy boosted with immunogene electrotransfer as in situ vaccination for successful tumor treatment.

Show MeSH

Related in: MedlinePlus

Schematic cartoon showing the processes occurring before, during, and after application of electric pulses (yellow stripe) for the delivery of molecules of different sizes into the cell. a Delivery of small molecules—an example of electrochemotherapy; b delivery of oligonucleotides—an example of siRNA electrotransfer; c delivery of larger nucleic acids/macromolecules—an example of pDNA gene electrotransfer
© Copyright Policy - OpenAccess
Related In: Results  -  Collection


getmorefigures.php?uid=PMC4554735&req=5

Fig1: Schematic cartoon showing the processes occurring before, during, and after application of electric pulses (yellow stripe) for the delivery of molecules of different sizes into the cell. a Delivery of small molecules—an example of electrochemotherapy; b delivery of oligonucleotides—an example of siRNA electrotransfer; c delivery of larger nucleic acids/macromolecules—an example of pDNA gene electrotransfer

Mentions: Electrophoretic contribution during the pulse remains negligible in the long-term loading process [31]. Molecular transfer of small molecules (<4 kDa) across the permeabilized area of the membrane is mostly driven by the concentration gradient across the membrane described by the Fick equation during the resealing process, i.e., after the pulse delivery [32] (Fig. 1).Fig. 1


Electrochemotherapy of tumors as in situ vaccination boosted by immunogene electrotransfer.

Sersa G, Teissie J, Cemazar M, Signori E, Kamensek U, Marshall G, Miklavcic D - Cancer Immunol. Immunother. (2015)

Schematic cartoon showing the processes occurring before, during, and after application of electric pulses (yellow stripe) for the delivery of molecules of different sizes into the cell. a Delivery of small molecules—an example of electrochemotherapy; b delivery of oligonucleotides—an example of siRNA electrotransfer; c delivery of larger nucleic acids/macromolecules—an example of pDNA gene electrotransfer
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4554735&req=5

Fig1: Schematic cartoon showing the processes occurring before, during, and after application of electric pulses (yellow stripe) for the delivery of molecules of different sizes into the cell. a Delivery of small molecules—an example of electrochemotherapy; b delivery of oligonucleotides—an example of siRNA electrotransfer; c delivery of larger nucleic acids/macromolecules—an example of pDNA gene electrotransfer
Mentions: Electrophoretic contribution during the pulse remains negligible in the long-term loading process [31]. Molecular transfer of small molecules (<4 kDa) across the permeabilized area of the membrane is mostly driven by the concentration gradient across the membrane described by the Fick equation during the resealing process, i.e., after the pulse delivery [32] (Fig. 1).Fig. 1

Bottom Line: The therapeutic effectiveness of delivered chemotherapeutics or nucleic acids depends greatly on their successful and efficient delivery to the target tissue.In an attempt to increase systemic antitumor effectiveness of electrochemotherapy, electrotransfer of genes with immunomodulatory effect (immunogene electrotransfer) could be used as adjuvant treatment.Since electrochemotherapy can induce immunogenic cell death, adjuvant immunogene electrotransfer to peritumoral tissue could lead to locoregional effect as well as the abscopal effect on distant untreated metastases.

View Article: PubMed Central - PubMed

Affiliation: Department of Experimental Oncology, Institute of Oncology Ljubljana, Zaloska 2, 1000, Ljubljana, Slovenia, gsersa@onko-i.si.

ABSTRACT
Electroporation is a platform technology for drug and gene delivery. When applied to cell in vitro or tissues in vivo, it leads to an increase in membrane permeability for molecules which otherwise cannot enter the cell (e.g., siRNA, plasmid DNA, and some chemotherapeutic drugs). The therapeutic effectiveness of delivered chemotherapeutics or nucleic acids depends greatly on their successful and efficient delivery to the target tissue. Therefore, the understanding of different principles of drug and gene delivery is necessary and needs to be taken into account according to the specificity of their delivery to tumors and/or normal tissues. Based on the current knowledge, electrochemotherapy (a combination of drug and electric pulses) is used for tumor treatment and has shown great potential. Its local effectiveness is up to 80 % of local tumor control, however, without noticeable effect on metastases. In an attempt to increase systemic antitumor effectiveness of electrochemotherapy, electrotransfer of genes with immunomodulatory effect (immunogene electrotransfer) could be used as adjuvant treatment. Since electrochemotherapy can induce immunogenic cell death, adjuvant immunogene electrotransfer to peritumoral tissue could lead to locoregional effect as well as the abscopal effect on distant untreated metastases. Therefore, we propose a combination of electrochemotherapy with peritumoral IL-12 electrotransfer, as a proof of principle, using electrochemotherapy boosted with immunogene electrotransfer as in situ vaccination for successful tumor treatment.

Show MeSH
Related in: MedlinePlus