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Biogas production using anaerobic groundwater containing a subterranean microbial community associated with the accretionary prism.

Baito K, Imai S, Matsushita M, Otani M, Sato Y, Kimura H - Microb Biotechnol (2014)

Bottom Line: In a deep aquifer associated with an accretionary prism, significant methane (CH₄) is produced by a subterranean microbial community.After the H₂ decreased, rapid CH₄ production was observed.The results suggested that syntrophic biodegradation of organic substrates by the H₂ -producing fermentative bacterium and the hydrogenotrophic methanogen contributed to the CH₄ production.

View Article: PubMed Central - PubMed

Affiliation: Department of Geosciences, Graduate School of Science, Shizuoka University, Shizuoka, Japan.

No MeSH data available.


Scanning electron micrographs of microbial cells derived from the reactor for H2 production. Bar, 1 μm.
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fig05: Scanning electron micrographs of microbial cells derived from the reactor for H2 production. Bar, 1 μm.

Mentions: Scanning electron microscopy showed the dominance of cocci and relatively thick, sausage-shaped cells were observed frequently in the culture fluid for H2 production (Fig. 5A). We did not find the long cells and the flagellum-like filaments that were observed in the reactor for CH4 production (Fig. 5B). In the phylogenetic analysis, archaeal 16S rRNA gene was not amplified from the bulk DNA after repeated PCR. These results suggested that hydrogenotrophic methanogens derived from anaerobic groundwater were completely inhibited by BES supplementation.


Biogas production using anaerobic groundwater containing a subterranean microbial community associated with the accretionary prism.

Baito K, Imai S, Matsushita M, Otani M, Sato Y, Kimura H - Microb Biotechnol (2014)

Scanning electron micrographs of microbial cells derived from the reactor for H2 production. Bar, 1 μm.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4554471&req=5

fig05: Scanning electron micrographs of microbial cells derived from the reactor for H2 production. Bar, 1 μm.
Mentions: Scanning electron microscopy showed the dominance of cocci and relatively thick, sausage-shaped cells were observed frequently in the culture fluid for H2 production (Fig. 5A). We did not find the long cells and the flagellum-like filaments that were observed in the reactor for CH4 production (Fig. 5B). In the phylogenetic analysis, archaeal 16S rRNA gene was not amplified from the bulk DNA after repeated PCR. These results suggested that hydrogenotrophic methanogens derived from anaerobic groundwater were completely inhibited by BES supplementation.

Bottom Line: In a deep aquifer associated with an accretionary prism, significant methane (CH₄) is produced by a subterranean microbial community.After the H₂ decreased, rapid CH₄ production was observed.The results suggested that syntrophic biodegradation of organic substrates by the H₂ -producing fermentative bacterium and the hydrogenotrophic methanogen contributed to the CH₄ production.

View Article: PubMed Central - PubMed

Affiliation: Department of Geosciences, Graduate School of Science, Shizuoka University, Shizuoka, Japan.

No MeSH data available.