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Biogas production using anaerobic groundwater containing a subterranean microbial community associated with the accretionary prism.

Baito K, Imai S, Matsushita M, Otani M, Sato Y, Kimura H - Microb Biotechnol (2014)

Bottom Line: In a deep aquifer associated with an accretionary prism, significant methane (CH₄) is produced by a subterranean microbial community.After the H₂ decreased, rapid CH₄ production was observed.The results suggested that syntrophic biodegradation of organic substrates by the H₂ -producing fermentative bacterium and the hydrogenotrophic methanogen contributed to the CH₄ production.

View Article: PubMed Central - PubMed

Affiliation: Department of Geosciences, Graduate School of Science, Shizuoka University, Shizuoka, Japan.

No MeSH data available.


Scanning electron micrographs of microbial cells derived from the reactor for CH4 production. Bar, 1 μm.
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fig03: Scanning electron micrographs of microbial cells derived from the reactor for CH4 production. Bar, 1 μm.

Mentions: Scanning electron microscopy showed the dominance of long, irregularly curved cells in the culture fluid for CH4 production (Fig. 3A). In addition to the long cells, relatively thick, rod-shaped cells were observed in the reactor. We also found flagellum-like filaments connecting the long cells and rod-shaped cells, particularly in large cell aggregates (Fig. 3B). Although the phylogenetic position of the long cells was not determined, they were considered likely to be the hydrogenotrophic methanogen M. thermautotrophicus based on their morphological characteristics (Zeikus and Wolfe, 1972). A previous study suggested that the flagellum-like filaments provided ‘interspecies electron/hydrogen transfer’ in a syntrophic consortium established by fermentative bacteria and methanogenic archaea (Ishii et al., 2005; Shimoyama et al., 2009; Hillesland and Stahl, 2010; Walker et al., 2012). The results underscore that the microbial syntrophy of T. syntrophicus and M. thermautotrophicus derived from the anaerobic groundwater contributes to the CH4 production in the bioreactor.


Biogas production using anaerobic groundwater containing a subterranean microbial community associated with the accretionary prism.

Baito K, Imai S, Matsushita M, Otani M, Sato Y, Kimura H - Microb Biotechnol (2014)

Scanning electron micrographs of microbial cells derived from the reactor for CH4 production. Bar, 1 μm.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4554471&req=5

fig03: Scanning electron micrographs of microbial cells derived from the reactor for CH4 production. Bar, 1 μm.
Mentions: Scanning electron microscopy showed the dominance of long, irregularly curved cells in the culture fluid for CH4 production (Fig. 3A). In addition to the long cells, relatively thick, rod-shaped cells were observed in the reactor. We also found flagellum-like filaments connecting the long cells and rod-shaped cells, particularly in large cell aggregates (Fig. 3B). Although the phylogenetic position of the long cells was not determined, they were considered likely to be the hydrogenotrophic methanogen M. thermautotrophicus based on their morphological characteristics (Zeikus and Wolfe, 1972). A previous study suggested that the flagellum-like filaments provided ‘interspecies electron/hydrogen transfer’ in a syntrophic consortium established by fermentative bacteria and methanogenic archaea (Ishii et al., 2005; Shimoyama et al., 2009; Hillesland and Stahl, 2010; Walker et al., 2012). The results underscore that the microbial syntrophy of T. syntrophicus and M. thermautotrophicus derived from the anaerobic groundwater contributes to the CH4 production in the bioreactor.

Bottom Line: In a deep aquifer associated with an accretionary prism, significant methane (CH₄) is produced by a subterranean microbial community.After the H₂ decreased, rapid CH₄ production was observed.The results suggested that syntrophic biodegradation of organic substrates by the H₂ -producing fermentative bacterium and the hydrogenotrophic methanogen contributed to the CH₄ production.

View Article: PubMed Central - PubMed

Affiliation: Department of Geosciences, Graduate School of Science, Shizuoka University, Shizuoka, Japan.

No MeSH data available.