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Biogas production using anaerobic groundwater containing a subterranean microbial community associated with the accretionary prism.

Baito K, Imai S, Matsushita M, Otani M, Sato Y, Kimura H - Microb Biotechnol (2014)

Bottom Line: In a deep aquifer associated with an accretionary prism, significant methane (CH₄) is produced by a subterranean microbial community.After the H₂ decreased, rapid CH₄ production was observed.The results suggested that syntrophic biodegradation of organic substrates by the H₂ -producing fermentative bacterium and the hydrogenotrophic methanogen contributed to the CH₄ production.

View Article: PubMed Central - PubMed

Affiliation: Department of Geosciences, Graduate School of Science, Shizuoka University, Shizuoka, Japan.

No MeSH data available.


Dynamics of the biogas and reactor in the bioreactor for CH4 production using anaerobic groundwater amended with 0.22% YPG: ▲, H2; ○, CH4; ×, CO2; ◇, pressure; □, pH; △, OD600; ●, acetate. Gray lines indicate YPG supplement and biogas removal by bubbling using pure N2.
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fig02: Dynamics of the biogas and reactor in the bioreactor for CH4 production using anaerobic groundwater amended with 0.22% YPG: ▲, H2; ○, CH4; ×, CO2; ◇, pressure; □, pH; △, OD600; ●, acetate. Gray lines indicate YPG supplement and biogas removal by bubbling using pure N2.

Mentions: The groundwater sample was anaerobically collected from the deep well and directly poured into the bioreactor (Fig. 1A) through the influent port using a sterile silicone tube (Fig. S3). In order to produce CH4, the anaerobic groundwater amended with yeast extract, peptone and glucose (YPG) medium was incubated at 55°C (Fig. 1B). Initially, H2 was detected within 24 h and accumulated in the gas phase of the bioreactor. Hydrogen then decreased to below the detection limit. Following the disappearance of the H2, CH4 production began to be observed. Methane increased to 46 mmol in the bioreactor amended with 0.22% YPG (Fig. 2) and 174 mmol in the bioreactor amended with 1.0% YPG (Fig. S4). The pressure of the gas phase was increased to 55.7 kPa and 129 kPa in the bioreactor supplemented with 0.22% YPG and 1.0% YPG respectively.


Biogas production using anaerobic groundwater containing a subterranean microbial community associated with the accretionary prism.

Baito K, Imai S, Matsushita M, Otani M, Sato Y, Kimura H - Microb Biotechnol (2014)

Dynamics of the biogas and reactor in the bioreactor for CH4 production using anaerobic groundwater amended with 0.22% YPG: ▲, H2; ○, CH4; ×, CO2; ◇, pressure; □, pH; △, OD600; ●, acetate. Gray lines indicate YPG supplement and biogas removal by bubbling using pure N2.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4554471&req=5

fig02: Dynamics of the biogas and reactor in the bioreactor for CH4 production using anaerobic groundwater amended with 0.22% YPG: ▲, H2; ○, CH4; ×, CO2; ◇, pressure; □, pH; △, OD600; ●, acetate. Gray lines indicate YPG supplement and biogas removal by bubbling using pure N2.
Mentions: The groundwater sample was anaerobically collected from the deep well and directly poured into the bioreactor (Fig. 1A) through the influent port using a sterile silicone tube (Fig. S3). In order to produce CH4, the anaerobic groundwater amended with yeast extract, peptone and glucose (YPG) medium was incubated at 55°C (Fig. 1B). Initially, H2 was detected within 24 h and accumulated in the gas phase of the bioreactor. Hydrogen then decreased to below the detection limit. Following the disappearance of the H2, CH4 production began to be observed. Methane increased to 46 mmol in the bioreactor amended with 0.22% YPG (Fig. 2) and 174 mmol in the bioreactor amended with 1.0% YPG (Fig. S4). The pressure of the gas phase was increased to 55.7 kPa and 129 kPa in the bioreactor supplemented with 0.22% YPG and 1.0% YPG respectively.

Bottom Line: In a deep aquifer associated with an accretionary prism, significant methane (CH₄) is produced by a subterranean microbial community.After the H₂ decreased, rapid CH₄ production was observed.The results suggested that syntrophic biodegradation of organic substrates by the H₂ -producing fermentative bacterium and the hydrogenotrophic methanogen contributed to the CH₄ production.

View Article: PubMed Central - PubMed

Affiliation: Department of Geosciences, Graduate School of Science, Shizuoka University, Shizuoka, Japan.

No MeSH data available.