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Characterization of microbial community structure during continuous anaerobic digestion of straw and cow manure.

Sun L, Pope PB, Eijsink VG, Schnürer A - Microb Biotechnol (2015)

Bottom Line: Compared with manure itself, digestion of manure resulted in a higher abundance of an uncultured class WWE1 and lower abundance of Bacilli.Adding straw to the digesters increased the level of Bacteroidia, while increasing the operating temperature decreased the level of this class and instead increased the relative abundance of an uncultured genus affiliated to order MBA08 (Clostridia).A considerable fraction of bacterial sequences could not be allocated to genus level, indicating that novel phylotypes are resident in these communities.

View Article: PubMed Central - PubMed

Affiliation: Department of Microbiology, Swedish University of Agricultural Science, Uppsala BioCenter, P.O. Box 7025, SE-750 07, Uppsala, Sweden.

No MeSH data available.


Relative abundance of bacterial 16S rRNA gene at the order level in manure (S1 and S2 correspond to duplicate samples) and in laboratory-scale digesters processing: (1) manure as sole substrate (RM); (2) stream-exploded straw and manure, operating constantly at 37°C (RTcSS); and (3) steam-exploded straw and manure, operating temperatures 37°C, 44°C and 52°C (R37SS, R44SS and R52SS), where R1 and R2 represent parallel digesters.
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fig04: Relative abundance of bacterial 16S rRNA gene at the order level in manure (S1 and S2 correspond to duplicate samples) and in laboratory-scale digesters processing: (1) manure as sole substrate (RM); (2) stream-exploded straw and manure, operating constantly at 37°C (RTcSS); and (3) steam-exploded straw and manure, operating temperatures 37°C, 44°C and 52°C (R37SS, R44SS and R52SS), where R1 and R2 represent parallel digesters.

Mentions: Firmicutes was observed in all digesters as well as in the manure samples, with the highest relative abundance in the digesters operated at 52°C (97.6–97.8%), followed by the digesters operated at 44°C (76.5–82.5%). In the remaining samples, the relative abundance of sequences belonging to this phylum was 56.8–74.1% (manure), 45.9–50.4% (RM, digester operated with manure alone) and 32.6–49.5% (R37SS and RTcSS, Fig. S3). Within the Firmicutes, sequences belonging to the classes Bacilli and Clostridia dominated (Fig. 3). The class Clostridia was represented in similar levels in the digesters operated at 37°C (26.8–41.9%) and in the manure samples (30.4–34.9%). However, in line with the increase in operating temperature, the relative abundance of sequences within this class increased and was 66.8–77.2% and 92.4–93.8% in R44SS and R52SS respectively. Within the class Clostridia, sequences belonging to the order Clostridiales and to the uncultured order MBA08 were found to be dominant (Fig. 4). Clostridiales was present in manure (29.4–33.7%) and all digesters (10.1–38.8) with the lowest relative abundance at 52°C (10.1–15.0%), while MBA08 was not detected in manure but in all digesters (1.9–74.4%) with the highest relative abundance at 52°C (67.1–74.4%). Within the order Clostridiales, the families Peptostreptococcaceae (5.4–18.1%) and Clostridiaceae (2.2–11.1%) were identified as the major groups. In addition, Lachnospiraceae, Ruminococcaceae, Syntrophomonadaceae, Peptococcaceae, Catabacteriaceae, Eubacteriaceae and Veillonellaceae were present at lower levels (data not shown). Within the Clostridiaceae, sequences belonging to the genera Clostridium and Sedimentibacter dominated (Fig. S2). Clostridium was detected in all sampling points (0.7–4.4%). Sedimentibacter was absent in R52SS but detected in the other samples, with a slightly higher level in digesters receiving straw (3.0–6.9%). For the class Bacilli, a higher relative abundance was seen in the manure samples (23.5–36.2%) than in all digester samples (2.3–8.6%, Fig. 3). The orders Lactobacillales and Turicibacterales represented the major fraction of sequences within this class in all samples (Fig. 4). Lactobacillales had a higher relative abundance in manure samples compared with digester samples.


Characterization of microbial community structure during continuous anaerobic digestion of straw and cow manure.

Sun L, Pope PB, Eijsink VG, Schnürer A - Microb Biotechnol (2015)

Relative abundance of bacterial 16S rRNA gene at the order level in manure (S1 and S2 correspond to duplicate samples) and in laboratory-scale digesters processing: (1) manure as sole substrate (RM); (2) stream-exploded straw and manure, operating constantly at 37°C (RTcSS); and (3) steam-exploded straw and manure, operating temperatures 37°C, 44°C and 52°C (R37SS, R44SS and R52SS), where R1 and R2 represent parallel digesters.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4554469&req=5

fig04: Relative abundance of bacterial 16S rRNA gene at the order level in manure (S1 and S2 correspond to duplicate samples) and in laboratory-scale digesters processing: (1) manure as sole substrate (RM); (2) stream-exploded straw and manure, operating constantly at 37°C (RTcSS); and (3) steam-exploded straw and manure, operating temperatures 37°C, 44°C and 52°C (R37SS, R44SS and R52SS), where R1 and R2 represent parallel digesters.
Mentions: Firmicutes was observed in all digesters as well as in the manure samples, with the highest relative abundance in the digesters operated at 52°C (97.6–97.8%), followed by the digesters operated at 44°C (76.5–82.5%). In the remaining samples, the relative abundance of sequences belonging to this phylum was 56.8–74.1% (manure), 45.9–50.4% (RM, digester operated with manure alone) and 32.6–49.5% (R37SS and RTcSS, Fig. S3). Within the Firmicutes, sequences belonging to the classes Bacilli and Clostridia dominated (Fig. 3). The class Clostridia was represented in similar levels in the digesters operated at 37°C (26.8–41.9%) and in the manure samples (30.4–34.9%). However, in line with the increase in operating temperature, the relative abundance of sequences within this class increased and was 66.8–77.2% and 92.4–93.8% in R44SS and R52SS respectively. Within the class Clostridia, sequences belonging to the order Clostridiales and to the uncultured order MBA08 were found to be dominant (Fig. 4). Clostridiales was present in manure (29.4–33.7%) and all digesters (10.1–38.8) with the lowest relative abundance at 52°C (10.1–15.0%), while MBA08 was not detected in manure but in all digesters (1.9–74.4%) with the highest relative abundance at 52°C (67.1–74.4%). Within the order Clostridiales, the families Peptostreptococcaceae (5.4–18.1%) and Clostridiaceae (2.2–11.1%) were identified as the major groups. In addition, Lachnospiraceae, Ruminococcaceae, Syntrophomonadaceae, Peptococcaceae, Catabacteriaceae, Eubacteriaceae and Veillonellaceae were present at lower levels (data not shown). Within the Clostridiaceae, sequences belonging to the genera Clostridium and Sedimentibacter dominated (Fig. S2). Clostridium was detected in all sampling points (0.7–4.4%). Sedimentibacter was absent in R52SS but detected in the other samples, with a slightly higher level in digesters receiving straw (3.0–6.9%). For the class Bacilli, a higher relative abundance was seen in the manure samples (23.5–36.2%) than in all digester samples (2.3–8.6%, Fig. 3). The orders Lactobacillales and Turicibacterales represented the major fraction of sequences within this class in all samples (Fig. 4). Lactobacillales had a higher relative abundance in manure samples compared with digester samples.

Bottom Line: Compared with manure itself, digestion of manure resulted in a higher abundance of an uncultured class WWE1 and lower abundance of Bacilli.Adding straw to the digesters increased the level of Bacteroidia, while increasing the operating temperature decreased the level of this class and instead increased the relative abundance of an uncultured genus affiliated to order MBA08 (Clostridia).A considerable fraction of bacterial sequences could not be allocated to genus level, indicating that novel phylotypes are resident in these communities.

View Article: PubMed Central - PubMed

Affiliation: Department of Microbiology, Swedish University of Agricultural Science, Uppsala BioCenter, P.O. Box 7025, SE-750 07, Uppsala, Sweden.

No MeSH data available.