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Characterization of wheat straw-degrading anaerobic alkali-tolerant mixed cultures from soda lake sediments by molecular and cultivation techniques.

Porsch K, Wirth B, Tóth EM, Schattenberg F, Nikolausz M - Microb Biotechnol (2015)

Bottom Line: Methanothermobacter and Methanosarcina genera and the order Methanomicrobiales were predominant methanogenes in the obtained cultures.Additionally, single cellulolytic microorganisms were isolated from enrichment cultures and identified as members of the alkaliphilic or alkalitolerant genera.The results show that anaerobic alkaline habitats harbor diverse microbial communities, which can degrade lignocellulose effectively and are therefore a potential resource for improving anaerobic digestion.

View Article: PubMed Central - PubMed

Affiliation: Department of Bioenergy, Helmholtz Centre for Environmental Research - UFZ, Leipzig, Germany.

No MeSH data available.


Related in: MedlinePlus

Relative T-RF abundance of the bacterial 16S rRNA genes digested with the restriction enzyme MspI of the sediment samples (Sed), first enrichment culture (En) and sixth or fifth transfer (6.T or 5.T) of the mixed cultures obtained from the Lake Szarvas (S37°C and S55°C) and Lake Velencei (V37°C) incubated at 37°C or 55°C. Terminal restriction fragments with an abundance < 2% are not shown. Each color represents one T-RF and major T-RFs of the same length are connected by lines. Microorganisms producing certain T-RFs were identified by cloning and sequencing.
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fig03: Relative T-RF abundance of the bacterial 16S rRNA genes digested with the restriction enzyme MspI of the sediment samples (Sed), first enrichment culture (En) and sixth or fifth transfer (6.T or 5.T) of the mixed cultures obtained from the Lake Szarvas (S37°C and S55°C) and Lake Velencei (V37°C) incubated at 37°C or 55°C. Terminal restriction fragments with an abundance < 2% are not shown. Each color represents one T-RF and major T-RFs of the same length are connected by lines. Microorganisms producing certain T-RFs were identified by cloning and sequencing.

Mentions: In order to gain more details about the taxonomy of the community members, clone libraries have been established from the sixth transfer of V37°C and S37°C and from the fifth transfer of S55°C. After screening of the PCR amplicons from the clones by T-RFLP, members of the predominant T-RF groups have been partially sequenced. In this way, we linked taxonomic affiliation to the most dominant T-RF peaks (Fig. 3). The two communities obtained from different lakes under the same condition (incubation temperature 37°C) were completely different at species level (using less than 97% similarity value as definition of distinction). However, looking at the affiliation of the sequences at higher taxonomical ranks, the two cultures can be considered as similar. Both enrichment cultures can be described by the predominance of bacteria affiliated to the orders Sphingobacteriales, Clostridiales and the family Spirochaetaceae.


Characterization of wheat straw-degrading anaerobic alkali-tolerant mixed cultures from soda lake sediments by molecular and cultivation techniques.

Porsch K, Wirth B, Tóth EM, Schattenberg F, Nikolausz M - Microb Biotechnol (2015)

Relative T-RF abundance of the bacterial 16S rRNA genes digested with the restriction enzyme MspI of the sediment samples (Sed), first enrichment culture (En) and sixth or fifth transfer (6.T or 5.T) of the mixed cultures obtained from the Lake Szarvas (S37°C and S55°C) and Lake Velencei (V37°C) incubated at 37°C or 55°C. Terminal restriction fragments with an abundance < 2% are not shown. Each color represents one T-RF and major T-RFs of the same length are connected by lines. Microorganisms producing certain T-RFs were identified by cloning and sequencing.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4554468&req=5

fig03: Relative T-RF abundance of the bacterial 16S rRNA genes digested with the restriction enzyme MspI of the sediment samples (Sed), first enrichment culture (En) and sixth or fifth transfer (6.T or 5.T) of the mixed cultures obtained from the Lake Szarvas (S37°C and S55°C) and Lake Velencei (V37°C) incubated at 37°C or 55°C. Terminal restriction fragments with an abundance < 2% are not shown. Each color represents one T-RF and major T-RFs of the same length are connected by lines. Microorganisms producing certain T-RFs were identified by cloning and sequencing.
Mentions: In order to gain more details about the taxonomy of the community members, clone libraries have been established from the sixth transfer of V37°C and S37°C and from the fifth transfer of S55°C. After screening of the PCR amplicons from the clones by T-RFLP, members of the predominant T-RF groups have been partially sequenced. In this way, we linked taxonomic affiliation to the most dominant T-RF peaks (Fig. 3). The two communities obtained from different lakes under the same condition (incubation temperature 37°C) were completely different at species level (using less than 97% similarity value as definition of distinction). However, looking at the affiliation of the sequences at higher taxonomical ranks, the two cultures can be considered as similar. Both enrichment cultures can be described by the predominance of bacteria affiliated to the orders Sphingobacteriales, Clostridiales and the family Spirochaetaceae.

Bottom Line: Methanothermobacter and Methanosarcina genera and the order Methanomicrobiales were predominant methanogenes in the obtained cultures.Additionally, single cellulolytic microorganisms were isolated from enrichment cultures and identified as members of the alkaliphilic or alkalitolerant genera.The results show that anaerobic alkaline habitats harbor diverse microbial communities, which can degrade lignocellulose effectively and are therefore a potential resource for improving anaerobic digestion.

View Article: PubMed Central - PubMed

Affiliation: Department of Bioenergy, Helmholtz Centre for Environmental Research - UFZ, Leipzig, Germany.

No MeSH data available.


Related in: MedlinePlus