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Inoculum selection influences the biochemical methane potential of agro-industrial substrates.

De Vrieze J, Raport L, Willems B, Verbrugge S, Volcke E, Meers E, Angenent LT, Boon N - Microb Biotechnol (2015)

Bottom Line: A significant effect of the selected inoculum on the BMP result was observed for two out of four substrates.This inoculum effect could be attributed to the abundance of methanogens and a potential inhibiting effect in the inoculum itself, demonstrating the importance of inoculum selection for BMP testing.We recommend the application of granular sludge as an inoculum, because of its higher methanogenic abundance and activity, and protection from bulk solutions, compared with other inocula.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Microbial Ecology and Technology (LabMET), Ghent University, Coupure Links 653, Gent, B-9000, Belgium.

No MeSH data available.


Methane yield curves of the molasses (), bio-refinery waste (), liquid manure (), and A-sludge () substrates for the (A) OBW, (B) MAN, (C) BREW and (D) ENG inocula. Error bars show standard deviations.
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fig02: Methane yield curves of the molasses (), bio-refinery waste (), liquid manure (), and A-sludge () substrates for the (A) OBW, (B) MAN, (C) BREW and (D) ENG inocula. Error bars show standard deviations.

Mentions: The OBW and MAN inocula showed similar final methane yield results for all four substrates, although methane yield was always slightly higher for the OBW inoculum (Fig. 1A and B). In accordance, a similar methane production profile in function of time was observed for both inocula (Fig. 2A and B). The BREW inoculum, with the exception of the A-sludge substrate, produced the highest methane yield values for each substrate. Moreover, methane production showed very high values, especially for the molasses and bio-refinery waste substrates, during the first few days of the BMP experiment, indicating high methanogenic activity (Fig. 2C). These three inocula (OBW, MAN and BREW) reached a clear plateau phase after 35 days. The ENG inoculum showed a difficult start-up for each sample, indicating low methanogenic activity, and the plateau phase, apparently, was not yet obtained after 35 days (Fig. 2D). The methane yield of the negative control treatments is presented in the Supporting Information (SI) (Fig. S1).


Inoculum selection influences the biochemical methane potential of agro-industrial substrates.

De Vrieze J, Raport L, Willems B, Verbrugge S, Volcke E, Meers E, Angenent LT, Boon N - Microb Biotechnol (2015)

Methane yield curves of the molasses (), bio-refinery waste (), liquid manure (), and A-sludge () substrates for the (A) OBW, (B) MAN, (C) BREW and (D) ENG inocula. Error bars show standard deviations.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4554466&req=5

fig02: Methane yield curves of the molasses (), bio-refinery waste (), liquid manure (), and A-sludge () substrates for the (A) OBW, (B) MAN, (C) BREW and (D) ENG inocula. Error bars show standard deviations.
Mentions: The OBW and MAN inocula showed similar final methane yield results for all four substrates, although methane yield was always slightly higher for the OBW inoculum (Fig. 1A and B). In accordance, a similar methane production profile in function of time was observed for both inocula (Fig. 2A and B). The BREW inoculum, with the exception of the A-sludge substrate, produced the highest methane yield values for each substrate. Moreover, methane production showed very high values, especially for the molasses and bio-refinery waste substrates, during the first few days of the BMP experiment, indicating high methanogenic activity (Fig. 2C). These three inocula (OBW, MAN and BREW) reached a clear plateau phase after 35 days. The ENG inoculum showed a difficult start-up for each sample, indicating low methanogenic activity, and the plateau phase, apparently, was not yet obtained after 35 days (Fig. 2D). The methane yield of the negative control treatments is presented in the Supporting Information (SI) (Fig. S1).

Bottom Line: A significant effect of the selected inoculum on the BMP result was observed for two out of four substrates.This inoculum effect could be attributed to the abundance of methanogens and a potential inhibiting effect in the inoculum itself, demonstrating the importance of inoculum selection for BMP testing.We recommend the application of granular sludge as an inoculum, because of its higher methanogenic abundance and activity, and protection from bulk solutions, compared with other inocula.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Microbial Ecology and Technology (LabMET), Ghent University, Coupure Links 653, Gent, B-9000, Belgium.

No MeSH data available.