Limits...
Inoculum selection influences the biochemical methane potential of agro-industrial substrates.

De Vrieze J, Raport L, Willems B, Verbrugge S, Volcke E, Meers E, Angenent LT, Boon N - Microb Biotechnol (2015)

Bottom Line: A significant effect of the selected inoculum on the BMP result was observed for two out of four substrates.This inoculum effect could be attributed to the abundance of methanogens and a potential inhibiting effect in the inoculum itself, demonstrating the importance of inoculum selection for BMP testing.We recommend the application of granular sludge as an inoculum, because of its higher methanogenic abundance and activity, and protection from bulk solutions, compared with other inocula.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Microbial Ecology and Technology (LabMET), Ghent University, Coupure Links 653, Gent, B-9000, Belgium.

No MeSH data available.


Ultimate methane yields (ml CH4 g−1 VS) of the (A) molasses, (B) bio-refinery waste, (C) liquid manure, and (D) A-sludge substrates. Error bars show standard deviations, and different letters (A, B and C) indicate significant differences according to ANOVA and subsequent multiple comparison by a Tukey hsd test at the 5% significance level.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4554466&req=5

fig01: Ultimate methane yields (ml CH4 g−1 VS) of the (A) molasses, (B) bio-refinery waste, (C) liquid manure, and (D) A-sludge substrates. Error bars show standard deviations, and different letters (A, B and C) indicate significant differences according to ANOVA and subsequent multiple comparison by a Tukey hsd test at the 5% significance level.

Mentions: Incubation with different inocula resulted in similar BMP values for molasses, because no significant differences were observed between the different inocula (Fig. 1A). An average value of 363 ± 18 ml CH4 g−1 volatile solids (VS) was observed, which related to a COD conversion efficiency of 79.7 ± 4.0%. Similar to molasses, no significant differences were observed between the different inocula for the BMP determination of the bio-refinery waste substrate (Fig. 1B). An average methane yield value of 213 ± 36 ml CH4 g−1 VS and COD conversion efficiency of 33.1 ± 5.6% were obtained. The BMP test with liquid pig manure as substrate showed a significant difference in BMP results between the MAN and BREW inoculum (Fig. 1C), with a value of 137 ± 28 and 330 ± 38 ml CH4 g−1 VS, and a COD conversion efficiency of 20.7 ± 4.3 and 49.7 ± 5.7% for the MAN and BREW inocula respectively. The determination of the BMP of A-sludge resulted in a significant higher BMP value for the ENG inoculum (Fig. 1D), compared with the OBW and MAN inocula, with methane yield values of 455 ± 26, 347 ± 14 and 304 ± 45 ml CH4 g−1 VS, and COD conversion efficiencies of 75.2 ± 4.3, 58.6 ± 2.4 and 51.2 ± 7.6% for the ENG, OBW and MAN inocula respectively. The BREW inoculum (methane yield of 406 ml CH4 g−1 VS and COD conversion efficiency of 68.5%) also reached a significant higher BMP result than the MAN inoculum for the A-sludge.


Inoculum selection influences the biochemical methane potential of agro-industrial substrates.

De Vrieze J, Raport L, Willems B, Verbrugge S, Volcke E, Meers E, Angenent LT, Boon N - Microb Biotechnol (2015)

Ultimate methane yields (ml CH4 g−1 VS) of the (A) molasses, (B) bio-refinery waste, (C) liquid manure, and (D) A-sludge substrates. Error bars show standard deviations, and different letters (A, B and C) indicate significant differences according to ANOVA and subsequent multiple comparison by a Tukey hsd test at the 5% significance level.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4554466&req=5

fig01: Ultimate methane yields (ml CH4 g−1 VS) of the (A) molasses, (B) bio-refinery waste, (C) liquid manure, and (D) A-sludge substrates. Error bars show standard deviations, and different letters (A, B and C) indicate significant differences according to ANOVA and subsequent multiple comparison by a Tukey hsd test at the 5% significance level.
Mentions: Incubation with different inocula resulted in similar BMP values for molasses, because no significant differences were observed between the different inocula (Fig. 1A). An average value of 363 ± 18 ml CH4 g−1 volatile solids (VS) was observed, which related to a COD conversion efficiency of 79.7 ± 4.0%. Similar to molasses, no significant differences were observed between the different inocula for the BMP determination of the bio-refinery waste substrate (Fig. 1B). An average methane yield value of 213 ± 36 ml CH4 g−1 VS and COD conversion efficiency of 33.1 ± 5.6% were obtained. The BMP test with liquid pig manure as substrate showed a significant difference in BMP results between the MAN and BREW inoculum (Fig. 1C), with a value of 137 ± 28 and 330 ± 38 ml CH4 g−1 VS, and a COD conversion efficiency of 20.7 ± 4.3 and 49.7 ± 5.7% for the MAN and BREW inocula respectively. The determination of the BMP of A-sludge resulted in a significant higher BMP value for the ENG inoculum (Fig. 1D), compared with the OBW and MAN inocula, with methane yield values of 455 ± 26, 347 ± 14 and 304 ± 45 ml CH4 g−1 VS, and COD conversion efficiencies of 75.2 ± 4.3, 58.6 ± 2.4 and 51.2 ± 7.6% for the ENG, OBW and MAN inocula respectively. The BREW inoculum (methane yield of 406 ml CH4 g−1 VS and COD conversion efficiency of 68.5%) also reached a significant higher BMP result than the MAN inoculum for the A-sludge.

Bottom Line: A significant effect of the selected inoculum on the BMP result was observed for two out of four substrates.This inoculum effect could be attributed to the abundance of methanogens and a potential inhibiting effect in the inoculum itself, demonstrating the importance of inoculum selection for BMP testing.We recommend the application of granular sludge as an inoculum, because of its higher methanogenic abundance and activity, and protection from bulk solutions, compared with other inocula.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Microbial Ecology and Technology (LabMET), Ghent University, Coupure Links 653, Gent, B-9000, Belgium.

No MeSH data available.