Limits...
Protective role of bacillithiol in superoxide stress and Fe-S metabolism in Bacillus subtilis.

Fang Z, Dos Santos PC - Microbiologyopen (2015)

Bottom Line: Interestingly, Fe-S cluster containing isopropylmalate isomerase (LeuCD) and glutamate synthase (GOGAT) showed decreased activities in BSH strain.Deficiency of BSH also resulted in decreased levels of intracellular Fe accompanied by increased levels of manganese and altered expression levels of Fe-S cluster biosynthetic SUF components.Together, this study is the first to establish a link between BSH and Fe-S metabolism in B. subtilis.

View Article: PubMed Central - PubMed

Affiliation: Department of Chemistry, Wake Forest University, Winston-Salem, North Carolina, 27016.

No MeSH data available.


Related in: MedlinePlus

Growth curves of wild-type Bacillus subtilis (left panel) and ΔbshA strain (right panel) in MM with 0.05% casamino acid in presence of various stress challenges. (A) Cultures contained 0 μmol/L (square), 100 μmol/L (circle), and 300 μmol/L (triangle) of CuCl; (B) Cultures contained 0 μmol/L (square), 20 μmol/L (circle), and 50 μmol/L (triangle) of CdCl2. (C) Cultures contained 0 μmol/L (square), 100 μmol/L (circle), and 200 μmol/L (triangle) of 2,2′-dypridyl. (D) Cultures contained 0 μmol/L (square), 100 μmol/L (circle), and 250 μmol/L (triangle) of paraquat. The curves shown are representatives of at least three independent experiments.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4554457&req=5

fig03: Growth curves of wild-type Bacillus subtilis (left panel) and ΔbshA strain (right panel) in MM with 0.05% casamino acid in presence of various stress challenges. (A) Cultures contained 0 μmol/L (square), 100 μmol/L (circle), and 300 μmol/L (triangle) of CuCl; (B) Cultures contained 0 μmol/L (square), 20 μmol/L (circle), and 50 μmol/L (triangle) of CdCl2. (C) Cultures contained 0 μmol/L (square), 100 μmol/L (circle), and 200 μmol/L (triangle) of 2,2′-dypridyl. (D) Cultures contained 0 μmol/L (square), 100 μmol/L (circle), and 250 μmol/L (triangle) of paraquat. The curves shown are representatives of at least three independent experiments.

Mentions: The effects of oxidative stress on the B. subtilis ΔbshA strain have been previously investigated using zone inhibition assays demonstrating that both wild-type and BSH− strain had the same sensitivity to H2O2 (Gaballa et al. 2010). Herein, the role of BSH against a wide range of stresses was investigated in MM supplemented with casamino acids. This growth medium not only ensured similar growth rates for both strains under no stress condition (Figs.1C, 3), but also eliminated the interference from potential protective biomolecules present in rich medium. Initial screens involved growth assays in presence of 0–300 μmol/L H2O2 (data not shown), which showed no differences between wild-type and ΔbshA strains, in agreement with a previous report (Gaballa et al. 2010). Given the involvement of GSH in metal homeostasis, Fe–S biogenesis, and bacterial response to environmental stressors, we investigated the growth profiles of both B. subtilis wild-type and bshA deletion strains under conditions known to destabilize metal homeostasis and Fe–S metabolism such as copper (Cu(I)), cadmium (Cd(II)), as well Fe starvation conditions.


Protective role of bacillithiol in superoxide stress and Fe-S metabolism in Bacillus subtilis.

Fang Z, Dos Santos PC - Microbiologyopen (2015)

Growth curves of wild-type Bacillus subtilis (left panel) and ΔbshA strain (right panel) in MM with 0.05% casamino acid in presence of various stress challenges. (A) Cultures contained 0 μmol/L (square), 100 μmol/L (circle), and 300 μmol/L (triangle) of CuCl; (B) Cultures contained 0 μmol/L (square), 20 μmol/L (circle), and 50 μmol/L (triangle) of CdCl2. (C) Cultures contained 0 μmol/L (square), 100 μmol/L (circle), and 200 μmol/L (triangle) of 2,2′-dypridyl. (D) Cultures contained 0 μmol/L (square), 100 μmol/L (circle), and 250 μmol/L (triangle) of paraquat. The curves shown are representatives of at least three independent experiments.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4554457&req=5

fig03: Growth curves of wild-type Bacillus subtilis (left panel) and ΔbshA strain (right panel) in MM with 0.05% casamino acid in presence of various stress challenges. (A) Cultures contained 0 μmol/L (square), 100 μmol/L (circle), and 300 μmol/L (triangle) of CuCl; (B) Cultures contained 0 μmol/L (square), 20 μmol/L (circle), and 50 μmol/L (triangle) of CdCl2. (C) Cultures contained 0 μmol/L (square), 100 μmol/L (circle), and 200 μmol/L (triangle) of 2,2′-dypridyl. (D) Cultures contained 0 μmol/L (square), 100 μmol/L (circle), and 250 μmol/L (triangle) of paraquat. The curves shown are representatives of at least three independent experiments.
Mentions: The effects of oxidative stress on the B. subtilis ΔbshA strain have been previously investigated using zone inhibition assays demonstrating that both wild-type and BSH− strain had the same sensitivity to H2O2 (Gaballa et al. 2010). Herein, the role of BSH against a wide range of stresses was investigated in MM supplemented with casamino acids. This growth medium not only ensured similar growth rates for both strains under no stress condition (Figs.1C, 3), but also eliminated the interference from potential protective biomolecules present in rich medium. Initial screens involved growth assays in presence of 0–300 μmol/L H2O2 (data not shown), which showed no differences between wild-type and ΔbshA strains, in agreement with a previous report (Gaballa et al. 2010). Given the involvement of GSH in metal homeostasis, Fe–S biogenesis, and bacterial response to environmental stressors, we investigated the growth profiles of both B. subtilis wild-type and bshA deletion strains under conditions known to destabilize metal homeostasis and Fe–S metabolism such as copper (Cu(I)), cadmium (Cd(II)), as well Fe starvation conditions.

Bottom Line: Interestingly, Fe-S cluster containing isopropylmalate isomerase (LeuCD) and glutamate synthase (GOGAT) showed decreased activities in BSH strain.Deficiency of BSH also resulted in decreased levels of intracellular Fe accompanied by increased levels of manganese and altered expression levels of Fe-S cluster biosynthetic SUF components.Together, this study is the first to establish a link between BSH and Fe-S metabolism in B. subtilis.

View Article: PubMed Central - PubMed

Affiliation: Department of Chemistry, Wake Forest University, Winston-Salem, North Carolina, 27016.

No MeSH data available.


Related in: MedlinePlus