Limits...
A factor converting viable but nonculturable Vibrio cholerae to a culturable state in eukaryotic cells is a human catalase.

Senoh M, Hamabata T, Takeda Y - Microbiologyopen (2015)

Bottom Line: Homogeneity of the purified FCVC was demonstrated by SDS-PAGE.Nano-LC MS/MS analysis showed that the purified FCVC was a human catalase.An experiment of RNAi knockdown of catalase mRNA from HT-29 cells and treatment of the purified FCVC with a catalase inhibitor, 3-amino-1,2,4-triazole confirmed that the FCVC was a catalase.

View Article: PubMed Central - PubMed

Affiliation: Collaborative Research Center of Okayama University for Infectious Diseases in India, Okayama University, Kolkata, India.

No MeSH data available.


Related in: MedlinePlus

Converting activity of several fractions of HT-29 cells. HT-29 cell extracts were fractionated, and the converting activity of each fraction was measured as described in the text. The converting activity was expressed as a percentage of the total activity in the cell extract. Bars represent means ± SD of three determinations.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4554454&req=5

fig01: Converting activity of several fractions of HT-29 cells. HT-29 cell extracts were fractionated, and the converting activity of each fraction was measured as described in the text. The converting activity was expressed as a percentage of the total activity in the cell extract. Bars represent means ± SD of three determinations.

Mentions: To examine whether FCVC has catalase activity, we fractionated the cellular proteins of HT-29 cells and measured their VBNC-converting activity. As shown in Figure1, the converting activity was localized mostly in the membrane and organelle fraction and slightly in the cytosolic fraction, with no activity in the nuclear and cytoskeleton fractions. These results are consistent with the fact that catalase is located in peroxisomes within cells (De Duve and Baudhuin 1966).


A factor converting viable but nonculturable Vibrio cholerae to a culturable state in eukaryotic cells is a human catalase.

Senoh M, Hamabata T, Takeda Y - Microbiologyopen (2015)

Converting activity of several fractions of HT-29 cells. HT-29 cell extracts were fractionated, and the converting activity of each fraction was measured as described in the text. The converting activity was expressed as a percentage of the total activity in the cell extract. Bars represent means ± SD of three determinations.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4554454&req=5

fig01: Converting activity of several fractions of HT-29 cells. HT-29 cell extracts were fractionated, and the converting activity of each fraction was measured as described in the text. The converting activity was expressed as a percentage of the total activity in the cell extract. Bars represent means ± SD of three determinations.
Mentions: To examine whether FCVC has catalase activity, we fractionated the cellular proteins of HT-29 cells and measured their VBNC-converting activity. As shown in Figure1, the converting activity was localized mostly in the membrane and organelle fraction and slightly in the cytosolic fraction, with no activity in the nuclear and cytoskeleton fractions. These results are consistent with the fact that catalase is located in peroxisomes within cells (De Duve and Baudhuin 1966).

Bottom Line: Homogeneity of the purified FCVC was demonstrated by SDS-PAGE.Nano-LC MS/MS analysis showed that the purified FCVC was a human catalase.An experiment of RNAi knockdown of catalase mRNA from HT-29 cells and treatment of the purified FCVC with a catalase inhibitor, 3-amino-1,2,4-triazole confirmed that the FCVC was a catalase.

View Article: PubMed Central - PubMed

Affiliation: Collaborative Research Center of Okayama University for Infectious Diseases in India, Okayama University, Kolkata, India.

No MeSH data available.


Related in: MedlinePlus