Limits...
Comparative genomics of Pseudomonas syringae pv. syringae strains B301D and HS191 and insights into intrapathovar traits associated with plant pathogenesis.

Ravindran A, Jalan N, Yuan JS, Wang N, Gross DC - Microbiologyopen (2015)

Bottom Line: Differences are observed in the type III effector composition for the three strains that likely influences host range.The HS191 genome had the largest number at 25 of effector genes, and seven effector genes are specific to this monocot strain.Toxin production is another major trait associated with virulence of P. syringae pv. syringae, and HS191 is distinguished by genes for production of syringopeptin SP25 and mangotoxin.

View Article: PubMed Central - PubMed

Affiliation: Department of Plant Pathology and Microbiology, Texas A&M University, College Station, Texas, 77843-2132.

No MeSH data available.


Related in: MedlinePlus

Venn diagram comparing the unique and shared genes between the genomes of B728a (top), B301D (left), and HS191 (right) using BLASTp. The numbers below the strain names identify the total number of protein coding genes within each genome. The strain HS191 genome has 645 unique genes, including 61 unique genes on plasmid pCG131. Genes that are conserved among all three strains are shown in the center of the diagram as the pangenome.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4554452&req=5

fig04: Venn diagram comparing the unique and shared genes between the genomes of B728a (top), B301D (left), and HS191 (right) using BLASTp. The numbers below the strain names identify the total number of protein coding genes within each genome. The strain HS191 genome has 645 unique genes, including 61 unique genes on plasmid pCG131. Genes that are conserved among all three strains are shown in the center of the diagram as the pangenome.

Mentions: Horizontal gene transfer (HGT) plays a significant role in the diversification of lineages in P. syringae especially in regard to the evolution of defined pathovars (Nowell et al. 2014). BRIG analysis of the B728a, B301D, and HS191 genomes suggests that most of the unique gene regions (Fig.3) are associated with HGT as they frequently carried transposons, phage elements, and IS elements. The genome regions identified by BRIG analysis are largely composed of unique genes, ranging from 45% to 60% of the total number of unique genes in a given strain as depicted by a Venn diagram (Fig.4). In addition, BRIG analysis also was useful in identifying shared gene regions present in two strains, but absent in the other strain.


Comparative genomics of Pseudomonas syringae pv. syringae strains B301D and HS191 and insights into intrapathovar traits associated with plant pathogenesis.

Ravindran A, Jalan N, Yuan JS, Wang N, Gross DC - Microbiologyopen (2015)

Venn diagram comparing the unique and shared genes between the genomes of B728a (top), B301D (left), and HS191 (right) using BLASTp. The numbers below the strain names identify the total number of protein coding genes within each genome. The strain HS191 genome has 645 unique genes, including 61 unique genes on plasmid pCG131. Genes that are conserved among all three strains are shown in the center of the diagram as the pangenome.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4554452&req=5

fig04: Venn diagram comparing the unique and shared genes between the genomes of B728a (top), B301D (left), and HS191 (right) using BLASTp. The numbers below the strain names identify the total number of protein coding genes within each genome. The strain HS191 genome has 645 unique genes, including 61 unique genes on plasmid pCG131. Genes that are conserved among all three strains are shown in the center of the diagram as the pangenome.
Mentions: Horizontal gene transfer (HGT) plays a significant role in the diversification of lineages in P. syringae especially in regard to the evolution of defined pathovars (Nowell et al. 2014). BRIG analysis of the B728a, B301D, and HS191 genomes suggests that most of the unique gene regions (Fig.3) are associated with HGT as they frequently carried transposons, phage elements, and IS elements. The genome regions identified by BRIG analysis are largely composed of unique genes, ranging from 45% to 60% of the total number of unique genes in a given strain as depicted by a Venn diagram (Fig.4). In addition, BRIG analysis also was useful in identifying shared gene regions present in two strains, but absent in the other strain.

Bottom Line: Differences are observed in the type III effector composition for the three strains that likely influences host range.The HS191 genome had the largest number at 25 of effector genes, and seven effector genes are specific to this monocot strain.Toxin production is another major trait associated with virulence of P. syringae pv. syringae, and HS191 is distinguished by genes for production of syringopeptin SP25 and mangotoxin.

View Article: PubMed Central - PubMed

Affiliation: Department of Plant Pathology and Microbiology, Texas A&M University, College Station, Texas, 77843-2132.

No MeSH data available.


Related in: MedlinePlus