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Lactobacillus fermentum AGR1487 cell surface structures and supernatant increase paracellular permeability through different pathways.

Sengupta R, Anderson RC, Altermann E, McNabb WC, Ganesh S, Armstrong KM, Moughan PJ, Roy NC - Microbiologyopen (2015)

Bottom Line: Lactobacillus fermentum is commonly found in food products, and some strains are known to have beneficial effects on human health.Both live and UV-inactivated AGR1487 decreased TEER across Caco-2 cells implicating the cell surfaces structures in the effect.These differences in barrier integrity results are likely due to the TEER and mannitol flux assays measuring different characteristics of the epithelial barrier, and therefore imply that there are multiple mechanisms involved in the effect of AGR1487 on barrier integrity.

View Article: PubMed Central - PubMed

Affiliation: Food Nutrition & Health Team, Food & Bio-based Products Group, AgResearch Grasslands, Palmerston North, 4442, New Zealand.

No MeSH data available.


Related in: MedlinePlus

Effect of live and UV-killed AGR1485 and AGR1487 on (A) TEER and (B) mannitol flux across Caco-2 cell monolayers over time. The plotted values are the means of 12 replicates (four replicates per run, three independent runs) and the error bars show the SEM. For the TEER data, the least significant difference levels at 5% probability (5% LSD) is given. For the mannitol flux data, the *indicate P < 0.05 compared to the control.
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fig06: Effect of live and UV-killed AGR1485 and AGR1487 on (A) TEER and (B) mannitol flux across Caco-2 cell monolayers over time. The plotted values are the means of 12 replicates (four replicates per run, three independent runs) and the error bars show the SEM. For the TEER data, the least significant difference levels at 5% probability (5% LSD) is given. For the mannitol flux data, the *indicate P < 0.05 compared to the control.

Mentions: Both live and UV-inactivated AGR1485 did not significantly alter TEER compared to control media; whereas both live and UV-inactivated AGR1487 caused a decrease in TEER (Fig.6A). Pairwise comparison showed no significant difference between the effects of live AGR1487 and UV-inactivated AGR1487 at any time point. For AGR1485, in agreement with the TEER data, the mannitol flux results showed that compared to control medium, neither live nor UV-inactivated AGR1485 changed the rate at which mannitol crossed the Caco-2 cell layers (Fig.6B). As expected, live AGR1487 increased the passage of mannitol across the cell layer compared to control media; 17% versus 5.5% mannitol passed across the cell layer after 12 h (P < 0.05). Surprisingly, UV-inactivated AGR1487 did not cause an increase in mannitol flux compared to control medium, and pairwise comparison showed that there were significant differences between the effects of live AGR1487 and UV-killed AGR1487 on the percentage of mannitol that had crossed the cell layer at 10 and 12 h.


Lactobacillus fermentum AGR1487 cell surface structures and supernatant increase paracellular permeability through different pathways.

Sengupta R, Anderson RC, Altermann E, McNabb WC, Ganesh S, Armstrong KM, Moughan PJ, Roy NC - Microbiologyopen (2015)

Effect of live and UV-killed AGR1485 and AGR1487 on (A) TEER and (B) mannitol flux across Caco-2 cell monolayers over time. The plotted values are the means of 12 replicates (four replicates per run, three independent runs) and the error bars show the SEM. For the TEER data, the least significant difference levels at 5% probability (5% LSD) is given. For the mannitol flux data, the *indicate P < 0.05 compared to the control.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4554451&req=5

fig06: Effect of live and UV-killed AGR1485 and AGR1487 on (A) TEER and (B) mannitol flux across Caco-2 cell monolayers over time. The plotted values are the means of 12 replicates (four replicates per run, three independent runs) and the error bars show the SEM. For the TEER data, the least significant difference levels at 5% probability (5% LSD) is given. For the mannitol flux data, the *indicate P < 0.05 compared to the control.
Mentions: Both live and UV-inactivated AGR1485 did not significantly alter TEER compared to control media; whereas both live and UV-inactivated AGR1487 caused a decrease in TEER (Fig.6A). Pairwise comparison showed no significant difference between the effects of live AGR1487 and UV-inactivated AGR1487 at any time point. For AGR1485, in agreement with the TEER data, the mannitol flux results showed that compared to control medium, neither live nor UV-inactivated AGR1485 changed the rate at which mannitol crossed the Caco-2 cell layers (Fig.6B). As expected, live AGR1487 increased the passage of mannitol across the cell layer compared to control media; 17% versus 5.5% mannitol passed across the cell layer after 12 h (P < 0.05). Surprisingly, UV-inactivated AGR1487 did not cause an increase in mannitol flux compared to control medium, and pairwise comparison showed that there were significant differences between the effects of live AGR1487 and UV-killed AGR1487 on the percentage of mannitol that had crossed the cell layer at 10 and 12 h.

Bottom Line: Lactobacillus fermentum is commonly found in food products, and some strains are known to have beneficial effects on human health.Both live and UV-inactivated AGR1487 decreased TEER across Caco-2 cells implicating the cell surfaces structures in the effect.These differences in barrier integrity results are likely due to the TEER and mannitol flux assays measuring different characteristics of the epithelial barrier, and therefore imply that there are multiple mechanisms involved in the effect of AGR1487 on barrier integrity.

View Article: PubMed Central - PubMed

Affiliation: Food Nutrition & Health Team, Food & Bio-based Products Group, AgResearch Grasslands, Palmerston North, 4442, New Zealand.

No MeSH data available.


Related in: MedlinePlus