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Dopamine-functionalized InP/ZnS quantum dots as fluorescence probes for the detection of adenosine in microfluidic chip.

Ankireddy SR, Kim J - Int J Nanomedicine (2015)

Bottom Line: Microbeads are frequently used as solid supports for biomolecules such as proteins and nucleic acids in heterogeneous microfluidic assays.The photoluminescence (PL) intensity of the QDs-DA is quenched by Zn(2+) because of the strong coordination interactions.In the presence of adenosine, Zn(2+) cations preferentially bind to adenosine, and the PL intensity of the QDs-DA is recovered.

View Article: PubMed Central - PubMed

Affiliation: Department of chemical and Biological Engineering, Gachon University, Seongnam, Gyeonggi-Do, South Korea.

ABSTRACT
Microbeads are frequently used as solid supports for biomolecules such as proteins and nucleic acids in heterogeneous microfluidic assays. Chip-based, quantum dot (QD)-bead-biomolecule probes have been used for the detection of various types of DNA. In this study, we developed dopamine (DA)-functionalized InP/ZnS QDs (QDs-DA) as fluorescence probes for the detection of adenosine in microfluidic chips. The photoluminescence (PL) intensity of the QDs-DA is quenched by Zn(2+) because of the strong coordination interactions. In the presence of adenosine, Zn(2+) cations preferentially bind to adenosine, and the PL intensity of the QDs-DA is recovered. A polydimethylsiloxane-based microfluidic chip was fabricated, and adenosine detection was confirmed using QDs-DA probes.

No MeSH data available.


Photographs of QDs (A) before and (B) after surface modification with MPA under UV illumination.Abbreviations: QDs, quantum dots; MPA, mercaptopropionic acid; UV, ultraviolet.
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f4-ijn-10-121: Photographs of QDs (A) before and (B) after surface modification with MPA under UV illumination.Abbreviations: QDs, quantum dots; MPA, mercaptopropionic acid; UV, ultraviolet.

Mentions: Figure 4A and B, respectively, shows photographs of the QDs before and after surface modification with MPA under ultraviolet (UV) illumination. The InP/ZnS QDs are dispersed in a two-layered mixture of n-hexane (upper) and deionized water (lower). The figure shows that before the surface treatment, the QDs were dispersed in the organic phase, but after treatment with MPA, they were dispersed in the water phase since the MPA ligand was water soluble. These surface modified QDs were used for the detection of adenosine in presence of Zn2+.


Dopamine-functionalized InP/ZnS quantum dots as fluorescence probes for the detection of adenosine in microfluidic chip.

Ankireddy SR, Kim J - Int J Nanomedicine (2015)

Photographs of QDs (A) before and (B) after surface modification with MPA under UV illumination.Abbreviations: QDs, quantum dots; MPA, mercaptopropionic acid; UV, ultraviolet.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4554435&req=5

f4-ijn-10-121: Photographs of QDs (A) before and (B) after surface modification with MPA under UV illumination.Abbreviations: QDs, quantum dots; MPA, mercaptopropionic acid; UV, ultraviolet.
Mentions: Figure 4A and B, respectively, shows photographs of the QDs before and after surface modification with MPA under ultraviolet (UV) illumination. The InP/ZnS QDs are dispersed in a two-layered mixture of n-hexane (upper) and deionized water (lower). The figure shows that before the surface treatment, the QDs were dispersed in the organic phase, but after treatment with MPA, they were dispersed in the water phase since the MPA ligand was water soluble. These surface modified QDs were used for the detection of adenosine in presence of Zn2+.

Bottom Line: Microbeads are frequently used as solid supports for biomolecules such as proteins and nucleic acids in heterogeneous microfluidic assays.The photoluminescence (PL) intensity of the QDs-DA is quenched by Zn(2+) because of the strong coordination interactions.In the presence of adenosine, Zn(2+) cations preferentially bind to adenosine, and the PL intensity of the QDs-DA is recovered.

View Article: PubMed Central - PubMed

Affiliation: Department of chemical and Biological Engineering, Gachon University, Seongnam, Gyeonggi-Do, South Korea.

ABSTRACT
Microbeads are frequently used as solid supports for biomolecules such as proteins and nucleic acids in heterogeneous microfluidic assays. Chip-based, quantum dot (QD)-bead-biomolecule probes have been used for the detection of various types of DNA. In this study, we developed dopamine (DA)-functionalized InP/ZnS QDs (QDs-DA) as fluorescence probes for the detection of adenosine in microfluidic chips. The photoluminescence (PL) intensity of the QDs-DA is quenched by Zn(2+) because of the strong coordination interactions. In the presence of adenosine, Zn(2+) cations preferentially bind to adenosine, and the PL intensity of the QDs-DA is recovered. A polydimethylsiloxane-based microfluidic chip was fabricated, and adenosine detection was confirmed using QDs-DA probes.

No MeSH data available.