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Dopamine-functionalized InP/ZnS quantum dots as fluorescence probes for the detection of adenosine in microfluidic chip.

Ankireddy SR, Kim J - Int J Nanomedicine (2015)

Bottom Line: Microbeads are frequently used as solid supports for biomolecules such as proteins and nucleic acids in heterogeneous microfluidic assays.The photoluminescence (PL) intensity of the QDs-DA is quenched by Zn(2+) because of the strong coordination interactions.In the presence of adenosine, Zn(2+) cations preferentially bind to adenosine, and the PL intensity of the QDs-DA is recovered.

View Article: PubMed Central - PubMed

Affiliation: Department of chemical and Biological Engineering, Gachon University, Seongnam, Gyeonggi-Do, South Korea.

ABSTRACT
Microbeads are frequently used as solid supports for biomolecules such as proteins and nucleic acids in heterogeneous microfluidic assays. Chip-based, quantum dot (QD)-bead-biomolecule probes have been used for the detection of various types of DNA. In this study, we developed dopamine (DA)-functionalized InP/ZnS QDs (QDs-DA) as fluorescence probes for the detection of adenosine in microfluidic chips. The photoluminescence (PL) intensity of the QDs-DA is quenched by Zn(2+) because of the strong coordination interactions. In the presence of adenosine, Zn(2+) cations preferentially bind to adenosine, and the PL intensity of the QDs-DA is recovered. A polydimethylsiloxane-based microfluidic chip was fabricated, and adenosine detection was confirmed using QDs-DA probes.

No MeSH data available.


Related in: MedlinePlus

Photographs of (A) photomask for photoresist process for SU-8 molding, inset photo shows the amplified image of pillars in the channel, and (B) fabricated PDMS microfluidic chip on glass slide by using O2 plasma.Abbreviation: PDMS, polydimethylsiloxane.
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f2-ijn-10-121: Photographs of (A) photomask for photoresist process for SU-8 molding, inset photo shows the amplified image of pillars in the channel, and (B) fabricated PDMS microfluidic chip on glass slide by using O2 plasma.Abbreviation: PDMS, polydimethylsiloxane.

Mentions: Figure 2A and B shows photographs of the photomask for the photoresist process for SU-8 molding and the fabricated PDMS microfluidic chip on the glass slide by using O2 plasma, respectively. The chip (25 mm ×12 mm) was fabricated using a conventional photo process. The channel width and height were 400 µm and 50 µm, respectively, and the distance between pillars was 25 µm. The elliptical pillar was constructed with a long diameter of 100 µm and a short diameter of 50 µm. The chip had two inlets and two outlets, with two channels. The channels were packed with the beads-QDs-DA. Figure 2A inset photo shows the amplified image of pillars in the channel. The gaps between the pillars were small enough to block the beads but large enough for buffer solution to flow through the channels.


Dopamine-functionalized InP/ZnS quantum dots as fluorescence probes for the detection of adenosine in microfluidic chip.

Ankireddy SR, Kim J - Int J Nanomedicine (2015)

Photographs of (A) photomask for photoresist process for SU-8 molding, inset photo shows the amplified image of pillars in the channel, and (B) fabricated PDMS microfluidic chip on glass slide by using O2 plasma.Abbreviation: PDMS, polydimethylsiloxane.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4554435&req=5

f2-ijn-10-121: Photographs of (A) photomask for photoresist process for SU-8 molding, inset photo shows the amplified image of pillars in the channel, and (B) fabricated PDMS microfluidic chip on glass slide by using O2 plasma.Abbreviation: PDMS, polydimethylsiloxane.
Mentions: Figure 2A and B shows photographs of the photomask for the photoresist process for SU-8 molding and the fabricated PDMS microfluidic chip on the glass slide by using O2 plasma, respectively. The chip (25 mm ×12 mm) was fabricated using a conventional photo process. The channel width and height were 400 µm and 50 µm, respectively, and the distance between pillars was 25 µm. The elliptical pillar was constructed with a long diameter of 100 µm and a short diameter of 50 µm. The chip had two inlets and two outlets, with two channels. The channels were packed with the beads-QDs-DA. Figure 2A inset photo shows the amplified image of pillars in the channel. The gaps between the pillars were small enough to block the beads but large enough for buffer solution to flow through the channels.

Bottom Line: Microbeads are frequently used as solid supports for biomolecules such as proteins and nucleic acids in heterogeneous microfluidic assays.The photoluminescence (PL) intensity of the QDs-DA is quenched by Zn(2+) because of the strong coordination interactions.In the presence of adenosine, Zn(2+) cations preferentially bind to adenosine, and the PL intensity of the QDs-DA is recovered.

View Article: PubMed Central - PubMed

Affiliation: Department of chemical and Biological Engineering, Gachon University, Seongnam, Gyeonggi-Do, South Korea.

ABSTRACT
Microbeads are frequently used as solid supports for biomolecules such as proteins and nucleic acids in heterogeneous microfluidic assays. Chip-based, quantum dot (QD)-bead-biomolecule probes have been used for the detection of various types of DNA. In this study, we developed dopamine (DA)-functionalized InP/ZnS QDs (QDs-DA) as fluorescence probes for the detection of adenosine in microfluidic chips. The photoluminescence (PL) intensity of the QDs-DA is quenched by Zn(2+) because of the strong coordination interactions. In the presence of adenosine, Zn(2+) cations preferentially bind to adenosine, and the PL intensity of the QDs-DA is recovered. A polydimethylsiloxane-based microfluidic chip was fabricated, and adenosine detection was confirmed using QDs-DA probes.

No MeSH data available.


Related in: MedlinePlus