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Selective detection of dopamine in the presence of ascorbic acid via fluorescence quenching of InP/ZnS quantum dots.

Ankireddy SR, Kim J - Int J Nanomedicine (2015)

Bottom Line: The surface of the QDs was modified with l-cysteine by coupling reaction to increase the selectivity of dopamine.The fluorescence of cysteine-capped indium phosphide/zinc sulfide QDs was quenched by dopamine with various concentrations in the presence of ascorbic acid.This method shows good selectivity for dopamine detection, and the detection limit was 5 nM.

View Article: PubMed Central - PubMed

Affiliation: Department of chemical and Biological Engineering, Gachon University, Seongnam, South Korea.

ABSTRACT
Dopamine is a neurotransmitter of the catecholamine family and has many important roles, especially in human brain. Several diseases of the nervous system, such as Parkinson's disease, attention deficit hyperactivity disorder, restless legs syndrome, are believed to be related to deficiency of dopamine. Several studies have been performed to detect dopamine by using electrochemical analysis. In this study, quantum dots (QDs) were used as sensing media for the detection of dopamine. The surface of the QDs was modified with l-cysteine by coupling reaction to increase the selectivity of dopamine. The fluorescence of cysteine-capped indium phosphide/zinc sulfide QDs was quenched by dopamine with various concentrations in the presence of ascorbic acid. This method shows good selectivity for dopamine detection, and the detection limit was 5 nM.

No MeSH data available.


Related in: MedlinePlus

The HRTEM image of the l-Cys-capped InP/ZnS QDs.Note: The inset shows a clear enlarged image of a single InP/ZnS nanocrystal.Abbreviations: HRTEM, high-resolution transmission electron microscopy; l-Cys, l-cysteine; InP/ZnS QDs, indium phosphide/zinc sulfide quantum dots.
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f1-ijn-10-113: The HRTEM image of the l-Cys-capped InP/ZnS QDs.Note: The inset shows a clear enlarged image of a single InP/ZnS nanocrystal.Abbreviations: HRTEM, high-resolution transmission electron microscopy; l-Cys, l-cysteine; InP/ZnS QDs, indium phosphide/zinc sulfide quantum dots.

Mentions: The InP/ZnS QDs were insoluble in the DI water and appeared in the organic phase. After surface modification with l-Cys amino acid, these QDs were readily soluble in DI water and appeared in the water phase. Water-soluble l-Cys-capped InP/ZnS QDs were obtained. High-resolution transmission electron microscopy (HRTEM) was employed to characterize the size and morphology of water-soluble l-Cys-capped InP/ZnS QDs. The QDs were mixed with 1 mL ethanol and sonicated for 10 minutes. After sonication, a few drops of InP/ZnS QDs were placed on to carbon-coated copper grids, allowing the excess solvent to evaporate for HRTEM analysis. Figure 1 shows the HRTEM image of l-Cys-capped InP/ZnS QDs. The figure shows that the QDs are monodispersed with an average diameter of 3–5 nm. The inset shows a clear enlarged image of the single InP/ZnS nanocrystals.


Selective detection of dopamine in the presence of ascorbic acid via fluorescence quenching of InP/ZnS quantum dots.

Ankireddy SR, Kim J - Int J Nanomedicine (2015)

The HRTEM image of the l-Cys-capped InP/ZnS QDs.Note: The inset shows a clear enlarged image of a single InP/ZnS nanocrystal.Abbreviations: HRTEM, high-resolution transmission electron microscopy; l-Cys, l-cysteine; InP/ZnS QDs, indium phosphide/zinc sulfide quantum dots.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4554434&req=5

f1-ijn-10-113: The HRTEM image of the l-Cys-capped InP/ZnS QDs.Note: The inset shows a clear enlarged image of a single InP/ZnS nanocrystal.Abbreviations: HRTEM, high-resolution transmission electron microscopy; l-Cys, l-cysteine; InP/ZnS QDs, indium phosphide/zinc sulfide quantum dots.
Mentions: The InP/ZnS QDs were insoluble in the DI water and appeared in the organic phase. After surface modification with l-Cys amino acid, these QDs were readily soluble in DI water and appeared in the water phase. Water-soluble l-Cys-capped InP/ZnS QDs were obtained. High-resolution transmission electron microscopy (HRTEM) was employed to characterize the size and morphology of water-soluble l-Cys-capped InP/ZnS QDs. The QDs were mixed with 1 mL ethanol and sonicated for 10 minutes. After sonication, a few drops of InP/ZnS QDs were placed on to carbon-coated copper grids, allowing the excess solvent to evaporate for HRTEM analysis. Figure 1 shows the HRTEM image of l-Cys-capped InP/ZnS QDs. The figure shows that the QDs are monodispersed with an average diameter of 3–5 nm. The inset shows a clear enlarged image of the single InP/ZnS nanocrystals.

Bottom Line: The surface of the QDs was modified with l-cysteine by coupling reaction to increase the selectivity of dopamine.The fluorescence of cysteine-capped indium phosphide/zinc sulfide QDs was quenched by dopamine with various concentrations in the presence of ascorbic acid.This method shows good selectivity for dopamine detection, and the detection limit was 5 nM.

View Article: PubMed Central - PubMed

Affiliation: Department of chemical and Biological Engineering, Gachon University, Seongnam, South Korea.

ABSTRACT
Dopamine is a neurotransmitter of the catecholamine family and has many important roles, especially in human brain. Several diseases of the nervous system, such as Parkinson's disease, attention deficit hyperactivity disorder, restless legs syndrome, are believed to be related to deficiency of dopamine. Several studies have been performed to detect dopamine by using electrochemical analysis. In this study, quantum dots (QDs) were used as sensing media for the detection of dopamine. The surface of the QDs was modified with l-cysteine by coupling reaction to increase the selectivity of dopamine. The fluorescence of cysteine-capped indium phosphide/zinc sulfide QDs was quenched by dopamine with various concentrations in the presence of ascorbic acid. This method shows good selectivity for dopamine detection, and the detection limit was 5 nM.

No MeSH data available.


Related in: MedlinePlus