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Graphene oxide-modified ZnO particles: synthesis, characterization, and antibacterial properties.

Zhong L, Yun K - Int J Nanomedicine (2015)

Bottom Line: The morphology of the graphene oxide sheets and ZnO particles was confirmed with field emission scanning electron microscopy and biological atomic force microscopy.Enhanced electrochemical properties were detected with cyclic voltammetry, with a redox peak of the composites at 0.025 mV.After further study of the antibacterial mechanism, we concluded that a vast number of reactive oxygen species formed on the surface of composites, improving antibacterial properties.

View Article: PubMed Central - PubMed

Affiliation: Department of Bionanotechnology, Gachon University, Gyeonggi-do, Republic of Korea.

ABSTRACT
Nanosized ZnO particles with diameters of 15 nm were prepared with a solution precipitation method at low cost and high yield. The synthesis of the particles was functionalized by the organic solvent dimethylformamide, and the particles were covalently bonded to the surface of graphene oxide. The morphology of the graphene oxide sheets and ZnO particles was confirmed with field emission scanning electron microscopy and biological atomic force microscopy. Fourier transform infrared spectroscopy and X-ray diffraction were used to analyze the physical and chemical properties of the ZnO/graphene oxide composites that differed from those of the individual components. Enhanced electrochemical properties were detected with cyclic voltammetry, with a redox peak of the composites at 0.025 mV. Excellent antibacterial activity of ZnO/graphene oxide composites was observed with a microdilution method in which minimum inhibitory concentrations of 6.25 µg/mL for Escherichia coli and Salmonella typhimurium, 12.5 µg/mL for Bacillus subtilis, and 25 µg/mL for Enterococcus faecalis. After further study of the antibacterial mechanism, we concluded that a vast number of reactive oxygen species formed on the surface of composites, improving antibacterial properties.

No MeSH data available.


Related in: MedlinePlus

SEM images of (A) normal Escherichia coli cells with integrated membrane; (B)–(D) ZnO particles, graphene oxide, and ZnO/graphene oxide composites treated E. coli, respectively.Note: After 2 hours, by comparison, ZnO/graphene oxide composites cause more damage than ZnO particles and graphene oxide sheets.Abbreviations: SEM, scanning electron microscopy; ZnO, zinc oxide.
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f11-ijn-10-079: SEM images of (A) normal Escherichia coli cells with integrated membrane; (B)–(D) ZnO particles, graphene oxide, and ZnO/graphene oxide composites treated E. coli, respectively.Note: After 2 hours, by comparison, ZnO/graphene oxide composites cause more damage than ZnO particles and graphene oxide sheets.Abbreviations: SEM, scanning electron microscopy; ZnO, zinc oxide.

Mentions: SEM images show bacterial morphology under different conditions. Figure 11A demonstrates bacterial growth and membrane integrity without any agents. Figure 11B and C shows the destruction of the bacterial surface after 2 hours that is attributed to the antibacterial effects of ZnO particles or graphene oxide sheets because the sides of the bacterial membranes with which they contact are wrinkled. We also observed cytoplasm from destroyed bacteria. Serious damage occurred to bacteria attached to ZnO/graphene oxide composites as shown in Figure 11D, and this result shows that compared with their individual components, ZnO/graphene oxide composites have superior antibacterial activity against E. coli.


Graphene oxide-modified ZnO particles: synthesis, characterization, and antibacterial properties.

Zhong L, Yun K - Int J Nanomedicine (2015)

SEM images of (A) normal Escherichia coli cells with integrated membrane; (B)–(D) ZnO particles, graphene oxide, and ZnO/graphene oxide composites treated E. coli, respectively.Note: After 2 hours, by comparison, ZnO/graphene oxide composites cause more damage than ZnO particles and graphene oxide sheets.Abbreviations: SEM, scanning electron microscopy; ZnO, zinc oxide.
© Copyright Policy
Related In: Results  -  Collection

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Show All Figures
getmorefigures.php?uid=PMC4554433&req=5

f11-ijn-10-079: SEM images of (A) normal Escherichia coli cells with integrated membrane; (B)–(D) ZnO particles, graphene oxide, and ZnO/graphene oxide composites treated E. coli, respectively.Note: After 2 hours, by comparison, ZnO/graphene oxide composites cause more damage than ZnO particles and graphene oxide sheets.Abbreviations: SEM, scanning electron microscopy; ZnO, zinc oxide.
Mentions: SEM images show bacterial morphology under different conditions. Figure 11A demonstrates bacterial growth and membrane integrity without any agents. Figure 11B and C shows the destruction of the bacterial surface after 2 hours that is attributed to the antibacterial effects of ZnO particles or graphene oxide sheets because the sides of the bacterial membranes with which they contact are wrinkled. We also observed cytoplasm from destroyed bacteria. Serious damage occurred to bacteria attached to ZnO/graphene oxide composites as shown in Figure 11D, and this result shows that compared with their individual components, ZnO/graphene oxide composites have superior antibacterial activity against E. coli.

Bottom Line: The morphology of the graphene oxide sheets and ZnO particles was confirmed with field emission scanning electron microscopy and biological atomic force microscopy.Enhanced electrochemical properties were detected with cyclic voltammetry, with a redox peak of the composites at 0.025 mV.After further study of the antibacterial mechanism, we concluded that a vast number of reactive oxygen species formed on the surface of composites, improving antibacterial properties.

View Article: PubMed Central - PubMed

Affiliation: Department of Bionanotechnology, Gachon University, Gyeonggi-do, Republic of Korea.

ABSTRACT
Nanosized ZnO particles with diameters of 15 nm were prepared with a solution precipitation method at low cost and high yield. The synthesis of the particles was functionalized by the organic solvent dimethylformamide, and the particles were covalently bonded to the surface of graphene oxide. The morphology of the graphene oxide sheets and ZnO particles was confirmed with field emission scanning electron microscopy and biological atomic force microscopy. Fourier transform infrared spectroscopy and X-ray diffraction were used to analyze the physical and chemical properties of the ZnO/graphene oxide composites that differed from those of the individual components. Enhanced electrochemical properties were detected with cyclic voltammetry, with a redox peak of the composites at 0.025 mV. Excellent antibacterial activity of ZnO/graphene oxide composites was observed with a microdilution method in which minimum inhibitory concentrations of 6.25 µg/mL for Escherichia coli and Salmonella typhimurium, 12.5 µg/mL for Bacillus subtilis, and 25 µg/mL for Enterococcus faecalis. After further study of the antibacterial mechanism, we concluded that a vast number of reactive oxygen species formed on the surface of composites, improving antibacterial properties.

No MeSH data available.


Related in: MedlinePlus