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Lipid-coated gold nanocomposites for enhanced cancer therapy.

Kang JH, Ko YT - Int J Nanomedicine (2015)

Bottom Line: Nanoparticles and their composites were characterized using particle-size analysis, zeta potential measurements, transmission electron microscopy, UV-visible spectroscopy, and reverse-phase high-performance liquid chromatography, demonstrating successful loading of DTX into the lipid bilayer on the surface of the gold nanoparticles.Differential flow cytometry analysis confirmed the improved cellular uptake of lipid-coated nanocomposites.Our preliminary results show that DTX-loaded anionic lipid-coated gold nanorod (AL_AuNR_DTX) and cationic lipid-coated gold nanoparticle (CL_AuNP_DTX) possess effective tumor cell-suppression abilities and can therefore be considered promising chemotherapeutic agents.

View Article: PubMed Central - PubMed

Affiliation: College of Pharmacy, Gachon University, Incheon, Republic of Korea.

ABSTRACT
The aim of the work reported here was to develop lipid-coated multifunctional nanocomposites composed of drugs and nanoparticles for use in cancer therapy. We incorporated thermosensitive phospholipids onto the surface of anisotropic gold nanoparticles (AuNPs) to further enhance drug delivery, with possible additional applications for in vivo imaging and photothermal cancer therapy. Lipid-coated nanohybrids loaded with the drug docetaxel (DTX) were prepared by a thin-film formation, hydration, and sonication method. Nanoparticles and their composites were characterized using particle-size analysis, zeta potential measurements, transmission electron microscopy, UV-visible spectroscopy, and reverse-phase high-performance liquid chromatography, demonstrating successful loading of DTX into the lipid bilayer on the surface of the gold nanoparticles. Initial in vitro studies using breast-cancer (MCF-7) and melanoma (B16F10) cell lines demonstrated that the drug-containing nanocomposites at equivalent drug concentrations caused significant cytotoxicity compared to free DTX. Differential flow cytometry analysis confirmed the improved cellular uptake of lipid-coated nanocomposites. Our preliminary results show that DTX-loaded anionic lipid-coated gold nanorod (AL_AuNR_DTX) and cationic lipid-coated gold nanoparticle (CL_AuNP_DTX) possess effective tumor cell-suppression abilities and can therefore be considered promising chemotherapeutic agents. Further evaluation of the therapeutic efficacy of these hybrid nanoparticles combined with external near-infrared photothermal treatment is warranted to assess their synergistic anticancer actions and potential bioimaging applications.

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Quantitative cellular uptake measured by liquid chromatography–tandem mass spectrometry (LC-MS/MS).Abbreviations: AL_AuNR_DTX, docetaxel-loaded anionic lipid-coated gold nanorod; CL_AuNP_DTX, cationic lipid-coated gold nanoparticle.
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f6-ijn-10-033: Quantitative cellular uptake measured by liquid chromatography–tandem mass spectrometry (LC-MS/MS).Abbreviations: AL_AuNR_DTX, docetaxel-loaded anionic lipid-coated gold nanorod; CL_AuNP_DTX, cationic lipid-coated gold nanoparticle.

Mentions: The intracellular uptake of DTX in B16F10 and MCF-7 cell lines was compared quantitatively using LC-MS/MS after exposure to the nanocomposite formulations CL_AuNP_DTX and AL_AuNR_DTX. The cellular uptake of DTX over time from the nanocomposite formulations is presented in Figure 6. The maximum DTX concentrations measured after treatment were ~40 and ~22 ng/mL in B16F10 and MCF-7 cells, respectively. There was significantly higher DTX uptake from CL_AuNP_DTX than AL_AuNR_DTX in both cell lines. Different mechanisms could be responsible for the cellular uptake of nanoparticle-loaded DTX from both formulations. DTX uptake in B16F10 cells in particular was higher than the uptake from the same formulation group in MCF-7 cells, possibly because of high cluster of differentiation (CD) 44 expression.47


Lipid-coated gold nanocomposites for enhanced cancer therapy.

Kang JH, Ko YT - Int J Nanomedicine (2015)

Quantitative cellular uptake measured by liquid chromatography–tandem mass spectrometry (LC-MS/MS).Abbreviations: AL_AuNR_DTX, docetaxel-loaded anionic lipid-coated gold nanorod; CL_AuNP_DTX, cationic lipid-coated gold nanoparticle.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4554419&req=5

f6-ijn-10-033: Quantitative cellular uptake measured by liquid chromatography–tandem mass spectrometry (LC-MS/MS).Abbreviations: AL_AuNR_DTX, docetaxel-loaded anionic lipid-coated gold nanorod; CL_AuNP_DTX, cationic lipid-coated gold nanoparticle.
Mentions: The intracellular uptake of DTX in B16F10 and MCF-7 cell lines was compared quantitatively using LC-MS/MS after exposure to the nanocomposite formulations CL_AuNP_DTX and AL_AuNR_DTX. The cellular uptake of DTX over time from the nanocomposite formulations is presented in Figure 6. The maximum DTX concentrations measured after treatment were ~40 and ~22 ng/mL in B16F10 and MCF-7 cells, respectively. There was significantly higher DTX uptake from CL_AuNP_DTX than AL_AuNR_DTX in both cell lines. Different mechanisms could be responsible for the cellular uptake of nanoparticle-loaded DTX from both formulations. DTX uptake in B16F10 cells in particular was higher than the uptake from the same formulation group in MCF-7 cells, possibly because of high cluster of differentiation (CD) 44 expression.47

Bottom Line: Nanoparticles and their composites were characterized using particle-size analysis, zeta potential measurements, transmission electron microscopy, UV-visible spectroscopy, and reverse-phase high-performance liquid chromatography, demonstrating successful loading of DTX into the lipid bilayer on the surface of the gold nanoparticles.Differential flow cytometry analysis confirmed the improved cellular uptake of lipid-coated nanocomposites.Our preliminary results show that DTX-loaded anionic lipid-coated gold nanorod (AL_AuNR_DTX) and cationic lipid-coated gold nanoparticle (CL_AuNP_DTX) possess effective tumor cell-suppression abilities and can therefore be considered promising chemotherapeutic agents.

View Article: PubMed Central - PubMed

Affiliation: College of Pharmacy, Gachon University, Incheon, Republic of Korea.

ABSTRACT
The aim of the work reported here was to develop lipid-coated multifunctional nanocomposites composed of drugs and nanoparticles for use in cancer therapy. We incorporated thermosensitive phospholipids onto the surface of anisotropic gold nanoparticles (AuNPs) to further enhance drug delivery, with possible additional applications for in vivo imaging and photothermal cancer therapy. Lipid-coated nanohybrids loaded with the drug docetaxel (DTX) were prepared by a thin-film formation, hydration, and sonication method. Nanoparticles and their composites were characterized using particle-size analysis, zeta potential measurements, transmission electron microscopy, UV-visible spectroscopy, and reverse-phase high-performance liquid chromatography, demonstrating successful loading of DTX into the lipid bilayer on the surface of the gold nanoparticles. Initial in vitro studies using breast-cancer (MCF-7) and melanoma (B16F10) cell lines demonstrated that the drug-containing nanocomposites at equivalent drug concentrations caused significant cytotoxicity compared to free DTX. Differential flow cytometry analysis confirmed the improved cellular uptake of lipid-coated nanocomposites. Our preliminary results show that DTX-loaded anionic lipid-coated gold nanorod (AL_AuNR_DTX) and cationic lipid-coated gold nanoparticle (CL_AuNP_DTX) possess effective tumor cell-suppression abilities and can therefore be considered promising chemotherapeutic agents. Further evaluation of the therapeutic efficacy of these hybrid nanoparticles combined with external near-infrared photothermal treatment is warranted to assess their synergistic anticancer actions and potential bioimaging applications.

No MeSH data available.


Related in: MedlinePlus