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Lipid-coated gold nanocomposites for enhanced cancer therapy.

Kang JH, Ko YT - Int J Nanomedicine (2015)

Bottom Line: Nanoparticles and their composites were characterized using particle-size analysis, zeta potential measurements, transmission electron microscopy, UV-visible spectroscopy, and reverse-phase high-performance liquid chromatography, demonstrating successful loading of DTX into the lipid bilayer on the surface of the gold nanoparticles.Differential flow cytometry analysis confirmed the improved cellular uptake of lipid-coated nanocomposites.Our preliminary results show that DTX-loaded anionic lipid-coated gold nanorod (AL_AuNR_DTX) and cationic lipid-coated gold nanoparticle (CL_AuNP_DTX) possess effective tumor cell-suppression abilities and can therefore be considered promising chemotherapeutic agents.

View Article: PubMed Central - PubMed

Affiliation: College of Pharmacy, Gachon University, Incheon, Republic of Korea.

ABSTRACT
The aim of the work reported here was to develop lipid-coated multifunctional nanocomposites composed of drugs and nanoparticles for use in cancer therapy. We incorporated thermosensitive phospholipids onto the surface of anisotropic gold nanoparticles (AuNPs) to further enhance drug delivery, with possible additional applications for in vivo imaging and photothermal cancer therapy. Lipid-coated nanohybrids loaded with the drug docetaxel (DTX) were prepared by a thin-film formation, hydration, and sonication method. Nanoparticles and their composites were characterized using particle-size analysis, zeta potential measurements, transmission electron microscopy, UV-visible spectroscopy, and reverse-phase high-performance liquid chromatography, demonstrating successful loading of DTX into the lipid bilayer on the surface of the gold nanoparticles. Initial in vitro studies using breast-cancer (MCF-7) and melanoma (B16F10) cell lines demonstrated that the drug-containing nanocomposites at equivalent drug concentrations caused significant cytotoxicity compared to free DTX. Differential flow cytometry analysis confirmed the improved cellular uptake of lipid-coated nanocomposites. Our preliminary results show that DTX-loaded anionic lipid-coated gold nanorod (AL_AuNR_DTX) and cationic lipid-coated gold nanoparticle (CL_AuNP_DTX) possess effective tumor cell-suppression abilities and can therefore be considered promising chemotherapeutic agents. Further evaluation of the therapeutic efficacy of these hybrid nanoparticles combined with external near-infrared photothermal treatment is warranted to assess their synergistic anticancer actions and potential bioimaging applications.

No MeSH data available.


Related in: MedlinePlus

In vitro release profiles of DTX from AL_AuNR_DTX, CL_AuNP_DTX, and free DTX in phosphate-buffered saline (pH 7.4, 0.14 M NaCl) at 37°C.Abbreviations: AL_AuNR_DTX, docetaxel-loaded anionic lipid-coated gold nanorod; CL_AuNP_DTX, cationic lipid-coated gold nanoparticle; DTX, docetaxel.
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f3-ijn-10-033: In vitro release profiles of DTX from AL_AuNR_DTX, CL_AuNP_DTX, and free DTX in phosphate-buffered saline (pH 7.4, 0.14 M NaCl) at 37°C.Abbreviations: AL_AuNR_DTX, docetaxel-loaded anionic lipid-coated gold nanorod; CL_AuNP_DTX, cationic lipid-coated gold nanoparticle; DTX, docetaxel.

Mentions: Figure 3 shows the accumulated percentage release of DTX from the nanocomposite formulations in the PBS medium containing 0.1% w/v Tween 80. Both formulations showed a controlled release of DTX for longer than 24 hours, compared to the burst release observed with free DTX. After 1 day, ~70% and ~41% of the loaded DTX was released from the CL_AuNP_DTX and AL_AuNR_DTX formulations, respectively. Finally, a total of approximately 75% and 45% of DTX was released from CL_AuNP_DTX and AL_AuNR_DTX, respectively, at the end of the 48-hour study period, which is comparable to various other formulations for DTX delivery, as approximately 70% of DTX is released from these within 48 hours.41,42 The CL_AuNP_DTX system showed significantly higher in vitro release than the AL_AuNR_DTX system (P<0.05). The higher percentage of drug release obtained from the CL_AuNP_DTX system may be explained by the influence of the surface area of the AuNPs used. The monodispersed AuNP formulation (CL_AuNP_DTX) could interact with the release media because of its large surface area, which could enhance the release of DTX, compared to the AuNR formulation (AL_AuNR_DTX).


Lipid-coated gold nanocomposites for enhanced cancer therapy.

Kang JH, Ko YT - Int J Nanomedicine (2015)

In vitro release profiles of DTX from AL_AuNR_DTX, CL_AuNP_DTX, and free DTX in phosphate-buffered saline (pH 7.4, 0.14 M NaCl) at 37°C.Abbreviations: AL_AuNR_DTX, docetaxel-loaded anionic lipid-coated gold nanorod; CL_AuNP_DTX, cationic lipid-coated gold nanoparticle; DTX, docetaxel.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4554419&req=5

f3-ijn-10-033: In vitro release profiles of DTX from AL_AuNR_DTX, CL_AuNP_DTX, and free DTX in phosphate-buffered saline (pH 7.4, 0.14 M NaCl) at 37°C.Abbreviations: AL_AuNR_DTX, docetaxel-loaded anionic lipid-coated gold nanorod; CL_AuNP_DTX, cationic lipid-coated gold nanoparticle; DTX, docetaxel.
Mentions: Figure 3 shows the accumulated percentage release of DTX from the nanocomposite formulations in the PBS medium containing 0.1% w/v Tween 80. Both formulations showed a controlled release of DTX for longer than 24 hours, compared to the burst release observed with free DTX. After 1 day, ~70% and ~41% of the loaded DTX was released from the CL_AuNP_DTX and AL_AuNR_DTX formulations, respectively. Finally, a total of approximately 75% and 45% of DTX was released from CL_AuNP_DTX and AL_AuNR_DTX, respectively, at the end of the 48-hour study period, which is comparable to various other formulations for DTX delivery, as approximately 70% of DTX is released from these within 48 hours.41,42 The CL_AuNP_DTX system showed significantly higher in vitro release than the AL_AuNR_DTX system (P<0.05). The higher percentage of drug release obtained from the CL_AuNP_DTX system may be explained by the influence of the surface area of the AuNPs used. The monodispersed AuNP formulation (CL_AuNP_DTX) could interact with the release media because of its large surface area, which could enhance the release of DTX, compared to the AuNR formulation (AL_AuNR_DTX).

Bottom Line: Nanoparticles and their composites were characterized using particle-size analysis, zeta potential measurements, transmission electron microscopy, UV-visible spectroscopy, and reverse-phase high-performance liquid chromatography, demonstrating successful loading of DTX into the lipid bilayer on the surface of the gold nanoparticles.Differential flow cytometry analysis confirmed the improved cellular uptake of lipid-coated nanocomposites.Our preliminary results show that DTX-loaded anionic lipid-coated gold nanorod (AL_AuNR_DTX) and cationic lipid-coated gold nanoparticle (CL_AuNP_DTX) possess effective tumor cell-suppression abilities and can therefore be considered promising chemotherapeutic agents.

View Article: PubMed Central - PubMed

Affiliation: College of Pharmacy, Gachon University, Incheon, Republic of Korea.

ABSTRACT
The aim of the work reported here was to develop lipid-coated multifunctional nanocomposites composed of drugs and nanoparticles for use in cancer therapy. We incorporated thermosensitive phospholipids onto the surface of anisotropic gold nanoparticles (AuNPs) to further enhance drug delivery, with possible additional applications for in vivo imaging and photothermal cancer therapy. Lipid-coated nanohybrids loaded with the drug docetaxel (DTX) were prepared by a thin-film formation, hydration, and sonication method. Nanoparticles and their composites were characterized using particle-size analysis, zeta potential measurements, transmission electron microscopy, UV-visible spectroscopy, and reverse-phase high-performance liquid chromatography, demonstrating successful loading of DTX into the lipid bilayer on the surface of the gold nanoparticles. Initial in vitro studies using breast-cancer (MCF-7) and melanoma (B16F10) cell lines demonstrated that the drug-containing nanocomposites at equivalent drug concentrations caused significant cytotoxicity compared to free DTX. Differential flow cytometry analysis confirmed the improved cellular uptake of lipid-coated nanocomposites. Our preliminary results show that DTX-loaded anionic lipid-coated gold nanorod (AL_AuNR_DTX) and cationic lipid-coated gold nanoparticle (CL_AuNP_DTX) possess effective tumor cell-suppression abilities and can therefore be considered promising chemotherapeutic agents. Further evaluation of the therapeutic efficacy of these hybrid nanoparticles combined with external near-infrared photothermal treatment is warranted to assess their synergistic anticancer actions and potential bioimaging applications.

No MeSH data available.


Related in: MedlinePlus