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Increased NIH 3T3 fibroblast functions on cell culture dishes which mimic the nanometer fibers of natural tissues.

Bhardwaj G, Webster TJ - Int J Nanomedicine (2015)

Bottom Line: However, increasingly it has been observed that mimicking natural tissue properties (such as chemistry, three-dimensional structure, mechanical properties, etc) in vitro can lead to a better correlation of in vitro to in vivo cellular functions.The following studies compared traditional NIH 3T3 fibroblasts' functions on XanoMatrix scaffolds to standard tissue culture polystyrene.Results found significantly greater fibroblast adhesion and proliferation on XanoMatrix cell culture dishes which mimic the nanoscale geometry of natural tissue fibers with true, tortuous fiber beds creating a robust, consistent, and versatile growth platform.

View Article: PubMed Central - PubMed

Affiliation: Department of Chemical Engineering, Northeastern University, Boston, MA, USA.

ABSTRACT
Traditional flat tissue cell culture dishes have consisted of polystyrene treated with plasma gases for growing, subculturing, and studying cell behavior in vitro. However, increasingly it has been observed that mimicking natural tissue properties (such as chemistry, three-dimensional structure, mechanical properties, etc) in vitro can lead to a better correlation of in vitro to in vivo cellular functions. The following studies compared traditional NIH 3T3 fibroblasts' functions on XanoMatrix scaffolds to standard tissue culture polystyrene. Results found significantly greater fibroblast adhesion and proliferation on XanoMatrix cell culture dishes which mimic the nanoscale geometry of natural tissue fibers with true, tortuous fiber beds creating a robust, consistent, and versatile growth platform. In this manner, this study supports that cell culture dishes which mimic features of natural tissues should be continually studied for a wide range of applications in which mimicking natural cellular functions are important.

No MeSH data available.


Related in: MedlinePlus

Confocal microscopy images of NIH 3T3 fibroblast adhesion.Notes: Confocal microscopy images of NIH 3T3 fibroblast adhesion on day 7 on (A) XanoMatrix; (B) Falcon; and (C) Corning surfaces. Scale bar =60 microns. Magnification 10×.
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f6-ijn-10-5293: Confocal microscopy images of NIH 3T3 fibroblast adhesion.Notes: Confocal microscopy images of NIH 3T3 fibroblast adhesion on day 7 on (A) XanoMatrix; (B) Falcon; and (C) Corning surfaces. Scale bar =60 microns. Magnification 10×.

Mentions: As expected, here, the XanoMatrix cell culture well plates increased fibroblast adhesion and proliferation as compared to the Corning and Falcon cell culture petri dishes since they offered a more biologically-inspired 3D nanofiber structure or a structure that mimics the extracellular matrix in the body (Figures 5 and 6). The XanoMatrix surface also had increased roughness at the nanoscale and hydrophobicity as compared to the other control surfaces used in this study. The interaction between fibroblasts and nanofibrous polymers plays an important role in biotechnology and biomedical applications.


Increased NIH 3T3 fibroblast functions on cell culture dishes which mimic the nanometer fibers of natural tissues.

Bhardwaj G, Webster TJ - Int J Nanomedicine (2015)

Confocal microscopy images of NIH 3T3 fibroblast adhesion.Notes: Confocal microscopy images of NIH 3T3 fibroblast adhesion on day 7 on (A) XanoMatrix; (B) Falcon; and (C) Corning surfaces. Scale bar =60 microns. Magnification 10×.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4554417&req=5

f6-ijn-10-5293: Confocal microscopy images of NIH 3T3 fibroblast adhesion.Notes: Confocal microscopy images of NIH 3T3 fibroblast adhesion on day 7 on (A) XanoMatrix; (B) Falcon; and (C) Corning surfaces. Scale bar =60 microns. Magnification 10×.
Mentions: As expected, here, the XanoMatrix cell culture well plates increased fibroblast adhesion and proliferation as compared to the Corning and Falcon cell culture petri dishes since they offered a more biologically-inspired 3D nanofiber structure or a structure that mimics the extracellular matrix in the body (Figures 5 and 6). The XanoMatrix surface also had increased roughness at the nanoscale and hydrophobicity as compared to the other control surfaces used in this study. The interaction between fibroblasts and nanofibrous polymers plays an important role in biotechnology and biomedical applications.

Bottom Line: However, increasingly it has been observed that mimicking natural tissue properties (such as chemistry, three-dimensional structure, mechanical properties, etc) in vitro can lead to a better correlation of in vitro to in vivo cellular functions.The following studies compared traditional NIH 3T3 fibroblasts' functions on XanoMatrix scaffolds to standard tissue culture polystyrene.Results found significantly greater fibroblast adhesion and proliferation on XanoMatrix cell culture dishes which mimic the nanoscale geometry of natural tissue fibers with true, tortuous fiber beds creating a robust, consistent, and versatile growth platform.

View Article: PubMed Central - PubMed

Affiliation: Department of Chemical Engineering, Northeastern University, Boston, MA, USA.

ABSTRACT
Traditional flat tissue cell culture dishes have consisted of polystyrene treated with plasma gases for growing, subculturing, and studying cell behavior in vitro. However, increasingly it has been observed that mimicking natural tissue properties (such as chemistry, three-dimensional structure, mechanical properties, etc) in vitro can lead to a better correlation of in vitro to in vivo cellular functions. The following studies compared traditional NIH 3T3 fibroblasts' functions on XanoMatrix scaffolds to standard tissue culture polystyrene. Results found significantly greater fibroblast adhesion and proliferation on XanoMatrix cell culture dishes which mimic the nanoscale geometry of natural tissue fibers with true, tortuous fiber beds creating a robust, consistent, and versatile growth platform. In this manner, this study supports that cell culture dishes which mimic features of natural tissues should be continually studied for a wide range of applications in which mimicking natural cellular functions are important.

No MeSH data available.


Related in: MedlinePlus