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Increased NIH 3T3 fibroblast functions on cell culture dishes which mimic the nanometer fibers of natural tissues.

Bhardwaj G, Webster TJ - Int J Nanomedicine (2015)

Bottom Line: However, increasingly it has been observed that mimicking natural tissue properties (such as chemistry, three-dimensional structure, mechanical properties, etc) in vitro can lead to a better correlation of in vitro to in vivo cellular functions.The following studies compared traditional NIH 3T3 fibroblasts' functions on XanoMatrix scaffolds to standard tissue culture polystyrene.Results found significantly greater fibroblast adhesion and proliferation on XanoMatrix cell culture dishes which mimic the nanoscale geometry of natural tissue fibers with true, tortuous fiber beds creating a robust, consistent, and versatile growth platform.

View Article: PubMed Central - PubMed

Affiliation: Department of Chemical Engineering, Northeastern University, Boston, MA, USA.

ABSTRACT
Traditional flat tissue cell culture dishes have consisted of polystyrene treated with plasma gases for growing, subculturing, and studying cell behavior in vitro. However, increasingly it has been observed that mimicking natural tissue properties (such as chemistry, three-dimensional structure, mechanical properties, etc) in vitro can lead to a better correlation of in vitro to in vivo cellular functions. The following studies compared traditional NIH 3T3 fibroblasts' functions on XanoMatrix scaffolds to standard tissue culture polystyrene. Results found significantly greater fibroblast adhesion and proliferation on XanoMatrix cell culture dishes which mimic the nanoscale geometry of natural tissue fibers with true, tortuous fiber beds creating a robust, consistent, and versatile growth platform. In this manner, this study supports that cell culture dishes which mimic features of natural tissues should be continually studied for a wide range of applications in which mimicking natural cellular functions are important.

No MeSH data available.


Related in: MedlinePlus

Scanning electron microscopy images.Notes: Scanning electron microscopy images of the surfaces of (A) Corning; (B) Falcon; and (C) XanoMatrix surfaces of the cell culture petri dishes. Scale bar =500 microns.
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f2-ijn-10-5293: Scanning electron microscopy images.Notes: Scanning electron microscopy images of the surfaces of (A) Corning; (B) Falcon; and (C) XanoMatrix surfaces of the cell culture petri dishes. Scale bar =500 microns.

Mentions: The surface of the XanoMatrix was found to be more rough than the Corning and Falcon petri dishes (Figure 2). The XanoMatrix surface was more hydrophobic in nature as compared to the Corning and Falcon petri dishes (Figure 3). The surface of the XanoMatrix showed the presence of nano-fibers and was 3D in nature as compared to the Corning and Falcon petri dishes (Figure 4). Thus, it was more replicative of an in vivo environment for cell growth and proliferation.


Increased NIH 3T3 fibroblast functions on cell culture dishes which mimic the nanometer fibers of natural tissues.

Bhardwaj G, Webster TJ - Int J Nanomedicine (2015)

Scanning electron microscopy images.Notes: Scanning electron microscopy images of the surfaces of (A) Corning; (B) Falcon; and (C) XanoMatrix surfaces of the cell culture petri dishes. Scale bar =500 microns.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4554417&req=5

f2-ijn-10-5293: Scanning electron microscopy images.Notes: Scanning electron microscopy images of the surfaces of (A) Corning; (B) Falcon; and (C) XanoMatrix surfaces of the cell culture petri dishes. Scale bar =500 microns.
Mentions: The surface of the XanoMatrix was found to be more rough than the Corning and Falcon petri dishes (Figure 2). The XanoMatrix surface was more hydrophobic in nature as compared to the Corning and Falcon petri dishes (Figure 3). The surface of the XanoMatrix showed the presence of nano-fibers and was 3D in nature as compared to the Corning and Falcon petri dishes (Figure 4). Thus, it was more replicative of an in vivo environment for cell growth and proliferation.

Bottom Line: However, increasingly it has been observed that mimicking natural tissue properties (such as chemistry, three-dimensional structure, mechanical properties, etc) in vitro can lead to a better correlation of in vitro to in vivo cellular functions.The following studies compared traditional NIH 3T3 fibroblasts' functions on XanoMatrix scaffolds to standard tissue culture polystyrene.Results found significantly greater fibroblast adhesion and proliferation on XanoMatrix cell culture dishes which mimic the nanoscale geometry of natural tissue fibers with true, tortuous fiber beds creating a robust, consistent, and versatile growth platform.

View Article: PubMed Central - PubMed

Affiliation: Department of Chemical Engineering, Northeastern University, Boston, MA, USA.

ABSTRACT
Traditional flat tissue cell culture dishes have consisted of polystyrene treated with plasma gases for growing, subculturing, and studying cell behavior in vitro. However, increasingly it has been observed that mimicking natural tissue properties (such as chemistry, three-dimensional structure, mechanical properties, etc) in vitro can lead to a better correlation of in vitro to in vivo cellular functions. The following studies compared traditional NIH 3T3 fibroblasts' functions on XanoMatrix scaffolds to standard tissue culture polystyrene. Results found significantly greater fibroblast adhesion and proliferation on XanoMatrix cell culture dishes which mimic the nanoscale geometry of natural tissue fibers with true, tortuous fiber beds creating a robust, consistent, and versatile growth platform. In this manner, this study supports that cell culture dishes which mimic features of natural tissues should be continually studied for a wide range of applications in which mimicking natural cellular functions are important.

No MeSH data available.


Related in: MedlinePlus