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Measurement of creatinine in human plasma using a functional porous polymer structure sensing motif.

Nanda SS, An SS, Yi DK - Int J Nanomedicine (2015)

Bottom Line: PLGA and BMIM chloride formed a functional porous polymer structure (FPPS)-like structure.Creatinine within the FPPS rapidly hydrolyzed and released OH(-), which in turn converted DCFH-DA to DCFH, developing an intense green color or green fluorescence.This DCF(+)-based sensor could detect creatinine levels with detection limit of 5 µM and also measure the creatinine in blood.

View Article: PubMed Central - PubMed

Affiliation: Department of Bionanotechnology, Gachon Medical Research Institute, Gachon University, Seongnam, South Korea.

ABSTRACT
In this study, a new method for detecting creatinine was developed. This novel sensor comprised of two ionic liquids, poly-lactic-co-glycolic acid (PLGA) and 1-butyl-3-methylimidazolium (BMIM) chloride, in the presence of 2',7'-dichlorofluorescein diacetate (DCFH-DA). PLGA and BMIM chloride formed a functional porous polymer structure (FPPS)-like structure. Creatinine within the FPPS rapidly hydrolyzed and released OH(-), which in turn converted DCFH-DA to DCFH, developing an intense green color or green fluorescence. The conversion of DCFH to DCF(+) resulted in swelling of FPPS and increased solubility. This DCF(+)-based sensor could detect creatinine levels with detection limit of 5 µM and also measure the creatinine in blood. This novel method could be used in diagnostic applications for monitoring individuals with renal dysfunction.

No MeSH data available.


Related in: MedlinePlus

FPPS and DCFH-DA were used to screen different biomolecules such as glucose, lactose, and urea along with creatinine.Note: Only creatinine showed green fluorescence under UV-visible light (365 nm) and green color in normal white light.Abbreviations: DCFH-DA, 2′,7′-dichlorofluorescein diacetate; FPPS, functional porous polymer structure; UV, ultraviolet.
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f4-ijn-10-093: FPPS and DCFH-DA were used to screen different biomolecules such as glucose, lactose, and urea along with creatinine.Note: Only creatinine showed green fluorescence under UV-visible light (365 nm) and green color in normal white light.Abbreviations: DCFH-DA, 2′,7′-dichlorofluorescein diacetate; FPPS, functional porous polymer structure; UV, ultraviolet.

Mentions: Several researchers in the past focused on sensing creatinine along with other biomolecules such as glucose, lactose, and urea. In Figure 4, we screened all these biomolecules using the same experimental procedure described earlier. The green color under ultraviolet (UV) light (365 nm excitation) or in normal daylight was not observed. This result suggested that the current method exclusively detects creatinine in blood samples.


Measurement of creatinine in human plasma using a functional porous polymer structure sensing motif.

Nanda SS, An SS, Yi DK - Int J Nanomedicine (2015)

FPPS and DCFH-DA were used to screen different biomolecules such as glucose, lactose, and urea along with creatinine.Note: Only creatinine showed green fluorescence under UV-visible light (365 nm) and green color in normal white light.Abbreviations: DCFH-DA, 2′,7′-dichlorofluorescein diacetate; FPPS, functional porous polymer structure; UV, ultraviolet.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4554416&req=5

f4-ijn-10-093: FPPS and DCFH-DA were used to screen different biomolecules such as glucose, lactose, and urea along with creatinine.Note: Only creatinine showed green fluorescence under UV-visible light (365 nm) and green color in normal white light.Abbreviations: DCFH-DA, 2′,7′-dichlorofluorescein diacetate; FPPS, functional porous polymer structure; UV, ultraviolet.
Mentions: Several researchers in the past focused on sensing creatinine along with other biomolecules such as glucose, lactose, and urea. In Figure 4, we screened all these biomolecules using the same experimental procedure described earlier. The green color under ultraviolet (UV) light (365 nm excitation) or in normal daylight was not observed. This result suggested that the current method exclusively detects creatinine in blood samples.

Bottom Line: PLGA and BMIM chloride formed a functional porous polymer structure (FPPS)-like structure.Creatinine within the FPPS rapidly hydrolyzed and released OH(-), which in turn converted DCFH-DA to DCFH, developing an intense green color or green fluorescence.This DCF(+)-based sensor could detect creatinine levels with detection limit of 5 µM and also measure the creatinine in blood.

View Article: PubMed Central - PubMed

Affiliation: Department of Bionanotechnology, Gachon Medical Research Institute, Gachon University, Seongnam, South Korea.

ABSTRACT
In this study, a new method for detecting creatinine was developed. This novel sensor comprised of two ionic liquids, poly-lactic-co-glycolic acid (PLGA) and 1-butyl-3-methylimidazolium (BMIM) chloride, in the presence of 2',7'-dichlorofluorescein diacetate (DCFH-DA). PLGA and BMIM chloride formed a functional porous polymer structure (FPPS)-like structure. Creatinine within the FPPS rapidly hydrolyzed and released OH(-), which in turn converted DCFH-DA to DCFH, developing an intense green color or green fluorescence. The conversion of DCFH to DCF(+) resulted in swelling of FPPS and increased solubility. This DCF(+)-based sensor could detect creatinine levels with detection limit of 5 µM and also measure the creatinine in blood. This novel method could be used in diagnostic applications for monitoring individuals with renal dysfunction.

No MeSH data available.


Related in: MedlinePlus