Limits...
Measurement of creatinine in human plasma using a functional porous polymer structure sensing motif.

Nanda SS, An SS, Yi DK - Int J Nanomedicine (2015)

Bottom Line: PLGA and BMIM chloride formed a functional porous polymer structure (FPPS)-like structure.Creatinine within the FPPS rapidly hydrolyzed and released OH(-), which in turn converted DCFH-DA to DCFH, developing an intense green color or green fluorescence.This DCF(+)-based sensor could detect creatinine levels with detection limit of 5 µM and also measure the creatinine in blood.

View Article: PubMed Central - PubMed

Affiliation: Department of Bionanotechnology, Gachon Medical Research Institute, Gachon University, Seongnam, South Korea.

ABSTRACT
In this study, a new method for detecting creatinine was developed. This novel sensor comprised of two ionic liquids, poly-lactic-co-glycolic acid (PLGA) and 1-butyl-3-methylimidazolium (BMIM) chloride, in the presence of 2',7'-dichlorofluorescein diacetate (DCFH-DA). PLGA and BMIM chloride formed a functional porous polymer structure (FPPS)-like structure. Creatinine within the FPPS rapidly hydrolyzed and released OH(-), which in turn converted DCFH-DA to DCFH, developing an intense green color or green fluorescence. The conversion of DCFH to DCF(+) resulted in swelling of FPPS and increased solubility. This DCF(+)-based sensor could detect creatinine levels with detection limit of 5 µM and also measure the creatinine in blood. This novel method could be used in diagnostic applications for monitoring individuals with renal dysfunction.

No MeSH data available.


Related in: MedlinePlus

The average size of the pore.Note: It was 18.214 µm on the X-axis, 17.946 µm on the Y-axis, and the diameter of the pore was 25.570 µm.
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4554416&req=5

f3-ijn-10-093: The average size of the pore.Note: It was 18.214 µm on the X-axis, 17.946 µm on the Y-axis, and the diameter of the pore was 25.570 µm.

Mentions: Scanning electron microscopy images of slurry mixture of DCM and PLGA revealed the absence of pores in the mixture in Figure 1A. On the other hand, Figure 1B showed that addition of BMIM chloride resulted in the cross-linked structure of BMIM with PLGA and the creation of pores, which were mainly due to the formation of ester linkages. The mechanism of pore formation is outlined in Figure 2. First, the ester linkages converted DCFH-DA to DCFH. Simultaneously, as creatinine entered into the pore, it would go through hydrolysis to produce OH−. Next, OH− ion could convert DCFH to DCF+. Finally, as the concentration of OH− increased, the DCF+ concentrations also increased and produces green color and fluorescence at 530 nm. The average size of the pore is shown in Figure 3, with X- and Y-axis of 18.214 and 17.946 µm, respectively. The estimated diameter of the pore was 25.570 µm. The surface chemistry of the pore and catalytic activity of the ionic liquid plays a vital role in the effectiveness of the creatinine sensor.


Measurement of creatinine in human plasma using a functional porous polymer structure sensing motif.

Nanda SS, An SS, Yi DK - Int J Nanomedicine (2015)

The average size of the pore.Note: It was 18.214 µm on the X-axis, 17.946 µm on the Y-axis, and the diameter of the pore was 25.570 µm.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4554416&req=5

f3-ijn-10-093: The average size of the pore.Note: It was 18.214 µm on the X-axis, 17.946 µm on the Y-axis, and the diameter of the pore was 25.570 µm.
Mentions: Scanning electron microscopy images of slurry mixture of DCM and PLGA revealed the absence of pores in the mixture in Figure 1A. On the other hand, Figure 1B showed that addition of BMIM chloride resulted in the cross-linked structure of BMIM with PLGA and the creation of pores, which were mainly due to the formation of ester linkages. The mechanism of pore formation is outlined in Figure 2. First, the ester linkages converted DCFH-DA to DCFH. Simultaneously, as creatinine entered into the pore, it would go through hydrolysis to produce OH−. Next, OH− ion could convert DCFH to DCF+. Finally, as the concentration of OH− increased, the DCF+ concentrations also increased and produces green color and fluorescence at 530 nm. The average size of the pore is shown in Figure 3, with X- and Y-axis of 18.214 and 17.946 µm, respectively. The estimated diameter of the pore was 25.570 µm. The surface chemistry of the pore and catalytic activity of the ionic liquid plays a vital role in the effectiveness of the creatinine sensor.

Bottom Line: PLGA and BMIM chloride formed a functional porous polymer structure (FPPS)-like structure.Creatinine within the FPPS rapidly hydrolyzed and released OH(-), which in turn converted DCFH-DA to DCFH, developing an intense green color or green fluorescence.This DCF(+)-based sensor could detect creatinine levels with detection limit of 5 µM and also measure the creatinine in blood.

View Article: PubMed Central - PubMed

Affiliation: Department of Bionanotechnology, Gachon Medical Research Institute, Gachon University, Seongnam, South Korea.

ABSTRACT
In this study, a new method for detecting creatinine was developed. This novel sensor comprised of two ionic liquids, poly-lactic-co-glycolic acid (PLGA) and 1-butyl-3-methylimidazolium (BMIM) chloride, in the presence of 2',7'-dichlorofluorescein diacetate (DCFH-DA). PLGA and BMIM chloride formed a functional porous polymer structure (FPPS)-like structure. Creatinine within the FPPS rapidly hydrolyzed and released OH(-), which in turn converted DCFH-DA to DCFH, developing an intense green color or green fluorescence. The conversion of DCFH to DCF(+) resulted in swelling of FPPS and increased solubility. This DCF(+)-based sensor could detect creatinine levels with detection limit of 5 µM and also measure the creatinine in blood. This novel method could be used in diagnostic applications for monitoring individuals with renal dysfunction.

No MeSH data available.


Related in: MedlinePlus